Article: Characterization of a novel gyrovirus in human stool and chicken meat

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TitleCharacterization of a novel gyrovirus in human stool and chicken meat
AuthorsChu, DKW1
Poon, LLM1
Chiu, SSS1
Chan, KH1
Ng, EM1
Bauer, I2
Cheung, TK1
Ng, IHY1
Guan, Y1
Wang, D2
Peiris, JSM1
KeywordsVirus DNA
Chicken meat
Diarrhea
Feces analysis
Gyrovirus
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
CitationJournal of Clinical Virology, 2012, v. 55 n. 3, p. 209-213 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2012.07.001
AbstractBACKGROUND: Sequence-independent amplification of clinical specimens can lead to the identification of novel pathogens. OBJECTIVES: To identify novel viruses in human stool specimens from patients with diarrhea and to investigate the ecology and clinical significance of such viruses. STUDY DESIGN: Nucleic acid extracted from stool specimens from patients with diarrhea with no known etiology were subjected to random PCR amplification and Roche/454 pyrosequencing. Novel viruses identified were genetically and epidemiologically characterized. RESULTS: Four gyroviruses, chicken anemia virus (CAV), human gyrovirus (HGV)/avian gyrovirus 2 (AGV2), gyrovirus 3 (GyV3) and a novel gyrovirus (tentatively designated as gyrovirus 4 (GyV4)) were identified in human stool specimens. GyV4, as well as CAV and AGV2/HGV were also detected in chicken skin and meat used for human consumption. CONCLUSIONS: A novel gyrovirus (GyV4) was identified in human stool and in chicken meat sold for human consumption. This virus was phylogenetically distinct from previously reported gyroviruses in chicken and humans (chicken anemia virus, human gyrovirus, avian gyrovirus 2 and recently reported gyrovirus 3). The epidemiology and pathogenesis of this virus in humans and in chicken needs to be further investigated.
ISSN1386-6532
2011 Impact Factor: 3.969
2011 SCImago Journal Rankings: 0.364
DOIhttp://dx.doi.org/10.1016/j.jcv.2012.07.001
PubMed Central IDPMC3449218
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChu, DKW
dc.contributor.authorPoon, LLM
dc.contributor.authorChiu, SSS
dc.contributor.authorChan, KH
dc.contributor.authorNg, EM
dc.contributor.authorBauer, I
dc.contributor.authorCheung, TK
dc.contributor.authorNg, IHY
dc.contributor.authorGuan, Y
dc.contributor.authorWang, D
dc.contributor.authorPeiris, JSM
dc.date.accessioned2012-09-20T08:48:03Z
dc.date.available2012-09-20T08:48:03Z
dc.date.issued2012
dc.description.abstractBACKGROUND: Sequence-independent amplification of clinical specimens can lead to the identification of novel pathogens. OBJECTIVES: To identify novel viruses in human stool specimens from patients with diarrhea and to investigate the ecology and clinical significance of such viruses. STUDY DESIGN: Nucleic acid extracted from stool specimens from patients with diarrhea with no known etiology were subjected to random PCR amplification and Roche/454 pyrosequencing. Novel viruses identified were genetically and epidemiologically characterized. RESULTS: Four gyroviruses, chicken anemia virus (CAV), human gyrovirus (HGV)/avian gyrovirus 2 (AGV2), gyrovirus 3 (GyV3) and a novel gyrovirus (tentatively designated as gyrovirus 4 (GyV4)) were identified in human stool specimens. GyV4, as well as CAV and AGV2/HGV were also detected in chicken skin and meat used for human consumption. CONCLUSIONS: A novel gyrovirus (GyV4) was identified in human stool and in chicken meat sold for human consumption. This virus was phylogenetically distinct from previously reported gyroviruses in chicken and humans (chicken anemia virus, human gyrovirus, avian gyrovirus 2 and recently reported gyrovirus 3). The epidemiology and pathogenesis of this virus in humans and in chicken needs to be further investigated.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal of Clinical Virology, 2012, v. 55 n. 3, p. 209-213 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jcv.2012.07.001
dc.identifier.citeulike11804352
dc.identifier.doihttp://dx.doi.org/10.1016/j.jcv.2012.07.001
dc.identifier.epage213
dc.identifier.hkuros207973
dc.identifier.hkuros209428
dc.identifier.issn1386-6532
2011 Impact Factor: 3.969
2011 SCImago Journal Rankings: 0.364
dc.identifier.issue3
dc.identifier.pmcidPMC3449218
dc.identifier.pmid22824231
dc.identifier.scopuseid_2-s2.0-84866349607
dc.identifier.spage209
dc.identifier.urihttp://hdl.handle.net/10722/166779
dc.identifier.volume55
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
dc.publisher.placeNetherlands
dc.relation.ispartofJournal of Clinical Virology
dc.relation.referencesReferences in Scopus
dc.subjectVirus DNA
dc.subjectChicken meat
dc.subjectDiarrhea
dc.subjectFeces analysis
dc.subjectGyrovirus
dc.titleCharacterization of a novel gyrovirus in human stool and chicken meat
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Washington University in St. Louis School of Medicine