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Article: In vivo Matrigel Plug Angiogenesis Assay
Title | In vivo Matrigel Plug Angiogenesis Assay |
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Authors | |
Issue Date | 2012 |
Publisher | Bio-protocol. The Journal's web site is located at http://www.bio-protocol.org/Default.aspx |
Citation | Bio-protocol, 2012, v. 2 n. 18 How to Cite? |
Abstract | The matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce differentiation of a variety of cell types such as hepatocytes, mammary epithelial cells, and endothelial cells. In our case, tumor cells are mixed with the matrigel gel and are injected into the mice. The later immunohistochemistry (IHC) staining with the endothelial marker indicates the presence of the newly formed capillaries in the sectioned gel plugs. |
Persistent Identifier | http://hdl.handle.net/10722/166769 |
ISSN | 2023 Impact Factor: 1.0 2023 SCImago Journal Rankings: 0.505 |
DC Field | Value | Language |
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dc.contributor.author | Lung, HL | en_US |
dc.contributor.author | Lung, ML | en_US |
dc.date.accessioned | 2012-09-20T08:47:28Z | - |
dc.date.available | 2012-09-20T08:47:28Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | Bio-protocol, 2012, v. 2 n. 18 | - |
dc.identifier.issn | 2331-8325 | - |
dc.identifier.uri | http://hdl.handle.net/10722/166769 | - |
dc.description.abstract | The matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce differentiation of a variety of cell types such as hepatocytes, mammary epithelial cells, and endothelial cells. In our case, tumor cells are mixed with the matrigel gel and are injected into the mice. The later immunohistochemistry (IHC) staining with the endothelial marker indicates the presence of the newly formed capillaries in the sectioned gel plugs. | - |
dc.language | eng | en_US |
dc.publisher | Bio-protocol. The Journal's web site is located at http://www.bio-protocol.org/Default.aspx | - |
dc.relation.ispartof | Bio-protocol | - |
dc.title | In vivo Matrigel Plug Angiogenesis Assay | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lung, HL: hllung2@hku.hk | en_US |
dc.identifier.email | Lung, ML: mlilung@hku.hk | en_US |
dc.identifier.authority | Lung, HL=rp00299 | en_US |
dc.identifier.authority | Lung, ML=rp00300 | en_US |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.hkuros | 206875 | en_US |
dc.identifier.volume | 2 | - |
dc.identifier.issue | 18 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 2331-8325 | - |