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Article: In vivo Matrigel Plug Angiogenesis Assay

TitleIn vivo Matrigel Plug Angiogenesis Assay
Authors
Issue Date2012
PublisherBio-protocol. The Journal's web site is located at http://www.bio-protocol.org/Default.aspx
Citation
Bio-protocol, 2012, v. 2 n. 18 How to Cite?
AbstractThe matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce differentiation of a variety of cell types such as hepatocytes, mammary epithelial cells, and endothelial cells. In our case, tumor cells are mixed with the matrigel gel and are injected into the mice. The later immunohistochemistry (IHC) staining with the endothelial marker indicates the presence of the newly formed capillaries in the sectioned gel plugs.
Persistent Identifierhttp://hdl.handle.net/10722/166769
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLung, HLen_US
dc.contributor.authorLung, MLen_US
dc.date.accessioned2012-09-20T08:47:28Z-
dc.date.available2012-09-20T08:47:28Z-
dc.date.issued2012en_US
dc.identifier.citationBio-protocol, 2012, v. 2 n. 18-
dc.identifier.issn2331-8325-
dc.identifier.urihttp://hdl.handle.net/10722/166769-
dc.description.abstractThe matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce differentiation of a variety of cell types such as hepatocytes, mammary epithelial cells, and endothelial cells. In our case, tumor cells are mixed with the matrigel gel and are injected into the mice. The later immunohistochemistry (IHC) staining with the endothelial marker indicates the presence of the newly formed capillaries in the sectioned gel plugs.-
dc.languageengen_US
dc.publisherBio-protocol. The Journal's web site is located at http://www.bio-protocol.org/Default.aspx-
dc.relation.ispartofBio-protocol-
dc.titleIn vivo Matrigel Plug Angiogenesis Assayen_US
dc.typeArticleen_US
dc.identifier.emailLung, HL: hllung2@hku.hken_US
dc.identifier.emailLung, ML: mlilung@hku.hken_US
dc.identifier.authorityLung, HL=rp00299en_US
dc.identifier.authorityLung, ML=rp00300en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros206875en_US
dc.identifier.volume2-
dc.identifier.issue18-
dc.publisher.placeUnited States-

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