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Article: Genotype analyses using SNP (using MALDI-TOF Mass spectrometry) and STR (microsatellite) markers in the determination of zygosity status of Chinese Twins

TitleGenotype analyses using SNP (using MALDI-TOF Mass spectrometry) and STR (microsatellite) markers in the determination of zygosity status of Chinese Twins
Authors
Issue Date2012
PublisherNova Science Publishers, Inc.. The Journal's web site is located at https://www.novapublishers.com/catalog/product_info.php?products_id=18051&osCsid=
Citation
Journal of Biochemistry: Molecular Biology in the Post Genomic Era, 2012, v. 1 n. 1, article no. 3 How to Cite?
AbstractIt has been argued that single nucleotide polymorphisms (SNPs) is a very useful tool complementary to microsatellite analyses in the near future due to its relative ease of automation and interpretation. However, because allele frequencies can vary greatly among populations, it is important to validate and identify the informative SNPs that are useful for sample identification in biomedical and epidemiological studies. We have genotyped 768 individuals (384 pairs of twins) with a panel of SNPs based mostly on the SNPs chosen by an earlier work conducted by Lee et al., 2005 for Koreans, which we hypothesized to be useful for our Chinese subjects. The MALDI-TOF mass spectrometry based iPLEX Gold assay on the MassARRAY® Platform method using Sequenom was used for determination of the zygosity status of the twins. The ABI microsatellite kit (AmpFISTR identifiler PCR amplifcation kit) for measuring 16 loci was used to confirm the zygosity status. We found that some of the SNPs (2/22) used in Lee’s paper were not suitable for our Hong Kong Chinese samples. The zygosity status as determined by the mass spectrometry method can be validated with microsatellite method using 76 samples. In summary, we have studied a panel of 25 SNPs on their effectiveness in distinguishing identical and non-identical twins (768 individuals) for the Hong Kong Chinese population using the iPLEX method of Sequenom. It is important to optimize SNP panels for genotyping depending on the population and the platform used for the studies.
Persistent Identifierhttp://hdl.handle.net/10722/166067
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLim, CKPen_US
dc.contributor.authorYeung, VSYen_US
dc.contributor.authorYeung, TLen_US
dc.contributor.authorTam, ACYen_US
dc.contributor.authorHo, CSHen_US
dc.contributor.authorWong, SWLen_US
dc.contributor.authorChow, BWYen_US
dc.contributor.authorHo, YMen_US
dc.contributor.authorChou, CHNen_US
dc.contributor.authorFung, CHFen_US
dc.contributor.authorWong, CFen_US
dc.contributor.authorWaye, MMYen_US
dc.date.accessioned2012-09-20T08:27:15Z-
dc.date.available2012-09-20T08:27:15Z-
dc.date.issued2012en_US
dc.identifier.citationJournal of Biochemistry: Molecular Biology in the Post Genomic Era, 2012, v. 1 n. 1, article no. 3en_US
dc.identifier.issn2156-5732-
dc.identifier.urihttp://hdl.handle.net/10722/166067-
dc.description.abstractIt has been argued that single nucleotide polymorphisms (SNPs) is a very useful tool complementary to microsatellite analyses in the near future due to its relative ease of automation and interpretation. However, because allele frequencies can vary greatly among populations, it is important to validate and identify the informative SNPs that are useful for sample identification in biomedical and epidemiological studies. We have genotyped 768 individuals (384 pairs of twins) with a panel of SNPs based mostly on the SNPs chosen by an earlier work conducted by Lee et al., 2005 for Koreans, which we hypothesized to be useful for our Chinese subjects. The MALDI-TOF mass spectrometry based iPLEX Gold assay on the MassARRAY® Platform method using Sequenom was used for determination of the zygosity status of the twins. The ABI microsatellite kit (AmpFISTR identifiler PCR amplifcation kit) for measuring 16 loci was used to confirm the zygosity status. We found that some of the SNPs (2/22) used in Lee’s paper were not suitable for our Hong Kong Chinese samples. The zygosity status as determined by the mass spectrometry method can be validated with microsatellite method using 76 samples. In summary, we have studied a panel of 25 SNPs on their effectiveness in distinguishing identical and non-identical twins (768 individuals) for the Hong Kong Chinese population using the iPLEX method of Sequenom. It is important to optimize SNP panels for genotyping depending on the population and the platform used for the studies.-
dc.languageengen_US
dc.publisherNova Science Publishers, Inc.. The Journal's web site is located at https://www.novapublishers.com/catalog/product_info.php?products_id=18051&osCsid=-
dc.relation.ispartofJournal of Biochemistry: Molecular Biology in the Post Genomic Eraen_US
dc.titleGenotype analyses using SNP (using MALDI-TOF Mass spectrometry) and STR (microsatellite) markers in the determination of zygosity status of Chinese Twinsen_US
dc.typeArticleen_US
dc.identifier.emailHo, CSH: shhoc@hkucc.hku.hken_US
dc.identifier.authorityHo, CSH=rp00631en_US
dc.identifier.hkuros206528en_US
dc.identifier.volume1en_US
dc.identifier.issue1, article no. 3-
dc.publisher.placeUnited States-

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