Article: Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR

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TitlePrevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR
AuthorsYang, N2
Qi, H2
Wong, MML2
Wu, RSS1
Kong, RYC2
Issue Date2012
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
CitationMarine Pollution Bulletin, 2012, v. 64 n. 1, p. 164-168 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.10.037
AbstractMarine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. © 2011 Elsevier Ltd.
ISSN0025-326X
2011 Impact Factor: 2.503
2011 SCImago Journal Rankings: 0.098
DOIhttp://dx.doi.org/10.1016/j.marpolbul.2011.10.037
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorYang, N
dc.contributor.authorQi, H
dc.contributor.authorWong, MML
dc.contributor.authorWu, RSS
dc.contributor.authorKong, RYC
dc.date.accessioned2012-09-20T08:25:57Z
dc.date.available2012-09-20T08:25:57Z
dc.date.issued2012
dc.description.abstractMarine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. © 2011 Elsevier Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMarine Pollution Bulletin, 2012, v. 64 n. 1, p. 164-168 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.10.037
dc.identifier.citeulike10087051
dc.identifier.doihttp://dx.doi.org/10.1016/j.marpolbul.2011.10.037
dc.identifier.epage168
dc.identifier.hkuros209782
dc.identifier.hkuros203337
dc.identifier.issn0025-326X
2011 Impact Factor: 2.503
2011 SCImago Journal Rankings: 0.098
dc.identifier.issue1
dc.identifier.pmid22119412
dc.identifier.scopuseid_2-s2.0-83555179093
dc.identifier.spage164
dc.identifier.urihttp://hdl.handle.net/10722/165978
dc.identifier.volume64
dc.languageeng
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
dc.publisher.placeUnited Kingdom
dc.relation.ispartofMarine Pollution Bulletin
dc.relation.referencesReferences in Scopus
dc.subject.meshBiodiversity
dc.subject.meshEnvironmental Monitoring - instrumentation - methods
dc.subject.meshHong Kong
dc.subject.meshHumans
dc.subject.meshNorovirus - classification - genetics - growth & development - isolation & purification
dc.subject.meshPhylogeny
dc.subject.meshReal-Time Polymerase Chain Reaction
dc.subject.meshSeawater - chemistry - virology
dc.subject.meshSewage - analysis - statistics & numerical data - virology
dc.subject.meshWater Pollution - analysis - statistics & numerical data
dc.titlePrevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong