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Article: Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR

TitlePrevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR
Authors
Issue Date2012
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
Citation
Marine Pollution Bulletin, 2012, v. 64 n. 1, p. 164-168 How to Cite?
AbstractMarine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/165978
ISSN
2015 Impact Factor: 3.099
2015 SCImago Journal Rankings: 1.264
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Nen_HK
dc.contributor.authorQi, Hen_HK
dc.contributor.authorWong, MMLen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorKong, RYCen_HK
dc.date.accessioned2012-09-20T08:25:57Z-
dc.date.available2012-09-20T08:25:57Z-
dc.date.issued2012en_HK
dc.identifier.citationMarine Pollution Bulletin, 2012, v. 64 n. 1, p. 164-168en_HK
dc.identifier.issn0025-326Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/165978-
dc.description.abstractMarine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. © 2011 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbulen_HK
dc.relation.ispartofMarine Pollution Bulletinen_HK
dc.subject.meshBiodiversityen_HK
dc.subject.meshEnvironmental Monitoring - instrumentation - methodsen_HK
dc.subject.meshHong Kongen_HK
dc.subject.meshHumansen_HK
dc.subject.meshNorovirus - classification - genetics - growth & development - isolation & purificationen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshReal-Time Polymerase Chain Reactionen_HK
dc.subject.meshSeawater - chemistry - virologyen_HK
dc.subject.meshSewage - analysis - statistics & numerical data - virologyen_HK
dc.subject.meshWater Pollution - analysis - statistics & numerical dataen_HK
dc.titlePrevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCRen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.marpolbul.2011.10.037en_HK
dc.identifier.pmid22119412-
dc.identifier.scopuseid_2-s2.0-83555179093en_HK
dc.identifier.hkuros209782en_US
dc.identifier.hkuros203337-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-83555179093&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume64en_HK
dc.identifier.issue1en_HK
dc.identifier.spage164en_HK
dc.identifier.epage168en_HK
dc.identifier.isiWOS:000299981100034-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, N=54781922300en_HK
dc.identifier.scopusauthoridQi, H=54781674400en_HK
dc.identifier.scopusauthoridWong, MML=35390115400en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridKong, RYC=7005290687en_HK
dc.identifier.citeulike10087051-

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