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Article: Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR
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TitlePrevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR
 
AuthorsYang, N2
Qi, H2
Wong, MML2
Wu, RSS1
Kong, RYC2
 
Issue Date2012
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
 
CitationMarine Pollution Bulletin, 2012, v. 64 n. 1, p. 164-168 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.10.037
 
AbstractMarine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. © 2011 Elsevier Ltd.
 
ISSN0025-326X
2013 Impact Factor: 2.793
2013 SCImago Journal Rankings: 1.197
 
DOIhttp://dx.doi.org/10.1016/j.marpolbul.2011.10.037
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYang, N
 
dc.contributor.authorQi, H
 
dc.contributor.authorWong, MML
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorKong, RYC
 
dc.date.accessioned2012-09-20T08:25:57Z
 
dc.date.available2012-09-20T08:25:57Z
 
dc.date.issued2012
 
dc.description.abstractMarine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health. © 2011 Elsevier Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationMarine Pollution Bulletin, 2012, v. 64 n. 1, p. 164-168 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.10.037
 
dc.identifier.citeulike10087051
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.marpolbul.2011.10.037
 
dc.identifier.epage168
 
dc.identifier.hkuros209782
 
dc.identifier.hkuros203337
 
dc.identifier.issn0025-326X
2013 Impact Factor: 2.793
2013 SCImago Journal Rankings: 1.197
 
dc.identifier.issue1
 
dc.identifier.pmid22119412
 
dc.identifier.scopuseid_2-s2.0-83555179093
 
dc.identifier.spage164
 
dc.identifier.urihttp://hdl.handle.net/10722/165978
 
dc.identifier.volume64
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofMarine Pollution Bulletin
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBiodiversity
 
dc.subject.meshEnvironmental Monitoring - instrumentation - methods
 
dc.subject.meshHong Kong
 
dc.subject.meshHumans
 
dc.subject.meshNorovirus - classification - genetics - growth & development - isolation & purification
 
dc.subject.meshPhylogeny
 
dc.subject.meshReal-Time Polymerase Chain Reaction
 
dc.subject.meshSeawater - chemistry - virology
 
dc.subject.meshSewage - analysis - statistics & numerical data - virology
 
dc.subject.meshWater Pollution - analysis - statistics & numerical data
 
dc.titlePrevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong