Article: A whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish

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TitleA whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish
AuthorsHan, XB2
Lei, ENY2
Lam, MHW2
Wu, RSS1
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
CitationMarine Pollution Bulletin, 2011, v. 63 n. 5-12, p. 160-165 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.04.001
AbstractPolybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5. ng/L, 1. μg/L and 50. μg/L of DE-71 for the duration of the whole life cycle (120. days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined.In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50. μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated.Serum estradiol (E 2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E 2 as well as the ratio of 11-KT/E 2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. © 2011 Elsevier Ltd.
ISSN0025-326X
2011 Impact Factor: 2.503
2011 SCImago Journal Rankings: 0.098
DOIhttp://dx.doi.org/10.1016/j.marpolbul.2011.04.001
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHan, XB
dc.contributor.authorLei, ENY
dc.contributor.authorLam, MHW
dc.contributor.authorWu, RSS
dc.date.accessioned2012-09-20T08:25:56Z
dc.date.available2012-09-20T08:25:56Z
dc.date.issued2011
dc.description.abstractPolybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5. ng/L, 1. μg/L and 50. μg/L of DE-71 for the duration of the whole life cycle (120. days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined.In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50. μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated.Serum estradiol (E 2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E 2 as well as the ratio of 11-KT/E 2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. © 2011 Elsevier Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationMarine Pollution Bulletin, 2011, v. 63 n. 5-12, p. 160-165 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.04.001
dc.identifier.citeulike9280842
dc.identifier.doihttp://dx.doi.org/10.1016/j.marpolbul.2011.04.001
dc.identifier.epage165
dc.identifier.hkuros209725
dc.identifier.issn0025-326X
2011 Impact Factor: 2.503
2011 SCImago Journal Rankings: 0.098
dc.identifier.issue5-12
dc.identifier.pmid21549400
dc.identifier.scopuseid_2-s2.0-79960374393
dc.identifier.spage160
dc.identifier.urihttp://hdl.handle.net/10722/165974
dc.identifier.volume63
dc.languageeng
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
dc.publisher.placeUnited Kingdom
dc.relation.ispartofMarine Pollution Bulletin
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshAromatase - genetics - metabolism
dc.subject.meshBrain - drug effects - metabolism
dc.subject.meshEndocrine Disruptors - toxicity
dc.subject.meshFemale
dc.subject.meshFollicle Stimulating Hormone, beta Subunit - genetics - metabolism
dc.subject.meshGene Expression - drug effects
dc.subject.meshGonadotropin-Releasing Hormone - genetics - metabolism
dc.subject.meshGonads - drug effects - metabolism
dc.subject.meshHalogenated Diphenyl Ethers - toxicity
dc.subject.meshLiver - drug effects - metabolism
dc.subject.meshLuteinizing Hormone, beta Subunit - genetics - metabolism
dc.subject.meshMale
dc.subject.meshPituitary Gland - drug effects - metabolism
dc.subject.meshReceptors, FSH - genetics - metabolism
dc.subject.meshWater Pollutants, Chemical - toxicity
dc.subject.meshZebrafish
dc.subject.meshZebrafish Proteins - genetics - metabolism
dc.titleA whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong