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Article: A whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish

TitleA whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish
Authors
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
Citation
Marine Pollution Bulletin, 2011, v. 63 n. 5-12, p. 160-165 How to Cite?
Abstract
Polybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5. ng/L, 1. μg/L and 50. μg/L of DE-71 for the duration of the whole life cycle (120. days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined.In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50. μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated.Serum estradiol (E 2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E 2 as well as the ratio of 11-KT/E 2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/165974
ISSN
2013 Impact Factor: 2.793
2013 SCImago Journal Rankings: 1.197
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorHan, XBen_HK
dc.contributor.authorLei, ENYen_HK
dc.contributor.authorLam, MHWen_HK
dc.contributor.authorWu, RSSen_HK
dc.date.accessioned2012-09-20T08:25:56Z-
dc.date.available2012-09-20T08:25:56Z-
dc.date.issued2011en_HK
dc.identifier.citationMarine Pollution Bulletin, 2011, v. 63 n. 5-12, p. 160-165en_HK
dc.identifier.issn0025-326Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/165974-
dc.description.abstractPolybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5. ng/L, 1. μg/L and 50. μg/L of DE-71 for the duration of the whole life cycle (120. days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined.In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50. μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated.Serum estradiol (E 2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E 2 as well as the ratio of 11-KT/E 2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. © 2011 Elsevier Ltd.en_HK
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbulen_HK
dc.relation.ispartofMarine Pollution Bulletinen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAromatase - genetics - metabolismen_HK
dc.subject.meshBrain - drug effects - metabolismen_HK
dc.subject.meshEndocrine Disruptors - toxicityen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFollicle Stimulating Hormone, beta Subunit - genetics - metabolismen_HK
dc.subject.meshGene Expression - drug effectsen_HK
dc.subject.meshGonadotropin-Releasing Hormone - genetics - metabolismen_HK
dc.subject.meshGonads - drug effects - metabolismen_HK
dc.subject.meshHalogenated Diphenyl Ethers - toxicityen_HK
dc.subject.meshLiver - drug effects - metabolismen_HK
dc.subject.meshLuteinizing Hormone, beta Subunit - genetics - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshPituitary Gland - drug effects - metabolismen_HK
dc.subject.meshReceptors, FSH - genetics - metabolismen_HK
dc.subject.meshWater Pollutants, Chemical - toxicityen_HK
dc.subject.meshZebrafishen_HK
dc.subject.meshZebrafish Proteins - genetics - metabolismen_HK
dc.titleA whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafishen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.marpolbul.2011.04.001en_HK
dc.identifier.pmid21549400en_HK
dc.identifier.scopuseid_2-s2.0-79960374393en_HK
dc.identifier.hkuros209725en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79960374393&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume63en_HK
dc.identifier.issue5-12en_HK
dc.identifier.spage160en_HK
dc.identifier.epage165en_HK
dc.identifier.isiWOS:000294092700015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHan, XB=37110924600en_HK
dc.identifier.scopusauthoridLei, ENY=36465365000en_HK
dc.identifier.scopusauthoridLam, MHW=7202630175en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.citeulike9280842-

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