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Article: A whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish
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TitleA whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish
 
AuthorsHan, XB2
Lei, ENY2
Lam, MHW2
Wu, RSS1
 
Issue Date2011
 
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
 
CitationMarine Pollution Bulletin, 2011, v. 63 n. 5-12, p. 160-165 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.04.001
 
AbstractPolybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5. ng/L, 1. μg/L and 50. μg/L of DE-71 for the duration of the whole life cycle (120. days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined.In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50. μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated.Serum estradiol (E 2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E 2 as well as the ratio of 11-KT/E 2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. © 2011 Elsevier Ltd.
 
ISSN0025-326X
2013 Impact Factor: 2.793
2013 SCImago Journal Rankings: 1.197
 
DOIhttp://dx.doi.org/10.1016/j.marpolbul.2011.04.001
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHan, XB
 
dc.contributor.authorLei, ENY
 
dc.contributor.authorLam, MHW
 
dc.contributor.authorWu, RSS
 
dc.date.accessioned2012-09-20T08:25:56Z
 
dc.date.available2012-09-20T08:25:56Z
 
dc.date.issued2011
 
dc.description.abstractPolybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5. ng/L, 1. μg/L and 50. μg/L of DE-71 for the duration of the whole life cycle (120. days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined.In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50. μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated.Serum estradiol (E 2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E 2 as well as the ratio of 11-KT/E 2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. © 2011 Elsevier Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMarine Pollution Bulletin, 2011, v. 63 n. 5-12, p. 160-165 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.marpolbul.2011.04.001
 
dc.identifier.citeulike9280842
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.marpolbul.2011.04.001
 
dc.identifier.epage165
 
dc.identifier.hkuros209725
 
dc.identifier.issn0025-326X
2013 Impact Factor: 2.793
2013 SCImago Journal Rankings: 1.197
 
dc.identifier.issue5-12
 
dc.identifier.pmid21549400
 
dc.identifier.scopuseid_2-s2.0-79960374393
 
dc.identifier.spage160
 
dc.identifier.urihttp://hdl.handle.net/10722/165974
 
dc.identifier.volume63
 
dc.languageeng
 
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofMarine Pollution Bulletin
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAromatase - genetics - metabolism
 
dc.subject.meshBrain - drug effects - metabolism
 
dc.subject.meshEndocrine Disruptors - toxicity
 
dc.subject.meshFemale
 
dc.subject.meshFollicle Stimulating Hormone, beta Subunit - genetics - metabolism
 
dc.subject.meshGene Expression - drug effects
 
dc.subject.meshGonadotropin-Releasing Hormone - genetics - metabolism
 
dc.subject.meshGonads - drug effects - metabolism
 
dc.subject.meshHalogenated Diphenyl Ethers - toxicity
 
dc.subject.meshLiver - drug effects - metabolism
 
dc.subject.meshLuteinizing Hormone, beta Subunit - genetics - metabolism
 
dc.subject.meshMale
 
dc.subject.meshPituitary Gland - drug effects - metabolism
 
dc.subject.meshReceptors, FSH - genetics - metabolism
 
dc.subject.meshWater Pollutants, Chemical - toxicity
 
dc.subject.meshZebrafish
 
dc.subject.meshZebrafish Proteins - genetics - metabolism
 
dc.titleA whole life cycle assessment on effects of waterborne PBDEs on gene expression profile along the brain-pituitary-gonad axis and in the liver of zebrafish
 
dc.typeArticle
 
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<description.abstract>Polybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5. ng/L, 1. &#956;g/L and 50. &#956;g/L of DE-71 for the duration of the whole life cycle (120. days, from eggs to adults). The expression of selected genes along the brain-pituitary-gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined.In male fish, up-regulation of GnRH in brain, FSH&#946; and LH&#946; in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3&#946;-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3&#946;-HSD was found in ovary upon exposure to 50. &#956;g/L of DE-71. GnRH in brain, FSH&#946; and LH&#946; in pituitary were also up-regulated, while ER&#946;, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ER&#945;, AR and VTG in males were all down-regulated, while hepatic ER&#945; and AR in female were up-regulated.Serum estradiol (E 2) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E 2 as well as the ratio of 11-KT/E 2 in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones. &#169; 2011 Elsevier Ltd.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong