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Article: Methanogenic Microbial Community Composition of Oily Sludge and Its Enrichment Amended with Alkanes Incubated for Over 500 Days
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TitleMethanogenic Microbial Community Composition of Oily Sludge and Its Enrichment Amended with Alkanes Incubated for Over 500 Days
 
AuthorsWang, LY2
Li, W2
Mbadinga, SM2
Liu, JF2
Gu, JD1
Mu, BZ2
 
Keywordsmethanogenesis
microbial community
n-alkanes
oily sludge
 
Issue Date2012
 
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01490451.asp
 
CitationGeomicrobiology Journal, 2012, v. 29 n. 8, p. 716-726 [How to Cite?]
DOI: http://dx.doi.org/10.1080/01490451.2011.619634
 
AbstractMethanogenic microbial community is responsive to the availability of hydrocarbons and such information is critical for the assessment of hydrocarbon degradation in remediation and also in biologically enhanced recovery of energy from non-producing oil reserves. In this study, methanogenic enrichment cultures from oily sludge amended with n-alkanes (C 15-C 20) showed a development of active methanogenic alkanes-degrading consortium for over a total of 1000 days of incubation at 37°C. Total genomic DNAs were extracted from three types of samples, the original oily sludge (OS), the sludge after incubation for 500 days under methanogenic condition without any external carbon addition (EC), and the enrichment culture from the EC amended with n-alkanes (ET) incubated for another 500 days. The phylogenetic diversities of microbial communities of the three samples were analyzed by PCR amplification of partial 16S rRNA genes. The catabolic genes encoding benzylsuccinate synthase (bssA) and alkylsuccinate synthase (assA) were also examined by PCR amplification. These results provide important evidence in that microbial populations in an oily sludge shifted from methanogenic aromatic compounds degrading communities to potential methanogenic alkane-degrading communities when the enrichment was supplemented with n-alkanes and incubated under anaerobic conditions. © 2012 Copyright Taylor and Francis Group, LLC.
 
ISSN0149-0451
2013 Impact Factor: 1.804
 
DOIhttp://dx.doi.org/10.1080/01490451.2011.619634
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, LY
 
dc.contributor.authorLi, W
 
dc.contributor.authorMbadinga, SM
 
dc.contributor.authorLiu, JF
 
dc.contributor.authorGu, JD
 
dc.contributor.authorMu, BZ
 
dc.date.accessioned2012-09-20T08:25:54Z
 
dc.date.available2012-09-20T08:25:54Z
 
dc.date.issued2012
 
dc.description.abstractMethanogenic microbial community is responsive to the availability of hydrocarbons and such information is critical for the assessment of hydrocarbon degradation in remediation and also in biologically enhanced recovery of energy from non-producing oil reserves. In this study, methanogenic enrichment cultures from oily sludge amended with n-alkanes (C 15-C 20) showed a development of active methanogenic alkanes-degrading consortium for over a total of 1000 days of incubation at 37°C. Total genomic DNAs were extracted from three types of samples, the original oily sludge (OS), the sludge after incubation for 500 days under methanogenic condition without any external carbon addition (EC), and the enrichment culture from the EC amended with n-alkanes (ET) incubated for another 500 days. The phylogenetic diversities of microbial communities of the three samples were analyzed by PCR amplification of partial 16S rRNA genes. The catabolic genes encoding benzylsuccinate synthase (bssA) and alkylsuccinate synthase (assA) were also examined by PCR amplification. These results provide important evidence in that microbial populations in an oily sludge shifted from methanogenic aromatic compounds degrading communities to potential methanogenic alkane-degrading communities when the enrichment was supplemented with n-alkanes and incubated under anaerobic conditions. © 2012 Copyright Taylor and Francis Group, LLC.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationGeomicrobiology Journal, 2012, v. 29 n. 8, p. 716-726 [How to Cite?]
DOI: http://dx.doi.org/10.1080/01490451.2011.619634
 
dc.identifier.doihttp://dx.doi.org/10.1080/01490451.2011.619634
 
dc.identifier.epage726
 
dc.identifier.hkuros209711
 
dc.identifier.hkuros224956
 
dc.identifier.issn0149-0451
2013 Impact Factor: 1.804
 
dc.identifier.issue8
 
dc.identifier.scopuseid_2-s2.0-84863845364
 
dc.identifier.spage716
 
dc.identifier.urihttp://hdl.handle.net/10722/165969
 
dc.identifier.volume29
 
dc.languageeng
 
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01490451.asp
 
dc.publisher.placeUnited States
 
dc.relation.ispartofGeomicrobiology Journal
 
dc.relation.referencesReferences in Scopus
 
dc.subjectmethanogenesis
 
dc.subjectmicrobial community
 
dc.subjectn-alkanes
 
dc.subjectoily sludge
 
dc.titleMethanogenic Microbial Community Composition of Oily Sludge and Its Enrichment Amended with Alkanes Incubated for Over 500 Days
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. East China University of Science and Technology