Article: Methanogenic Microbial Community Composition of Oily Sludge and Its Enrichment Amended with Alkanes Incubated for Over 500 Days

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TitleMethanogenic Microbial Community Composition of Oily Sludge and Its Enrichment Amended with Alkanes Incubated for Over 500 Days
AuthorsWang, LY2
Li, W2
Mbadinga, SM2
Liu, JF2
Gu, JD1
Mu, BZ2
Keywordsmethanogenesis
microbial community
n-alkanes
oily sludge
Issue Date2012
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01490451.asp
CitationGeomicrobiology Journal, 2012, v. 29 n. 8, p. 716-726 [How to Cite?]
DOI: http://dx.doi.org/10.1080/01490451.2011.619634
AbstractMethanogenic microbial community is responsive to the availability of hydrocarbons and such information is critical for the assessment of hydrocarbon degradation in remediation and also in biologically enhanced recovery of energy from non-producing oil reserves. In this study, methanogenic enrichment cultures from oily sludge amended with n-alkanes (C 15-C 20) showed a development of active methanogenic alkanes-degrading consortium for over a total of 1000 days of incubation at 37°C. Total genomic DNAs were extracted from three types of samples, the original oily sludge (OS), the sludge after incubation for 500 days under methanogenic condition without any external carbon addition (EC), and the enrichment culture from the EC amended with n-alkanes (ET) incubated for another 500 days. The phylogenetic diversities of microbial communities of the three samples were analyzed by PCR amplification of partial 16S rRNA genes. The catabolic genes encoding benzylsuccinate synthase (bssA) and alkylsuccinate synthase (assA) were also examined by PCR amplification. These results provide important evidence in that microbial populations in an oily sludge shifted from methanogenic aromatic compounds degrading communities to potential methanogenic alkane-degrading communities when the enrichment was supplemented with n-alkanes and incubated under anaerobic conditions. © 2012 Copyright Taylor and Francis Group, LLC.
ISSN0149-0451
2011 Impact Factor: 2.017
2011 SCImago Journal Rankings: 0.135
DOIhttp://dx.doi.org/10.1080/01490451.2011.619634
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWang, LY
dc.contributor.authorLi, W
dc.contributor.authorMbadinga, SM
dc.contributor.authorLiu, JF
dc.contributor.authorGu, JD
dc.contributor.authorMu, BZ
dc.date.accessioned2012-09-20T08:25:54Z
dc.date.available2012-09-20T08:25:54Z
dc.date.issued2012
dc.description.abstractMethanogenic microbial community is responsive to the availability of hydrocarbons and such information is critical for the assessment of hydrocarbon degradation in remediation and also in biologically enhanced recovery of energy from non-producing oil reserves. In this study, methanogenic enrichment cultures from oily sludge amended with n-alkanes (C 15-C 20) showed a development of active methanogenic alkanes-degrading consortium for over a total of 1000 days of incubation at 37°C. Total genomic DNAs were extracted from three types of samples, the original oily sludge (OS), the sludge after incubation for 500 days under methanogenic condition without any external carbon addition (EC), and the enrichment culture from the EC amended with n-alkanes (ET) incubated for another 500 days. The phylogenetic diversities of microbial communities of the three samples were analyzed by PCR amplification of partial 16S rRNA genes. The catabolic genes encoding benzylsuccinate synthase (bssA) and alkylsuccinate synthase (assA) were also examined by PCR amplification. These results provide important evidence in that microbial populations in an oily sludge shifted from methanogenic aromatic compounds degrading communities to potential methanogenic alkane-degrading communities when the enrichment was supplemented with n-alkanes and incubated under anaerobic conditions. © 2012 Copyright Taylor and Francis Group, LLC.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationGeomicrobiology Journal, 2012, v. 29 n. 8, p. 716-726 [How to Cite?]
DOI: http://dx.doi.org/10.1080/01490451.2011.619634
dc.identifier.doihttp://dx.doi.org/10.1080/01490451.2011.619634
dc.identifier.epage726
dc.identifier.hkuros209711
dc.identifier.issn0149-0451
2011 Impact Factor: 2.017
2011 SCImago Journal Rankings: 0.135
dc.identifier.issue8
dc.identifier.scopuseid_2-s2.0-84863845364
dc.identifier.spage716
dc.identifier.urihttp://hdl.handle.net/10722/165969
dc.identifier.volume29
dc.languageeng
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01490451.asp
dc.publisher.placeUnited States
dc.relation.ispartofGeomicrobiology Journal
dc.relation.referencesReferences in Scopus
dc.rightsPREPRINT This is a preprint of an article whose final and definitive form has been published in the [JOURNAL TITLE] [year of publication] [copyright Taylor & Francis]; [JOURNAL TITLE] is available online at: http://www.informaworld.com/smpp/ with the open URL of your article POSTPRINT ‘This is an electronic version of an article published in [include the complete citation information for the final version of the article as published in the print edition of the journal]. [JOURNAL TITLE] is available online at: http://www.informaworld.com/smpp/ with the open URL of your article.
dc.subjectmethanogenesis
dc.subjectmicrobial community
dc.subjectn-alkanes
dc.subjectoily sludge
dc.titleMethanogenic Microbial Community Composition of Oily Sludge and Its Enrichment Amended with Alkanes Incubated for Over 500 Days
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. East China University of Science and Technology