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Article: Proteomic analysis of hepatic tissue of ciguatoxin (CTX) contaminated coral reef fish Cephalopholis argus and moray eel Gymnothorax undulatus

TitleProteomic analysis of hepatic tissue of ciguatoxin (CTX) contaminated coral reef fish Cephalopholis argus and moray eel Gymnothorax undulatus
Authors
KeywordsCephalopholis argus
CFP
Ciguatoxin
Gymnothorax undulatus
Proteome
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/hal
Citation
Harmful Algae, 2012, v. 13, p. 65-71 How to Cite?
AbstractCiguatera fish poisoning is a global human food-borne illness caused by the consumption of coral reef fish contaminated with ciguatoxins (CTXs). Some reef fishes, such as groupers and moray eels, are more toxic than others. We hypothesized that fish containing CTXs could produce special proteins or detoxification proteins as a strategy for survival during the accumulation of CTXs. The objective of this study was to characterize the differential proteomes of the toxic and nontoxic hepatic tissue of grouper, Cephalopholis argus and moray eel, Gymnothorax undulatus, which had elevated levels of CTXs. A combination of two dimensional electrophoresis and mass spectrum approaches was employed for preliminary screening of the liver proteome of wild-caught individuals. In C. argus with elevated CTXs, the expression level of cytoskeleton proteins was increased, whereas those of ubiquitin enzymes, ATP related enzymes, and telomerase reverse transcriptase were greatly reduced. In CTX-containing G. undulatus, the proteins involved in Ca 2+ binding, detoxification, antiapoptosis, immune defense, enhanced cell survival and proliferation were elevated. In both toxic fish species, the ATP synthase subunit beta and cytochrome c were down-regulated. However further study is needed to assess their potential roles in the resistance mechanism to contamination by CTXs. In conclusion, the comparative proteomic analysis revealed that CTXs induced influx/efflux of Na + or Ca 2+ changes in fish liver, with a concomitant interference with signal transduction, metabolomics processes, detoxification, antiapoptosis, immune defense, enhanced cell survival and proliferation etc. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/165965
ISSN
2015 Impact Factor: 2.664
2015 SCImago Journal Rankings: 1.644
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJiang, XWen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorCheng, SHen_HK
dc.contributor.authorSchlenk, Den_HK
dc.contributor.authorMitcheson, YSDen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorChan, LLen_HK
dc.date.accessioned2012-09-20T08:25:52Z-
dc.date.available2012-09-20T08:25:52Z-
dc.date.issued2012en_HK
dc.identifier.citationHarmful Algae, 2012, v. 13, p. 65-71en_HK
dc.identifier.issn1568-9883en_HK
dc.identifier.urihttp://hdl.handle.net/10722/165965-
dc.description.abstractCiguatera fish poisoning is a global human food-borne illness caused by the consumption of coral reef fish contaminated with ciguatoxins (CTXs). Some reef fishes, such as groupers and moray eels, are more toxic than others. We hypothesized that fish containing CTXs could produce special proteins or detoxification proteins as a strategy for survival during the accumulation of CTXs. The objective of this study was to characterize the differential proteomes of the toxic and nontoxic hepatic tissue of grouper, Cephalopholis argus and moray eel, Gymnothorax undulatus, which had elevated levels of CTXs. A combination of two dimensional electrophoresis and mass spectrum approaches was employed for preliminary screening of the liver proteome of wild-caught individuals. In C. argus with elevated CTXs, the expression level of cytoskeleton proteins was increased, whereas those of ubiquitin enzymes, ATP related enzymes, and telomerase reverse transcriptase were greatly reduced. In CTX-containing G. undulatus, the proteins involved in Ca 2+ binding, detoxification, antiapoptosis, immune defense, enhanced cell survival and proliferation were elevated. In both toxic fish species, the ATP synthase subunit beta and cytochrome c were down-regulated. However further study is needed to assess their potential roles in the resistance mechanism to contamination by CTXs. In conclusion, the comparative proteomic analysis revealed that CTXs induced influx/efflux of Na + or Ca 2+ changes in fish liver, with a concomitant interference with signal transduction, metabolomics processes, detoxification, antiapoptosis, immune defense, enhanced cell survival and proliferation etc. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/halen_HK
dc.relation.ispartofHarmful Algaeen_HK
dc.subjectCephalopholis argusen_HK
dc.subjectCFPen_HK
dc.subjectCiguatoxinen_HK
dc.subjectGymnothorax undulatusen_HK
dc.subjectProteomeen_HK
dc.titleProteomic analysis of hepatic tissue of ciguatoxin (CTX) contaminated coral reef fish Cephalopholis argus and moray eel Gymnothorax undulatusen_HK
dc.typeArticleen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.hal.2011.10.009en_HK
dc.identifier.scopuseid_2-s2.0-84855213656en_HK
dc.identifier.hkuros209628en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84855213656&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.spage65en_HK
dc.identifier.epage71en_HK
dc.identifier.isiWOS:000300132600009-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridJiang, XW=54083158300en_HK
dc.identifier.scopusauthoridLi, X=52563779300en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridCheng, SH=7404684691en_HK
dc.identifier.scopusauthoridSchlenk, D=7006018368en_HK
dc.identifier.scopusauthoridMitcheson, YSD=26634101900en_HK
dc.identifier.scopusauthoridLi, Y=36072770000en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridChan, LL=35757489200en_HK
dc.identifier.citeulike9973245-

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