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Article: Modulation of steroidogenic gene expression and hormone synthesis in H295R cells exposed to PCP and TCP

TitleModulation of steroidogenic gene expression and hormone synthesis in H295R cells exposed to PCP and TCP
Authors
KeywordsCAMP
Chlorophenol
Endocrine-disruption
Gene expression
H295R
Steroid hormone
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicol
Citation
Toxicology, 2011, v. 282 n. 3, p. 146-153 How to Cite?
AbstractChlorophenols (CPs) have been suspected to disrupt the endocrine system and thus affect human and wildlife reproduction but less is known about the underlying mechanism. In this study, we investigated the effects of pentachlorophenol (PCP) and 2,4,6-trichlorophenol (TCP) on human adrenocortical carcinoma cell line (H295R). The H295R cells were exposed to environmentally relevant concentration (0.0, 0.4, 1.1, 3.4 μM) of PCP and TCP for 48. h, and expression of specific genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3βHSD2, 17βHSD4 and StAR was quantitatively measured using real-time polymerase chain reaction. The selected gene expressions were significantly down-regulated compared with those in the control group. Exposure to PCP and TCP significantly decreased production of both testosterone (T) and 17β-estradiol (E2). Furthermore, a dose-dependent decrease of cellular cAMP was observed in H295R cells exposed to both PCP and TCP. A time-course study revealed that the observed selected steroidogenic gene expressions and protein abundance (StAR) are consistent with reduced cellular cAMP concentrations. The results showed that PCP and TCP may inhibit steroidogenesis by disrupting cAMP signaling. The research indicates that H295R cells can be used as an in vitro model for endocrine disruption assay for chlorophenols and the mechanism involvement of disturbing cAMP signaling. © 2011 Elsevier Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/165950
ISSN
2014 Impact Factor: 3.621
2013 SCImago Journal Rankings: 1.196
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, Yen_HK
dc.contributor.authorLiu, Cen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorGiesy, JPen_HK
dc.contributor.authorHecker, Men_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorZhou, Ben_HK
dc.date.accessioned2012-09-20T08:25:46Z-
dc.date.available2012-09-20T08:25:46Z-
dc.date.issued2011en_HK
dc.identifier.citationToxicology, 2011, v. 282 n. 3, p. 146-153en_HK
dc.identifier.issn0300-483Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/165950-
dc.description.abstractChlorophenols (CPs) have been suspected to disrupt the endocrine system and thus affect human and wildlife reproduction but less is known about the underlying mechanism. In this study, we investigated the effects of pentachlorophenol (PCP) and 2,4,6-trichlorophenol (TCP) on human adrenocortical carcinoma cell line (H295R). The H295R cells were exposed to environmentally relevant concentration (0.0, 0.4, 1.1, 3.4 μM) of PCP and TCP for 48. h, and expression of specific genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3βHSD2, 17βHSD4 and StAR was quantitatively measured using real-time polymerase chain reaction. The selected gene expressions were significantly down-regulated compared with those in the control group. Exposure to PCP and TCP significantly decreased production of both testosterone (T) and 17β-estradiol (E2). Furthermore, a dose-dependent decrease of cellular cAMP was observed in H295R cells exposed to both PCP and TCP. A time-course study revealed that the observed selected steroidogenic gene expressions and protein abundance (StAR) are consistent with reduced cellular cAMP concentrations. The results showed that PCP and TCP may inhibit steroidogenesis by disrupting cAMP signaling. The research indicates that H295R cells can be used as an in vitro model for endocrine disruption assay for chlorophenols and the mechanism involvement of disturbing cAMP signaling. © 2011 Elsevier Ireland Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxicolen_HK
dc.relation.ispartofToxicologyen_HK
dc.subjectCAMPen_HK
dc.subjectChlorophenolen_HK
dc.subjectEndocrine-disruptionen_HK
dc.subjectGene expressionen_HK
dc.subjectH295Ren_HK
dc.subjectSteroid hormoneen_HK
dc.subject.meshChlorophenols - toxicity-
dc.subject.meshEndocrine Disruptors - toxicity-
dc.subject.meshGene Expression Regulation - drug effects-
dc.subject.meshGonadal Steroid Hormones - biosynthesis - genetics-
dc.subject.meshPentachlorophenol - toxicity-
dc.titleModulation of steroidogenic gene expression and hormone synthesis in H295R cells exposed to PCP and TCPen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.tox.2011.01.024en_HK
dc.identifier.pmid21296122-
dc.identifier.scopuseid_2-s2.0-79952310970en_HK
dc.identifier.hkuros208998en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952310970&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume282en_HK
dc.identifier.issue3en_HK
dc.identifier.spage146en_HK
dc.identifier.epage153en_HK
dc.identifier.isiWOS:000289327500010-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridMa, Y=27867767600en_HK
dc.identifier.scopusauthoridLiu, C=35248363100en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridGiesy, JP=35459135300en_HK
dc.identifier.scopusauthoridHecker, M=35247848500en_HK
dc.identifier.scopusauthoridZhang, X=36086912900en_HK
dc.identifier.scopusauthoridZhou, B=7401906781en_HK
dc.identifier.citeulike8795310-

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