File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Development of theca specific antisera for the profiling of cell surface proteins in the marine toxic dinoflagellate genus Alexandrium Halim

TitleDevelopment of theca specific antisera for the profiling of cell surface proteins in the marine toxic dinoflagellate genus Alexandrium Halim
Authors
KeywordsA. tamarense
Alexandrium affine
Cell surface protein (CSP)
Paralytic shellfish toxin (PST)
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/hal
Citation
Harmful Algae, 2012, v. 16, p. 58-62 How to Cite?
AbstractRevealing the profile of dinoflagellate cell surface proteins (CSPs) is a crucial step for developing molecular probes for effective identification, separation and enumeration of toxic and non-toxic dinoflagellates. This study aimed to develop theca-specific antibodies against the dinoflagellates Alexandrium affine (non-toxic) and A. tamarense (toxic) to distinguish the two species, and verify if these antibodies would enable the analysis of multiple CSPs for probing phytoplankton's nutrient physiology, and facilitating rapid detection and enumeration of these harmful algal species. Using 2-DE immunoblots, we evaluated the specificity and effectiveness of the theca-specific polyclonal antisera against two types of antigens generated from fresh or fixed whole cell and insoluble cellular fractions, respectively. Our results showed that, of the four cell surface antigens, paraformaldehyde fixed whole cell antigen derived antiserum specifically recognized weakly bound theca-associated CSPs in toxic Alexandrium strain. Using the optimized theca-specific antisera, about 187 and 110 cell surface associated antigenic spots were identified on the 2-DE immunoblots of A. affine and A. tamarense, respectively. This immunoproteomic approach is proven to be very useful for phytoplankton CSP studies, permitting a more in-depth elucidation of the relationship among nutrient condition, bloom dynamic and toxin production of the harmful algae in the marine environment. © 2012 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/165941
ISSN
2015 Impact Factor: 2.664
2015 SCImago Journal Rankings: 1.644
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, LLen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorLeung, KMYen_HK
dc.date.accessioned2012-09-20T08:25:41Z-
dc.date.available2012-09-20T08:25:41Z-
dc.date.issued2012en_HK
dc.identifier.citationHarmful Algae, 2012, v. 16, p. 58-62en_HK
dc.identifier.issn1568-9883en_HK
dc.identifier.urihttp://hdl.handle.net/10722/165941-
dc.description.abstractRevealing the profile of dinoflagellate cell surface proteins (CSPs) is a crucial step for developing molecular probes for effective identification, separation and enumeration of toxic and non-toxic dinoflagellates. This study aimed to develop theca-specific antibodies against the dinoflagellates Alexandrium affine (non-toxic) and A. tamarense (toxic) to distinguish the two species, and verify if these antibodies would enable the analysis of multiple CSPs for probing phytoplankton's nutrient physiology, and facilitating rapid detection and enumeration of these harmful algal species. Using 2-DE immunoblots, we evaluated the specificity and effectiveness of the theca-specific polyclonal antisera against two types of antigens generated from fresh or fixed whole cell and insoluble cellular fractions, respectively. Our results showed that, of the four cell surface antigens, paraformaldehyde fixed whole cell antigen derived antiserum specifically recognized weakly bound theca-associated CSPs in toxic Alexandrium strain. Using the optimized theca-specific antisera, about 187 and 110 cell surface associated antigenic spots were identified on the 2-DE immunoblots of A. affine and A. tamarense, respectively. This immunoproteomic approach is proven to be very useful for phytoplankton CSP studies, permitting a more in-depth elucidation of the relationship among nutrient condition, bloom dynamic and toxin production of the harmful algae in the marine environment. © 2012 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/halen_HK
dc.relation.ispartofHarmful Algaeen_HK
dc.subjectA. tamarenseen_HK
dc.subjectAlexandrium affineen_HK
dc.subjectCell surface protein (CSP)en_HK
dc.subjectParalytic shellfish toxin (PST)en_HK
dc.titleDevelopment of theca specific antisera for the profiling of cell surface proteins in the marine toxic dinoflagellate genus Alexandrium Halimen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, KMY: kmyleung@hku.hken_HK
dc.identifier.authorityLeung, KMY=rp00733en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.hal.2012.01.004en_HK
dc.identifier.scopuseid_2-s2.0-84862823854en_HK
dc.identifier.hkuros207180en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862823854&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.spage58en_HK
dc.identifier.epage62en_HK
dc.identifier.isiWOS:000303298600007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChan, LL=35757489200en_HK
dc.identifier.scopusauthoridLi, X=52563779300en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridLeung, KMY=7401860738en_HK
dc.identifier.citeulike10326275-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats