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Conference Paper: Role of heme oxygenase-1 in remifentanil cardiac preconditioning in rats

TitleRole of heme oxygenase-1 in remifentanil cardiac preconditioning in rats
Authors
Issue Date2011
PublisherAmerican Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm
Citation
The 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011. In Abstract Archive of the American Society of Anesthesiologists, 2011, abstract no. A1486 How to Cite?
AbstractINTRODUCTION: Remifentanil preconditioning (RPC) can protect the heart from ischemia-reperfusion injury (IRI). Heme oxygenase-1 (HO-1) can also reduce the cardiac infarct size during ischemia reperfusion in vivo. Our aim was to investigate whether HO-1 expression participates in RPC cardio protection. METHODS: IRI was induced in male Sprague-Dawley rats (300g) by occluding the left coronary artery for 30 minutes followed by 2 hours of reperfusion. 6μg/kg/min remifentanil as administered in three infusion cycles of 5-min (RPC group). Hemin, an agonist of HO-1, 30mg/kg/d was given intraperitoneally for 2 days before RPC (RPC+hemin group). Tin Protoporphyrin IX (SnPP), which can inhibit HO-1 expression, was injected 0.7 mg/kg/day subcutaneously for 2 days before RPC (RPC+SnPP group). The non-operation group was marked as sham. In the control group, 0.9% saline replaced remifentanil. After the operation, the heart infarct size was determined by 2, 3, 5-triphenyltetrazolium staining. Expression of HO-1, phosphorylated extracellular single regulated kinase (P-Erk), nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNFα) were also measured. RESULTS: Infarct size was significantly attenuated from 48.47±2.0% (Control, n=6) to 35.09±0.3% (RPC, n=6). The RPC protective effect was enhanced by hemin (6.08±1.4%, n=6) and inhibited by Snpp (39.37±1.1%, n=6). The expression of HO-1 in the RPC group (149.02±34.6) and RPC+hemin group (232.94±133.5) were higher than in the control group (109.15±60.3). The expression of HO-1 in the RPC+Snpp group was 72.52±19.2 vs 109.15±60.3 in the control group. The RPC group (326.98±199.6) and RPC+hemin (261.91±274.8) group had an increase in P-Erk concentration compared with control (202.61±120.6). The effect of RPC was blocked by Snpp (P-Erk 184.11±171.7). In addition, both the expression of iNOS and TNFα were decreased significantly in RPC (iNOS 54.86±9.5; TNFα 88.35±11.5) and RPC+hemin (iNOS 52.59±9.8; TNFα 71.93±23.2) but increased in RPC+SnPP (iNOS 82.00±10.8; TNFα 136.11±81.8) compared with control (iNOS 116.75±59.9; TNFα 170.65±71.4). CONCLUSION: Remifentanil protects the heart from IRI through activation of HO-1.
DescriptionTopic: Experimental Circulation: abstract no. A1486
Persistent Identifierhttp://hdl.handle.net/10722/165920

 

DC FieldValueLanguage
dc.contributor.authorJin, Jen_US
dc.contributor.authorIrwin, MGen_US
dc.contributor.authorWong, Gen_US
dc.contributor.authorXia, Zen_US
dc.date.accessioned2012-09-20T08:25:14Z-
dc.date.available2012-09-20T08:25:14Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011. In Abstract Archive of the American Society of Anesthesiologists, 2011, abstract no. A1486en_US
dc.identifier.urihttp://hdl.handle.net/10722/165920-
dc.descriptionTopic: Experimental Circulation: abstract no. A1486-
dc.description.abstractINTRODUCTION: Remifentanil preconditioning (RPC) can protect the heart from ischemia-reperfusion injury (IRI). Heme oxygenase-1 (HO-1) can also reduce the cardiac infarct size during ischemia reperfusion in vivo. Our aim was to investigate whether HO-1 expression participates in RPC cardio protection. METHODS: IRI was induced in male Sprague-Dawley rats (300g) by occluding the left coronary artery for 30 minutes followed by 2 hours of reperfusion. 6μg/kg/min remifentanil as administered in three infusion cycles of 5-min (RPC group). Hemin, an agonist of HO-1, 30mg/kg/d was given intraperitoneally for 2 days before RPC (RPC+hemin group). Tin Protoporphyrin IX (SnPP), which can inhibit HO-1 expression, was injected 0.7 mg/kg/day subcutaneously for 2 days before RPC (RPC+SnPP group). The non-operation group was marked as sham. In the control group, 0.9% saline replaced remifentanil. After the operation, the heart infarct size was determined by 2, 3, 5-triphenyltetrazolium staining. Expression of HO-1, phosphorylated extracellular single regulated kinase (P-Erk), nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNFα) were also measured. RESULTS: Infarct size was significantly attenuated from 48.47±2.0% (Control, n=6) to 35.09±0.3% (RPC, n=6). The RPC protective effect was enhanced by hemin (6.08±1.4%, n=6) and inhibited by Snpp (39.37±1.1%, n=6). The expression of HO-1 in the RPC group (149.02±34.6) and RPC+hemin group (232.94±133.5) were higher than in the control group (109.15±60.3). The expression of HO-1 in the RPC+Snpp group was 72.52±19.2 vs 109.15±60.3 in the control group. The RPC group (326.98±199.6) and RPC+hemin (261.91±274.8) group had an increase in P-Erk concentration compared with control (202.61±120.6). The effect of RPC was blocked by Snpp (P-Erk 184.11±171.7). In addition, both the expression of iNOS and TNFα were decreased significantly in RPC (iNOS 54.86±9.5; TNFα 88.35±11.5) and RPC+hemin (iNOS 52.59±9.8; TNFα 71.93±23.2) but increased in RPC+SnPP (iNOS 82.00±10.8; TNFα 136.11±81.8) compared with control (iNOS 116.75±59.9; TNFα 170.65±71.4). CONCLUSION: Remifentanil protects the heart from IRI through activation of HO-1.-
dc.languageengen_US
dc.publisherAmerican Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm-
dc.relation.ispartofAbstract Archive of the American Society of Anesthesiologistsen_US
dc.titleRole of heme oxygenase-1 in remifentanil cardiac preconditioning in ratsen_US
dc.typeConference_Paperen_US
dc.identifier.emailJin, J: jinjiqin@hku.hken_US
dc.identifier.emailIrwin, MG: mgirwin@hku.hken_US
dc.identifier.emailWong, G: gordon@hku.hken_US
dc.identifier.emailXia, Z: zyxia@hkucc.hku.hk-
dc.identifier.authorityIrwin, MG=rp00390en_US
dc.identifier.authorityWong, G=rp00523en_US
dc.identifier.authorityXia, Z=rp00532en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros209800en_US
dc.publisher.placeUnited States-
dc.customcontrol.immutablesml 130408-

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