File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: Propofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property
Title | Propofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property |
---|---|
Authors | |
Issue Date | 2011 |
Publisher | American Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm |
Citation | The 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011 How to Cite? |
Abstract | BACKGROUND: Propofol, an intravenous anesthetic that possesses antioxidant and peroxynitrite-scavenging properties, has been shown to confer cardioprotection in both experimental and clinical settings, but the precise mechanism is unclear. Reduction of peroxynitrite production (1) has been shown to play a critical role in hypoxia postconditioning mediated cardioprotection in cultured cardiomyocytes. We, therefore, hypothesize that propofol can confer postconditioning (PPC) cardioprotection via its peroxynitrite scavenging property. METHODS: With ethics approval, isolated and Langendorff-perfused hearts from male Sprague Dawley rats were exposed to 30 min of global ischemia followed by 120 min of reperfusion. During the first 15 min of reperfusion, hearts (n=6-8 per group) were either untreated (control group), or respectively treated with 50 µmol/L of propofol (PPC group), 0.1mmol/ 3-morpholino sydnonimine (SIN-1, a peroxynitrite donor which produces nitric oxide and superoxide anion simultaneously in aqueous solution, SIN group), 20 mg/L of the peroxynitrite decomposer FeTPPs (FeTPPs group), the combinations of propofol with SIN-1 (PPC+SIN) or with FeTPPs (PPC+FeTPPs groups). Myocardial infarct size (IS) was assessed by triphenyltetrazolium staining. Data are expressed as mean ± SD and were analyzed with ANOVA. RESULTS: Postischemic myocardial infarct size (IS) was 36.7±4.7% in the control group. Propofol postconditioning (PPC) significantly reduced IS (28.1±7.0%, P<0.05 vs. control). However, the infarct-sparing effect of PPC was cancelled by co-administration of SIN-1 (IS: 34.5 ±3.9% in PPC+SIN group, P>0.1 vs. control) but facilitated by co-administration of FeTPPs (IS: 15.1±3.8% in PPC+FeTPPs groups, P<0.01 vs. PPC). SIN-1 when given alone during early reperfusion exacerbated postischemic IS (50.9±1.1%, P<0.05 vs. control), while FeTPPs attenuated myocardial IS (21.5 ±8.6%, P<0.01 vs. control). CONCLUSION: It is concluded that propofol can confer postconditioning-like cardioprotection in vitro and that scavenging of peroxynitrite during early reperfusion may represent an important mechanism by which propofol postconditioning confers cardioprotection. |
Description | Topic: Experimental Circulation: abstract no. A537 |
Persistent Identifier | http://hdl.handle.net/10722/165919 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mao, X | en_US |
dc.contributor.author | Wong, G | en_US |
dc.contributor.author | Huang, Z | en_US |
dc.contributor.author | Irwin, MG | en_US |
dc.contributor.author | Xia, Z | en_US |
dc.date.accessioned | 2012-09-20T08:25:13Z | - |
dc.date.available | 2012-09-20T08:25:13Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | The 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/165919 | - |
dc.description | Topic: Experimental Circulation: abstract no. A537 | - |
dc.description.abstract | BACKGROUND: Propofol, an intravenous anesthetic that possesses antioxidant and peroxynitrite-scavenging properties, has been shown to confer cardioprotection in both experimental and clinical settings, but the precise mechanism is unclear. Reduction of peroxynitrite production (1) has been shown to play a critical role in hypoxia postconditioning mediated cardioprotection in cultured cardiomyocytes. We, therefore, hypothesize that propofol can confer postconditioning (PPC) cardioprotection via its peroxynitrite scavenging property. METHODS: With ethics approval, isolated and Langendorff-perfused hearts from male Sprague Dawley rats were exposed to 30 min of global ischemia followed by 120 min of reperfusion. During the first 15 min of reperfusion, hearts (n=6-8 per group) were either untreated (control group), or respectively treated with 50 µmol/L of propofol (PPC group), 0.1mmol/ 3-morpholino sydnonimine (SIN-1, a peroxynitrite donor which produces nitric oxide and superoxide anion simultaneously in aqueous solution, SIN group), 20 mg/L of the peroxynitrite decomposer FeTPPs (FeTPPs group), the combinations of propofol with SIN-1 (PPC+SIN) or with FeTPPs (PPC+FeTPPs groups). Myocardial infarct size (IS) was assessed by triphenyltetrazolium staining. Data are expressed as mean ± SD and were analyzed with ANOVA. RESULTS: Postischemic myocardial infarct size (IS) was 36.7±4.7% in the control group. Propofol postconditioning (PPC) significantly reduced IS (28.1±7.0%, P<0.05 vs. control). However, the infarct-sparing effect of PPC was cancelled by co-administration of SIN-1 (IS: 34.5 ±3.9% in PPC+SIN group, P>0.1 vs. control) but facilitated by co-administration of FeTPPs (IS: 15.1±3.8% in PPC+FeTPPs groups, P<0.01 vs. PPC). SIN-1 when given alone during early reperfusion exacerbated postischemic IS (50.9±1.1%, P<0.05 vs. control), while FeTPPs attenuated myocardial IS (21.5 ±8.6%, P<0.01 vs. control). CONCLUSION: It is concluded that propofol can confer postconditioning-like cardioprotection in vitro and that scavenging of peroxynitrite during early reperfusion may represent an important mechanism by which propofol postconditioning confers cardioprotection. | - |
dc.language | eng | en_US |
dc.publisher | American Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm | - |
dc.relation.ispartof | Annual Meeting of the American Society of Anesthesiologists, ASA 2011 | en_US |
dc.title | Propofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Mao, X: h0994071@hkusuc.hku.hk | en_US |
dc.identifier.email | Wong, G: gordon@hku.hk | en_US |
dc.identifier.email | Huang, Z: zyhuang@hkucc.hku.hk | en_US |
dc.identifier.email | Irwin, MG: mgirwin@hku.hk | en_US |
dc.identifier.email | Xia, Z: zyxia@hkucc.hku.hk | - |
dc.identifier.authority | Wong, G=rp00523 | en_US |
dc.identifier.authority | Irwin, MG=rp00390 | en_US |
dc.identifier.authority | Xia, Z=rp00532 | en_US |
dc.identifier.hkuros | 209799 | en_US |
dc.publisher.place | United States | - |
dc.customcontrol.immutable | sml 130408 | - |