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Conference Paper: Propofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property

TitlePropofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property
Authors
Issue Date2011
PublisherAmerican Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm
Citation
The 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011. In Abstract Archive of the American Society of Anesthesiologists, 2011, abstract no. A537 How to Cite?
AbstractBACKGROUND: Propofol, an intravenous anesthetic that possesses antioxidant and peroxynitrite-scavenging properties, has been shown to confer cardioprotection in both experimental and clinical settings, but the precise mechanism is unclear. Reduction of peroxynitrite production (1) has been shown to play a critical role in hypoxia postconditioning mediated cardioprotection in cultured cardiomyocytes. We, therefore, hypothesize that propofol can confer postconditioning (PPC) cardioprotection via its peroxynitrite scavenging property. METHODS: With ethics approval, isolated and Langendorff-perfused hearts from male Sprague Dawley rats were exposed to 30 min of global ischemia followed by 120 min of reperfusion. During the first 15 min of reperfusion, hearts (n=6-8 per group) were either untreated (control group), or respectively treated with 50 µmol/L of propofol (PPC group), 0.1mmol/ 3-morpholino sydnonimine (SIN-1, a peroxynitrite donor which produces nitric oxide and superoxide anion simultaneously in aqueous solution, SIN group), 20 mg/L of the peroxynitrite decomposer FeTPPs (FeTPPs group), the combinations of propofol with SIN-1 (PPC+SIN) or with FeTPPs (PPC+FeTPPs groups). Myocardial infarct size (IS) was assessed by triphenyltetrazolium staining. Data are expressed as mean ± SD and were analyzed with ANOVA. RESULTS: Postischemic myocardial infarct size (IS) was 36.7±4.7% in the control group. Propofol postconditioning (PPC) significantly reduced IS (28.1±7.0%, P<0.05 vs. control). However, the infarct-sparing effect of PPC was cancelled by co-administration of SIN-1 (IS: 34.5 ±3.9% in PPC+SIN group, P>0.1 vs. control) but facilitated by co-administration of FeTPPs (IS: 15.1±3.8% in PPC+FeTPPs groups, P<0.01 vs. PPC). SIN-1 when given alone during early reperfusion exacerbated postischemic IS (50.9±1.1%, P<0.05 vs. control), while FeTPPs attenuated myocardial IS (21.5 ±8.6%, P<0.01 vs. control). CONCLUSION: It is concluded that propofol can confer postconditioning-like cardioprotection in vitro and that scavenging of peroxynitrite during early reperfusion may represent an important mechanism by which propofol postconditioning confers cardioprotection.
DescriptionTopic: Experimental Circulation: abstract no. A537
Persistent Identifierhttp://hdl.handle.net/10722/165919

 

DC FieldValueLanguage
dc.contributor.authorMao, Xen_US
dc.contributor.authorWong, Gen_US
dc.contributor.authorHuang, Zen_US
dc.contributor.authorIrwin, MGen_US
dc.contributor.authorXia, Zen_US
dc.date.accessioned2012-09-20T08:25:13Z-
dc.date.available2012-09-20T08:25:13Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011. In Abstract Archive of the American Society of Anesthesiologists, 2011, abstract no. A537en_US
dc.identifier.urihttp://hdl.handle.net/10722/165919-
dc.descriptionTopic: Experimental Circulation: abstract no. A537-
dc.description.abstractBACKGROUND: Propofol, an intravenous anesthetic that possesses antioxidant and peroxynitrite-scavenging properties, has been shown to confer cardioprotection in both experimental and clinical settings, but the precise mechanism is unclear. Reduction of peroxynitrite production (1) has been shown to play a critical role in hypoxia postconditioning mediated cardioprotection in cultured cardiomyocytes. We, therefore, hypothesize that propofol can confer postconditioning (PPC) cardioprotection via its peroxynitrite scavenging property. METHODS: With ethics approval, isolated and Langendorff-perfused hearts from male Sprague Dawley rats were exposed to 30 min of global ischemia followed by 120 min of reperfusion. During the first 15 min of reperfusion, hearts (n=6-8 per group) were either untreated (control group), or respectively treated with 50 µmol/L of propofol (PPC group), 0.1mmol/ 3-morpholino sydnonimine (SIN-1, a peroxynitrite donor which produces nitric oxide and superoxide anion simultaneously in aqueous solution, SIN group), 20 mg/L of the peroxynitrite decomposer FeTPPs (FeTPPs group), the combinations of propofol with SIN-1 (PPC+SIN) or with FeTPPs (PPC+FeTPPs groups). Myocardial infarct size (IS) was assessed by triphenyltetrazolium staining. Data are expressed as mean ± SD and were analyzed with ANOVA. RESULTS: Postischemic myocardial infarct size (IS) was 36.7±4.7% in the control group. Propofol postconditioning (PPC) significantly reduced IS (28.1±7.0%, P<0.05 vs. control). However, the infarct-sparing effect of PPC was cancelled by co-administration of SIN-1 (IS: 34.5 ±3.9% in PPC+SIN group, P>0.1 vs. control) but facilitated by co-administration of FeTPPs (IS: 15.1±3.8% in PPC+FeTPPs groups, P<0.01 vs. PPC). SIN-1 when given alone during early reperfusion exacerbated postischemic IS (50.9±1.1%, P<0.05 vs. control), while FeTPPs attenuated myocardial IS (21.5 ±8.6%, P<0.01 vs. control). CONCLUSION: It is concluded that propofol can confer postconditioning-like cardioprotection in vitro and that scavenging of peroxynitrite during early reperfusion may represent an important mechanism by which propofol postconditioning confers cardioprotection.-
dc.languageengen_US
dc.publisherAmerican Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm-
dc.relation.ispartofAbstract Archive of the American Society of Anesthesiologistsen_US
dc.titlePropofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging propertyen_US
dc.typeConference_Paperen_US
dc.identifier.emailMao, X: h0994071@hkusuc.hku.hken_US
dc.identifier.emailWong, G: gordon@hku.hken_US
dc.identifier.emailHuang, Z: zyhuang@hkucc.hku.hken_US
dc.identifier.emailIrwin, MG: mgirwin@hku.hken_US
dc.identifier.emailXia, Z: zyxia@hkucc.hku.hk-
dc.identifier.authorityWong, G=rp00523en_US
dc.identifier.authorityIrwin, MG=rp00390en_US
dc.identifier.authorityXia, Z=rp00532en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros209799en_US
dc.publisher.placeUnited States-
dc.customcontrol.immutablesml 130408-

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