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Conference Paper: Propofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property
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TitlePropofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property
 
AuthorsMao, X
Wong, G
Huang, Z
Irwin, MG
Xia, Z
 
Issue Date2011
 
PublisherAmerican Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm
 
CitationThe 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011. In Abstract Archive of the American Society of Anesthesiologists, 2011, abstract no. A537 [How to Cite?]
 
AbstractBACKGROUND: Propofol, an intravenous anesthetic that possesses antioxidant and peroxynitrite-scavenging properties, has been shown to confer cardioprotection in both experimental and clinical settings, but the precise mechanism is unclear. Reduction of peroxynitrite production (1) has been shown to play a critical role in hypoxia postconditioning mediated cardioprotection in cultured cardiomyocytes. We, therefore, hypothesize that propofol can confer postconditioning (PPC) cardioprotection via its peroxynitrite scavenging property. METHODS: With ethics approval, isolated and Langendorff-perfused hearts from male Sprague Dawley rats were exposed to 30 min of global ischemia followed by 120 min of reperfusion. During the first 15 min of reperfusion, hearts (n=6-8 per group) were either untreated (control group), or respectively treated with 50 µmol/L of propofol (PPC group), 0.1mmol/ 3-morpholino sydnonimine (SIN-1, a peroxynitrite donor which produces nitric oxide and superoxide anion simultaneously in aqueous solution, SIN group), 20 mg/L of the peroxynitrite decomposer FeTPPs (FeTPPs group), the combinations of propofol with SIN-1 (PPC+SIN) or with FeTPPs (PPC+FeTPPs groups). Myocardial infarct size (IS) was assessed by triphenyltetrazolium staining. Data are expressed as mean ± SD and were analyzed with ANOVA. RESULTS: Postischemic myocardial infarct size (IS) was 36.7±4.7% in the control group. Propofol postconditioning (PPC) significantly reduced IS (28.1±7.0%, P<0.05 vs. control). However, the infarct-sparing effect of PPC was cancelled by co-administration of SIN-1 (IS: 34.5 ±3.9% in PPC+SIN group, P>0.1 vs. control) but facilitated by co-administration of FeTPPs (IS: 15.1±3.8% in PPC+FeTPPs groups, P<0.01 vs. PPC). SIN-1 when given alone during early reperfusion exacerbated postischemic IS (50.9±1.1%, P<0.05 vs. control), while FeTPPs attenuated myocardial IS (21.5 ±8.6%, P<0.01 vs. control). CONCLUSION: It is concluded that propofol can confer postconditioning-like cardioprotection in vitro and that scavenging of peroxynitrite during early reperfusion may represent an important mechanism by which propofol postconditioning confers cardioprotection.
 
DescriptionTopic: Experimental Circulation: abstract no. A537
 
DC FieldValue
dc.contributor.authorMao, X
 
dc.contributor.authorWong, G
 
dc.contributor.authorHuang, Z
 
dc.contributor.authorIrwin, MG
 
dc.contributor.authorXia, Z
 
dc.date.accessioned2012-09-20T08:25:13Z
 
dc.date.available2012-09-20T08:25:13Z
 
dc.date.issued2011
 
dc.description.abstractBACKGROUND: Propofol, an intravenous anesthetic that possesses antioxidant and peroxynitrite-scavenging properties, has been shown to confer cardioprotection in both experimental and clinical settings, but the precise mechanism is unclear. Reduction of peroxynitrite production (1) has been shown to play a critical role in hypoxia postconditioning mediated cardioprotection in cultured cardiomyocytes. We, therefore, hypothesize that propofol can confer postconditioning (PPC) cardioprotection via its peroxynitrite scavenging property. METHODS: With ethics approval, isolated and Langendorff-perfused hearts from male Sprague Dawley rats were exposed to 30 min of global ischemia followed by 120 min of reperfusion. During the first 15 min of reperfusion, hearts (n=6-8 per group) were either untreated (control group), or respectively treated with 50 µmol/L of propofol (PPC group), 0.1mmol/ 3-morpholino sydnonimine (SIN-1, a peroxynitrite donor which produces nitric oxide and superoxide anion simultaneously in aqueous solution, SIN group), 20 mg/L of the peroxynitrite decomposer FeTPPs (FeTPPs group), the combinations of propofol with SIN-1 (PPC+SIN) or with FeTPPs (PPC+FeTPPs groups). Myocardial infarct size (IS) was assessed by triphenyltetrazolium staining. Data are expressed as mean ± SD and were analyzed with ANOVA. RESULTS: Postischemic myocardial infarct size (IS) was 36.7±4.7% in the control group. Propofol postconditioning (PPC) significantly reduced IS (28.1±7.0%, P<0.05 vs. control). However, the infarct-sparing effect of PPC was cancelled by co-administration of SIN-1 (IS: 34.5 ±3.9% in PPC+SIN group, P>0.1 vs. control) but facilitated by co-administration of FeTPPs (IS: 15.1±3.8% in PPC+FeTPPs groups, P<0.01 vs. PPC). SIN-1 when given alone during early reperfusion exacerbated postischemic IS (50.9±1.1%, P<0.05 vs. control), while FeTPPs attenuated myocardial IS (21.5 ±8.6%, P<0.01 vs. control). CONCLUSION: It is concluded that propofol can confer postconditioning-like cardioprotection in vitro and that scavenging of peroxynitrite during early reperfusion may represent an important mechanism by which propofol postconditioning confers cardioprotection.
 
dc.description.naturelink_to_OA_fulltext
 
dc.descriptionTopic: Experimental Circulation: abstract no. A537
 
dc.identifier.citationThe 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011. In Abstract Archive of the American Society of Anesthesiologists, 2011, abstract no. A537 [How to Cite?]
 
dc.identifier.hkuros209799
 
dc.identifier.urihttp://hdl.handle.net/10722/165919
 
dc.languageeng
 
dc.publisherAmerican Society of Anesthesiologists. The Abstract Archive is located at http://www.asaabstracts.com/strands/asaabstracts/abstractArchive.htm
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAbstract Archive of the American Society of Anesthesiologists
 
dc.titlePropofol confers postconditioning-like cardioprotection via its peroxynitrite scavenging property
 
dc.typeConference_Paper
 
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<item><contributor.author>Mao, X</contributor.author>
<contributor.author>Wong, G</contributor.author>
<contributor.author>Huang, Z</contributor.author>
<contributor.author>Irwin, MG</contributor.author>
<contributor.author>Xia, Z</contributor.author>
<date.accessioned>2012-09-20T08:25:13Z</date.accessioned>
<date.available>2012-09-20T08:25:13Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>The 2011 Annual Meeting of the American Society of Anesthesiologists (ASA 2011), Chicago, IL., 15-19 October 2011. In Abstract Archive of the American Society of Anesthesiologists, 2011, abstract no. A537</identifier.citation>
<identifier.uri>http://hdl.handle.net/10722/165919</identifier.uri>
<description>Topic: Experimental Circulation: abstract no. A537</description>
<description.abstract>BACKGROUND: Propofol, an intravenous anesthetic that possesses antioxidant and peroxynitrite-scavenging properties, has been shown to confer cardioprotection in both experimental and clinical settings, but the precise mechanism is unclear. Reduction of peroxynitrite production (1) has been shown to play a critical role in hypoxia postconditioning mediated cardioprotection in cultured cardiomyocytes. We, therefore, hypothesize that propofol can confer postconditioning (PPC) cardioprotection via its peroxynitrite scavenging property. METHODS: With ethics approval, isolated and Langendorff-perfused hearts from male Sprague Dawley rats were exposed to 30 min of global ischemia followed by 120 min of reperfusion. During the first 15 min of reperfusion, hearts (n=6-8 per group) were either untreated (control group), or respectively treated with 50 &#181;mol/L of propofol (PPC group), 0.1mmol/ 3-morpholino sydnonimine (SIN-1, a peroxynitrite donor which produces nitric oxide and superoxide anion simultaneously in aqueous solution, SIN group), 20 mg/L of the peroxynitrite decomposer FeTPPs (FeTPPs group), the combinations of propofol with SIN-1 (PPC+SIN) or with FeTPPs (PPC+FeTPPs groups). Myocardial infarct size (IS) was assessed by triphenyltetrazolium staining. Data are expressed as mean &#177; SD and were analyzed with ANOVA. RESULTS: Postischemic myocardial infarct size (IS) was 36.7&#177;4.7% in the control group. Propofol postconditioning (PPC) significantly reduced IS (28.1&#177;7.0%, P&lt;0.05 vs. control). However, the infarct-sparing effect of PPC was cancelled by co-administration of SIN-1 (IS: 34.5 &#177;3.9% in PPC+SIN group, P&gt;0.1 vs. control) but facilitated by co-administration of FeTPPs (IS: 15.1&#177;3.8% in PPC+FeTPPs groups, P&lt;0.01 vs. PPC). SIN-1 when given alone during early reperfusion exacerbated postischemic IS (50.9&#177;1.1%, P&lt;0.05 vs. control), while FeTPPs attenuated myocardial IS (21.5 &#177;8.6%, P&lt;0.01 vs. control). CONCLUSION: It is concluded that propofol can confer postconditioning-like cardioprotection in vitro and that scavenging of peroxynitrite during early reperfusion may represent an important mechanism by which propofol postconditioning confers cardioprotection.</description.abstract>
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