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Article: Evaluation of serological tests for H5N1 avian influenza on field samples from domestic poultry populations in Vietnam: consequences for surveillance

TitleEvaluation of serological tests for H5N1 avian influenza on field samples from domestic poultry populations in Vietnam: consequences for surveillance
Authors
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmic
Citation
Veterinary Microbiology, 2012, v. 156 n. 3-4, p. 277-284 How to Cite?
AbstractIn Vietnam, serological post H5N1 vaccination surveillance using the HI test is applied to assess the efficiency of the vaccination in addition to virological monitoring. In this paper we report on the evaluations of the performances of the haemagglutination inhibition (HI) test and of a H5-ELISA, using chicken and duck field samples. The evaluations were conducted by comparison with a pseudotyped-based virus neutralization test (H5pp VNT) performed in a reference laboratory and considered as a 'gold standard' and also by using methods developed for imperfect reference test. Their global accuracy and best cut-offs were also estimated. Results from the HI test for several haemagglutinin subtypes and from a commercial type A influenza competition ELISA were also compared. The results showed that performance of the HI test was very good in comparison with the H5pp VNT. Data also clearly supported the cut-off of >/= 4 log(2) used for the HI test for chickens but, a 3 log(2) positivity cut-off would be more appropriate for ducks. When compared with the VNT, the H5-ELISA showed poor specificity when using the positivity cut-off specified by the manufacturer but could be used as a screening test if confirmed by the HI test or the H5ppVNT which presents some interests for large scale testing (no need for biosafety level 3 conditions and high performance). A general and highly sensitive pre-screening can also be achieved using the detection of NP-specific antibodies with a competition ELISA. This appears of little interest in a context of high subtypes diversity where only a subtype is targeted for surveillance and control.
Persistent Identifierhttp://hdl.handle.net/10722/165793
ISSN
2015 Impact Factor: 2.564
2015 SCImago Journal Rankings: 1.381
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDesvaux, Sen_HK
dc.contributor.authorGarcia, JMen_HK
dc.contributor.authorNguyen, TDen_HK
dc.contributor.authorReid, SAen_HK
dc.contributor.authorBui, NAen_HK
dc.contributor.authorRoger, Fen_HK
dc.contributor.authorFenwick, Sen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorEllis, Ten_HK
dc.date.accessioned2012-09-20T08:23:47Z-
dc.date.available2012-09-20T08:23:47Z-
dc.date.issued2012en_HK
dc.identifier.citationVeterinary Microbiology, 2012, v. 156 n. 3-4, p. 277-284en_HK
dc.identifier.issn0378-1135en_HK
dc.identifier.urihttp://hdl.handle.net/10722/165793-
dc.description.abstractIn Vietnam, serological post H5N1 vaccination surveillance using the HI test is applied to assess the efficiency of the vaccination in addition to virological monitoring. In this paper we report on the evaluations of the performances of the haemagglutination inhibition (HI) test and of a H5-ELISA, using chicken and duck field samples. The evaluations were conducted by comparison with a pseudotyped-based virus neutralization test (H5pp VNT) performed in a reference laboratory and considered as a 'gold standard' and also by using methods developed for imperfect reference test. Their global accuracy and best cut-offs were also estimated. Results from the HI test for several haemagglutinin subtypes and from a commercial type A influenza competition ELISA were also compared. The results showed that performance of the HI test was very good in comparison with the H5pp VNT. Data also clearly supported the cut-off of >/= 4 log(2) used for the HI test for chickens but, a 3 log(2) positivity cut-off would be more appropriate for ducks. When compared with the VNT, the H5-ELISA showed poor specificity when using the positivity cut-off specified by the manufacturer but could be used as a screening test if confirmed by the HI test or the H5ppVNT which presents some interests for large scale testing (no need for biosafety level 3 conditions and high performance). A general and highly sensitive pre-screening can also be achieved using the detection of NP-specific antibodies with a competition ELISA. This appears of little interest in a context of high subtypes diversity where only a subtype is targeted for surveillance and control.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/vetmicen_HK
dc.relation.ispartofVeterinary Microbiologyen_HK
dc.subject.meshChickens - virologyen_HK
dc.subject.meshDucks - virologyen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assay - veterinaryen_HK
dc.subject.meshHemagglutination Inhibition Tests - veterinaryen_HK
dc.subject.meshInfluenza A Virus, H5N1 Subtype - immunology - isolation and purificationen_HK
dc.titleEvaluation of serological tests for H5N1 avian influenza on field samples from domestic poultry populations in Vietnam: consequences for surveillanceen_HK
dc.typeArticleen_HK
dc.identifier.emailDesvaux, S: stephanie.desvaux@cirad.fren_HK
dc.identifier.emailGarcia, JM: jmgarcia@hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.vetmic.2011.11.010en_HK
dc.identifier.pmid22221382-
dc.identifier.scopuseid_2-s2.0-84858748858en_HK
dc.identifier.hkuros205821en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84858748858&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume156en_HK
dc.identifier.issue3-4en_HK
dc.identifier.spage277en_HK
dc.identifier.epage284en_HK
dc.identifier.isiWOS:000302890700006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridEllis, T=7202589926en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridFenwick, S=7004595320en_HK
dc.identifier.scopusauthoridRoger, F=7006292417en_HK
dc.identifier.scopusauthoridBui, NA=35919132500en_HK
dc.identifier.scopusauthoridReid, SA=7201777637en_HK
dc.identifier.scopusauthoridNguyen, TD=25227791100en_HK
dc.identifier.scopusauthoridGarcia, JM=16202601400en_HK
dc.identifier.scopusauthoridDesvaux, S=20435293000en_HK
dc.identifier.citeulike10072788-

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