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Conference Paper: Investigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injury

TitleInvestigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injury
Authors
Issue Date2012
Citation
The 8th FENS Forum of Neuroscience, Barcelona, Spain, 14-18 July 2012. In FENS Abstract, v. 6, abstract no. p061.1 How to Cite?
AbstractNerve/glial antigen 2 (NG2) protein is a single membrane-spanning proteoglycan that is expressed by pericytes, polydendrocytes and some microglia/macrophage (Iba1+/NG2+) in central nervous system. Non-vascular NG2 expressing cells (polydendrocytes & Iba1+/NG2+ cells) in the brain are shown to be multi-potential and have been implicated in neurodegenerative diseases. However, existence and function of possible non-vascular NG2 expressing cells in ocular diseases has not been reported. In the present study, we performed immunocytochemical analysis and identified non-vascular NG2+ cell type in a mouse glaucomatous model characterised with degeneration of retinal ganglion cells (RGCs) induced by acute ocular hypertension. Male C57BL/6N mice aged 6-10 weeks were exposed to acute ocular hypertension by increasing ocular pressure for 1 hour followed by reperfusion for 1 day leading to retinal ischemia/reperfusion (I/R) injury. In normal retina, NG2 expression was restricted to pericytes around blood vessels. However, after injury, we observed some non-vascular NG2 expressing cells away from blood vessels in brain parachyma, and some with ameboid appearance showed close association with RGCs. Interestingly, majority of non-vascular NG2+ cells also expressed Iba-1 (Iba1+/NG2+), which were only found after retinal I/R injury. The source and the role of the non-vascular NG2 expressing cells with respect to injured RGCs are now being under investigation.
DescriptionSession - P061: Ischemia I
Poster Board Number: C151
Abstract no.: 2811
Persistent Identifierhttp://hdl.handle.net/10722/165041

 

DC FieldValueLanguage
dc.contributor.authorFeng, Qen_US
dc.contributor.authorChung, SKen_US
dc.contributor.authorSo, KFen_US
dc.date.accessioned2012-09-20T08:13:49Z-
dc.date.available2012-09-20T08:13:49Z-
dc.date.issued2012en_US
dc.identifier.citationThe 8th FENS Forum of Neuroscience, Barcelona, Spain, 14-18 July 2012. In FENS Abstract, v. 6, abstract no. p061.1en_US
dc.identifier.urihttp://hdl.handle.net/10722/165041-
dc.descriptionSession - P061: Ischemia I-
dc.descriptionPoster Board Number: C151-
dc.descriptionAbstract no.: 2811-
dc.description.abstractNerve/glial antigen 2 (NG2) protein is a single membrane-spanning proteoglycan that is expressed by pericytes, polydendrocytes and some microglia/macrophage (Iba1+/NG2+) in central nervous system. Non-vascular NG2 expressing cells (polydendrocytes & Iba1+/NG2+ cells) in the brain are shown to be multi-potential and have been implicated in neurodegenerative diseases. However, existence and function of possible non-vascular NG2 expressing cells in ocular diseases has not been reported. In the present study, we performed immunocytochemical analysis and identified non-vascular NG2+ cell type in a mouse glaucomatous model characterised with degeneration of retinal ganglion cells (RGCs) induced by acute ocular hypertension. Male C57BL/6N mice aged 6-10 weeks were exposed to acute ocular hypertension by increasing ocular pressure for 1 hour followed by reperfusion for 1 day leading to retinal ischemia/reperfusion (I/R) injury. In normal retina, NG2 expression was restricted to pericytes around blood vessels. However, after injury, we observed some non-vascular NG2 expressing cells away from blood vessels in brain parachyma, and some with ameboid appearance showed close association with RGCs. Interestingly, majority of non-vascular NG2+ cells also expressed Iba-1 (Iba1+/NG2+), which were only found after retinal I/R injury. The source and the role of the non-vascular NG2 expressing cells with respect to injured RGCs are now being under investigation.-
dc.languageengen_US
dc.relation.ispartofFENS Abstracten_US
dc.titleInvestigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injuryen_US
dc.typeConference_Paperen_US
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_US
dc.identifier.emailSo, KF: hrmaskf@hku.hken_US
dc.identifier.authorityChung, SK=rp00381en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros211347en_US
dc.identifier.volume6-

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