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Conference Paper: Investigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injury

TitleInvestigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injury
Authors
Issue Date2012
Citation
The 8th Forum of Neuroscience of the Federation of European Neuroscience Societies (FENS 2012). In FENS Abstract, 2012, v. 6, abstract no. p061.1 How to Cite?
AbstractNerve/glial antigen 2 (NG2) protein is a single membrane-spanning proteoglycan that is expressed by pericytes, polydendrocytes and some microglia/macrophage (Iba1+/NG2+) in central nervous system. Non-vascular NG2 expressing cells (polydendrocytes & Iba1+/NG2+ cells) in the brain are shown to be multi-potential and have been implicated in neurodegenerative diseases. However, existence and function of possible non-vascular NG2 expressing cells in ocular diseases has not been reported. In the present study, we performed immunocytochemical analysis and identified non-vascular NG2+ cell type in a mouse glaucomatous model characterised with degeneration of retinal ganglion cells (RGCs) induced by acute ocular hypertension. Male C57BL/6N mice aged 6-10 weeks were exposed to acute ocular hypertension by increasing ocular pressure for 1 hour followed by reperfusion for 1 day leading to retinal ischemia/reperfusion (I/R) injury. In normal retina, NG2 expression was restricted to pericytes around blood vessels. However, after injury, we observed some non-vascular NG2 expressing cells away from blood vessels in brain parachyma, and some with ameboid appearance showed close association with RGCs. Interestingly, majority of non-vascular NG2+ cells also expressed Iba-1 (Iba1+/NG2+), which were only found after retinal I/R injury. The source and the role of the non-vascular NG2 expressing cells with respect to injured RGCs are now being under investigation.
DescriptionSession - P061: Ischemia 1: Poster Board Number: C151: abstract no.: 2811
Persistent Identifierhttp://hdl.handle.net/10722/165041

 

DC FieldValueLanguage
dc.contributor.authorFeng, Qen_US
dc.contributor.authorChung, SKen_US
dc.contributor.authorSo, KFen_US
dc.date.accessioned2012-09-20T08:13:49Z-
dc.date.available2012-09-20T08:13:49Z-
dc.date.issued2012en_US
dc.identifier.citationThe 8th Forum of Neuroscience of the Federation of European Neuroscience Societies (FENS 2012). In FENS Abstract, 2012, v. 6, abstract no. p061.1en_US
dc.identifier.urihttp://hdl.handle.net/10722/165041-
dc.descriptionSession - P061: Ischemia 1: Poster Board Number: C151: abstract no.: 2811-
dc.description.abstractNerve/glial antigen 2 (NG2) protein is a single membrane-spanning proteoglycan that is expressed by pericytes, polydendrocytes and some microglia/macrophage (Iba1+/NG2+) in central nervous system. Non-vascular NG2 expressing cells (polydendrocytes & Iba1+/NG2+ cells) in the brain are shown to be multi-potential and have been implicated in neurodegenerative diseases. However, existence and function of possible non-vascular NG2 expressing cells in ocular diseases has not been reported. In the present study, we performed immunocytochemical analysis and identified non-vascular NG2+ cell type in a mouse glaucomatous model characterised with degeneration of retinal ganglion cells (RGCs) induced by acute ocular hypertension. Male C57BL/6N mice aged 6-10 weeks were exposed to acute ocular hypertension by increasing ocular pressure for 1 hour followed by reperfusion for 1 day leading to retinal ischemia/reperfusion (I/R) injury. In normal retina, NG2 expression was restricted to pericytes around blood vessels. However, after injury, we observed some non-vascular NG2 expressing cells away from blood vessels in brain parachyma, and some with ameboid appearance showed close association with RGCs. Interestingly, majority of non-vascular NG2+ cells also expressed Iba-1 (Iba1+/NG2+), which were only found after retinal I/R injury. The source and the role of the non-vascular NG2 expressing cells with respect to injured RGCs are now being under investigation.-
dc.languageengen_US
dc.relation.ispartofFENS Abstracten_US
dc.titleInvestigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injuryen_US
dc.typeConference_Paperen_US
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_US
dc.identifier.emailSo, KF: hrmaskf@hku.hken_US
dc.identifier.authorityChung, SK=rp00381en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros211347en_US
dc.identifier.volume6, abstract no. p061.1-

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