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Conference Paper: Investigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injury
Title | Investigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injury |
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Authors | |
Issue Date | 2012 |
Citation | The 8th Forum of Neuroscience of the Federation of European Neuroscience Societies (FENS 2012). In FENS Abstract, 2012, v. 6, abstract no. p061.1 How to Cite? |
Abstract | Nerve/glial antigen 2 (NG2) protein is a single membrane-spanning proteoglycan that is expressed by pericytes, polydendrocytes and some microglia/macrophage (Iba1+/NG2+) in central nervous system. Non-vascular NG2 expressing cells (polydendrocytes & Iba1+/NG2+ cells) in the brain are shown to be multi-potential and have been implicated in neurodegenerative diseases. However, existence and function of possible non-vascular NG2 expressing cells in ocular diseases has not been reported. In the present study, we performed immunocytochemical analysis and identified non-vascular NG2+ cell type in a mouse glaucomatous model characterised with degeneration of retinal ganglion cells (RGCs) induced by acute ocular hypertension. Male C57BL/6N mice aged 6-10 weeks were exposed to acute ocular hypertension by increasing ocular pressure for 1 hour followed by reperfusion for 1 day leading to retinal ischemia/reperfusion (I/R) injury. In normal retina, NG2 expression was restricted to pericytes around blood vessels. However, after injury, we observed some non-vascular NG2 expressing cells away from blood vessels in brain parachyma, and some with ameboid appearance showed close association with RGCs. Interestingly, majority of non-vascular NG2+ cells also expressed Iba-1 (Iba1+/NG2+), which were only found after retinal I/R injury. The source and the role of the non-vascular NG2 expressing cells with respect to injured RGCs are now being under investigation. |
Description | Session - P061: Ischemia 1: Poster Board Number: C151: abstract no.: 2811 |
Persistent Identifier | http://hdl.handle.net/10722/165041 |
DC Field | Value | Language |
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dc.contributor.author | Feng, Q | en_US |
dc.contributor.author | Chung, SK | en_US |
dc.contributor.author | So, KF | en_US |
dc.date.accessioned | 2012-09-20T08:13:49Z | - |
dc.date.available | 2012-09-20T08:13:49Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | The 8th Forum of Neuroscience of the Federation of European Neuroscience Societies (FENS 2012). In FENS Abstract, 2012, v. 6, abstract no. p061.1 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/165041 | - |
dc.description | Session - P061: Ischemia 1: Poster Board Number: C151: abstract no.: 2811 | - |
dc.description.abstract | Nerve/glial antigen 2 (NG2) protein is a single membrane-spanning proteoglycan that is expressed by pericytes, polydendrocytes and some microglia/macrophage (Iba1+/NG2+) in central nervous system. Non-vascular NG2 expressing cells (polydendrocytes & Iba1+/NG2+ cells) in the brain are shown to be multi-potential and have been implicated in neurodegenerative diseases. However, existence and function of possible non-vascular NG2 expressing cells in ocular diseases has not been reported. In the present study, we performed immunocytochemical analysis and identified non-vascular NG2+ cell type in a mouse glaucomatous model characterised with degeneration of retinal ganglion cells (RGCs) induced by acute ocular hypertension. Male C57BL/6N mice aged 6-10 weeks were exposed to acute ocular hypertension by increasing ocular pressure for 1 hour followed by reperfusion for 1 day leading to retinal ischemia/reperfusion (I/R) injury. In normal retina, NG2 expression was restricted to pericytes around blood vessels. However, after injury, we observed some non-vascular NG2 expressing cells away from blood vessels in brain parachyma, and some with ameboid appearance showed close association with RGCs. Interestingly, majority of non-vascular NG2+ cells also expressed Iba-1 (Iba1+/NG2+), which were only found after retinal I/R injury. The source and the role of the non-vascular NG2 expressing cells with respect to injured RGCs are now being under investigation. | - |
dc.language | eng | en_US |
dc.relation.ispartof | FENS Abstract | en_US |
dc.title | Investigation of non-vascular NG2 expressing cells in the adult mouse retina after ischemia-reperfusion injury | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Chung, SK: skchung@hkucc.hku.hk | en_US |
dc.identifier.email | So, KF: hrmaskf@hku.hk | en_US |
dc.identifier.authority | Chung, SK=rp00381 | en_US |
dc.identifier.hkuros | 211347 | en_US |
dc.identifier.volume | 6, abstract no. p061.1 | - |