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Conference Paper: Reduction of contractions to phenylephrine by L-NAME in the carotid artery of mice with endothelial overexpression of endothelin-1
Title | Reduction of contractions to phenylephrine by L-NAME in the carotid artery of mice with endothelial overexpression of endothelin-1 |
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Authors | |
Keywords | Biology |
Issue Date | 2012 |
Publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ |
Citation | The 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 1129.5 How to Cite? |
Abstract | The release of endothelium-derived contracting factors (EDCF) is increased by exogenous endothelin-1 (ET-1). Experiments were designed to determine whether or not endothelial overexpression of ET-1 affects vascular responsiveness. Carotid artery rings with endothelium of control mice and mice with endothelial overexpression of ET-1 (TET-1) were contracted with the α1-adrenoceptor agonist phenylephrine in the absence or presence of the eNOS inhibitor L-NAME and exposed to acetylcholine. After washout, the rings were contracted with increasing concentrations of the TP receptor agonist U46619. EDCF-mediated increases in tension caused by acetylcholine were augmented in TET-1 arteries compared to controls both in the absence and presence of LNAME. In the presence of the latter, TET-1 rings showed stronger contractions to U46619 than controls. By contrast, contractions to phenylephrine were reduced by L-NAME in TET-1 arteries with a rightward shift and loss of the sigmoidal shape of the concentration-response curve. These results imply a role of endothelial ET-1 in EDCF-mediated contractions and indicate an increased TXA2 receptor signaling in vascular smooth muscle cells. They do not provide an explanation for the decreased response to α1-adrenergic activation in TET-1 arteries in the absence of NO. |
Description | Session - 1129. Endothelial Cell Biology - Poster: no. 1129.5 |
Persistent Identifier | http://hdl.handle.net/10722/165037 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.412 |
DC Field | Value | Language |
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dc.contributor.author | Baretella, OM | en_US |
dc.contributor.author | Li, FYL | en_US |
dc.contributor.author | Chung, SK | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.date.accessioned | 2012-09-20T08:13:46Z | - |
dc.date.available | 2012-09-20T08:13:46Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | The 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 1129.5 | en_US |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10722/165037 | - |
dc.description | Session - 1129. Endothelial Cell Biology - Poster: no. 1129.5 | - |
dc.description.abstract | The release of endothelium-derived contracting factors (EDCF) is increased by exogenous endothelin-1 (ET-1). Experiments were designed to determine whether or not endothelial overexpression of ET-1 affects vascular responsiveness. Carotid artery rings with endothelium of control mice and mice with endothelial overexpression of ET-1 (TET-1) were contracted with the α1-adrenoceptor agonist phenylephrine in the absence or presence of the eNOS inhibitor L-NAME and exposed to acetylcholine. After washout, the rings were contracted with increasing concentrations of the TP receptor agonist U46619. EDCF-mediated increases in tension caused by acetylcholine were augmented in TET-1 arteries compared to controls both in the absence and presence of LNAME. In the presence of the latter, TET-1 rings showed stronger contractions to U46619 than controls. By contrast, contractions to phenylephrine were reduced by L-NAME in TET-1 arteries with a rightward shift and loss of the sigmoidal shape of the concentration-response curve. These results imply a role of endothelial ET-1 in EDCF-mediated contractions and indicate an increased TXA2 receptor signaling in vascular smooth muscle cells. They do not provide an explanation for the decreased response to α1-adrenergic activation in TET-1 arteries in the absence of NO. | - |
dc.language | eng | en_US |
dc.publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ | - |
dc.relation.ispartof | The FASEB Journal | en_US |
dc.subject | Biology | - |
dc.title | Reduction of contractions to phenylephrine by L-NAME in the carotid artery of mice with endothelial overexpression of endothelin-1 | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Chung, SK: skchung@hkucc.hku.hk | en_US |
dc.identifier.email | Vanhoutte, PM: vanhoutt@hku.hk | en_US |
dc.identifier.authority | Chung, SK=rp00381 | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.hkuros | 210679 | en_US |
dc.identifier.volume | 26 | - |
dc.identifier.issue | meeting abstract | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0892-6638 | - |