File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Biofilm Growth Characteristics of Candida on Central Venous Catheters

TitleBiofilm Growth Characteristics of Candida on Central Venous Catheters
Authors
KeywordsAntimicrobials
Biofilm
Fungi
Infection and Serum-plasma
Issue Date2012
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 90th General Session & Exhibition of the International Association for Dental Research (IADR), Iguacu Falls, Brazil, 20-23 June 2012. In Journal of Dental Research, 2012, v. 91 n. Special issue B: abstract no. 1549 How to Cite?
AbstractC. albicans is frequently isolated from blood stream infections associated with central venous catheters. Objectives: a) To fully characterize the growth and biofilm development of C. albicans on central venous catheter surfaces (CVCs) in YNB medium, b) to determine the expression of upregulated biofilm specific genes encoding adhesins and hyphae in Candida biofilms grown on serum coated/ uncoated catheters and, c) to evaluate the antimycotic efficacy of amphotericin B, azole antifungals and the combination of polyene and azole drugs for the prevention of Candida biofilm growth on catheter surfaces. Methods: Biofilm growth of C. albicans on CVCs in Yeast nitrogen base medium during varying time intervals and growth conditions were determined using the tetrazolium reduction assay. Scanning electron microscopy and Confocal scanning laser microscopy were used to further characterize Candida biofilms. The differential mRNA transcript levels of biofilm associated genes were determined by quantitative real-time PCR analysis. Results: 1) Biofilm growth was significantly (p<0.001) higher in serum coated CVCs and consisted of yeast and hyphal elements embedded with extracellular material for all C. albicans strains, 2) A significant upregulation of the adhesin genes ALS3, HWP1, EAP1 and the overexpression of ECE1 hyphal gene was observed for clinical C. albicans biofilms (for at least one time point) grown on CVCs in the presence of serum whereas all the above described genes were underexpressed in C. albicans biofilms grown on serum-free controls, 3) Antifungal susceptibility tests demonstrated that (×3MIC) amphotericin B, (×24MIC) Itraconazole and (×12MIC) ketoconazole and fluconazole killed approximately 50% of 48h C. albicans biofilms on serum coated CVCs. Furthermore amphotericin B in association with ketoconazole exhibited effective synergistic killing of biofilm Candida compared with the combined effect of amphotericin B with either itraconazole or fluconazole. Conclusion: Serum noticeably enhances the upregulation of genes crucial for biofilm development.
DescriptionPoster Presentation
Session 168: Candida II
Persistent Identifierhttp://hdl.handle.net/10722/164983
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_US
dc.contributor.authorCheung, BPKen_US
dc.contributor.authorYau, JYYen_US
dc.contributor.authorYeung, SKWen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2012-09-20T08:13:14Z-
dc.date.available2012-09-20T08:13:14Z-
dc.date.issued2012en_US
dc.identifier.citationThe 90th General Session & Exhibition of the International Association for Dental Research (IADR), Iguacu Falls, Brazil, 20-23 June 2012. In Journal of Dental Research, 2012, v. 91 n. Special issue B: abstract no. 1549en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/164983-
dc.descriptionPoster Presentation-
dc.descriptionSession 168: Candida II-
dc.description.abstractC. albicans is frequently isolated from blood stream infections associated with central venous catheters. Objectives: a) To fully characterize the growth and biofilm development of C. albicans on central venous catheter surfaces (CVCs) in YNB medium, b) to determine the expression of upregulated biofilm specific genes encoding adhesins and hyphae in Candida biofilms grown on serum coated/ uncoated catheters and, c) to evaluate the antimycotic efficacy of amphotericin B, azole antifungals and the combination of polyene and azole drugs for the prevention of Candida biofilm growth on catheter surfaces. Methods: Biofilm growth of C. albicans on CVCs in Yeast nitrogen base medium during varying time intervals and growth conditions were determined using the tetrazolium reduction assay. Scanning electron microscopy and Confocal scanning laser microscopy were used to further characterize Candida biofilms. The differential mRNA transcript levels of biofilm associated genes were determined by quantitative real-time PCR analysis. Results: 1) Biofilm growth was significantly (p<0.001) higher in serum coated CVCs and consisted of yeast and hyphal elements embedded with extracellular material for all C. albicans strains, 2) A significant upregulation of the adhesin genes ALS3, HWP1, EAP1 and the overexpression of ECE1 hyphal gene was observed for clinical C. albicans biofilms (for at least one time point) grown on CVCs in the presence of serum whereas all the above described genes were underexpressed in C. albicans biofilms grown on serum-free controls, 3) Antifungal susceptibility tests demonstrated that (×3MIC) amphotericin B, (×24MIC) Itraconazole and (×12MIC) ketoconazole and fluconazole killed approximately 50% of 48h C. albicans biofilms on serum coated CVCs. Furthermore amphotericin B in association with ketoconazole exhibited effective synergistic killing of biofilm Candida compared with the combined effect of amphotericin B with either itraconazole or fluconazole. Conclusion: Serum noticeably enhances the upregulation of genes crucial for biofilm development.-
dc.languageengen_US
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc..-
dc.subjectAntimicrobials-
dc.subjectBiofilm-
dc.subjectFungi-
dc.subjectInfection and Serum-plasma-
dc.titleBiofilm Growth Characteristics of Candida on Central Venous Cathetersen_US
dc.typeConference_Paperen_US
dc.identifier.emailSamaranayake, YH: hema@hkucc.hku.hken_US
dc.identifier.emailCheung, BPK: bpkcheun@hkucc.hku.hken_US
dc.identifier.emailYau, JYY: yaujyy@hkucc.hku.hken_US
dc.identifier.emailYeung, SKW: skwyeung@hku.hken_US
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_US
dc.identifier.authoritySamaranayake, YH=rp00025en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.identifier.hkuros208782en_US
dc.identifier.volume91en_US
dc.identifier.issueSpecial issue B: abstract no. 1549-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats