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Article: Role of label-retaining cells in estrogen-induced endometrial regeneration
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TitleRole of label-retaining cells in estrogen-induced endometrial regeneration
 
AuthorsChan, RWS2 3
Kaitu'u-Lino, T1 3
Gargett, CE1 3
 
Issue Date2012
 
PublisherSage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.com
 
CitationReproductive Sciences, 2012, v. 19 n. 1, p. 102-114 [How to Cite?]
DOI: http://dx.doi.org/10.1177/1933719111414207
 
AbstractCandidate stem/progenitor cells have been identified in mouse endometrium as label-retaining cells (LRCs). The role of endometrial stem/progenitor cells in initiating estrogen-stimulated endometrial growth in prepubertal and cycling mice was investigated following a single 17beta-estradiol (E2) injection in bromodeoxyuridine (BrdU)-labeled and -chased (LRC), ovariectomised mice. Proliferating (BrdU(+)/Ki-67(+)) and mitotic (BrdU(+)/PH3(+)) epithelial LRCs were first detected in prepubertal mice 8 hours following E2 treatment, initiating the proliferative response. In contrast, all epithelial LRCs and 16% of epithelial cells in cycling mice proliferated within 2 hours. In cycling mice, 12% of stromal LRCs initiated a proliferative response 8 hours after E2. Proliferating epithelial LRCs and most stromal LRCs (85%) lacked estrogen receptor-alpha (ESR1). These findings suggest that endometrial epithelial LRCs function as stem/progenitor cells by receiving proliferative signals from neighboring ESR1(+) niche cells to initiate the growth of the epithelium during development, while mature epithelial cells may undergo self-replication in cycling endometrium.
 
ISSN1933-7191
2012 Impact Factor: 2.064
2012 SCImago Journal Rankings: 0.761
 
DOIhttp://dx.doi.org/10.1177/1933719111414207
 
DC FieldValue
dc.contributor.authorChan, RWS
 
dc.contributor.authorKaitu'u-Lino, T
 
dc.contributor.authorGargett, CE
 
dc.date.accessioned2012-09-20T08:10:04Z
 
dc.date.available2012-09-20T08:10:04Z
 
dc.date.issued2012
 
dc.description.abstractCandidate stem/progenitor cells have been identified in mouse endometrium as label-retaining cells (LRCs). The role of endometrial stem/progenitor cells in initiating estrogen-stimulated endometrial growth in prepubertal and cycling mice was investigated following a single 17beta-estradiol (E2) injection in bromodeoxyuridine (BrdU)-labeled and -chased (LRC), ovariectomised mice. Proliferating (BrdU(+)/Ki-67(+)) and mitotic (BrdU(+)/PH3(+)) epithelial LRCs were first detected in prepubertal mice 8 hours following E2 treatment, initiating the proliferative response. In contrast, all epithelial LRCs and 16% of epithelial cells in cycling mice proliferated within 2 hours. In cycling mice, 12% of stromal LRCs initiated a proliferative response 8 hours after E2. Proliferating epithelial LRCs and most stromal LRCs (85%) lacked estrogen receptor-alpha (ESR1). These findings suggest that endometrial epithelial LRCs function as stem/progenitor cells by receiving proliferative signals from neighboring ESR1(+) niche cells to initiate the growth of the epithelium during development, while mature epithelial cells may undergo self-replication in cycling endometrium.
 
dc.description.naturepostprint
 
dc.identifier.citationReproductive Sciences, 2012, v. 19 n. 1, p. 102-114 [How to Cite?]
DOI: http://dx.doi.org/10.1177/1933719111414207
 
dc.identifier.doihttp://dx.doi.org/10.1177/1933719111414207
 
dc.identifier.epage114
 
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dc.identifier.hkuros206846
 
dc.identifier.issn1933-7191
2012 Impact Factor: 2.064
2012 SCImago Journal Rankings: 0.761
 
dc.identifier.issue1
 
dc.identifier.pmid22064386
 
dc.identifier.scopuseid_2-s2.0-84855501315
 
dc.identifier.spage102
 
dc.identifier.urihttp://hdl.handle.net/10722/164809
 
dc.identifier.volume19
 
dc.languageeng
 
dc.publisherSage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.com
 
dc.publisher.placeUnited States
 
dc.relation.ispartofReproductive Sciences
 
dc.rightsReproductive Sciences. Copyright © Sage Publications, Inc.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshCell Proliferation - drug effects
 
dc.subject.meshEndometrium - cytology - drug effects
 
dc.subject.meshEstrogens - pharmacology
 
dc.subject.meshRegeneration - drug effects
 
dc.subject.meshStem Cells - drug effects
 
dc.titleRole of label-retaining cells in estrogen-induced endometrial regeneration
 
dc.typeArticle
 
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Author Affiliations
  1. Monash Institute of Medical Research
  2. The University of Hong Kong
  3. Monash University