Article: Role of label-retaining cells in estrogen-induced endometrial regeneration

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Publisher Website: 10.1177/1933719111414207
Scopus: eid_2-s2.0-84855501315
PMID: 22064386

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Title	Role of label-retaining cells in estrogen-induced endometrial regeneration
Authors	Chan, RWS2 3 Kaitu'u-Lino, T1 3 Gargett, CE1 3
Issue Date	2012
Publisher	Sage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.com
Citation	Reproductive Sciences, 2012, v. 19 n. 1, p. 102-114 [How to Cite?] DOI: http://dx.doi.org/10.1177/1933719111414207
Abstract	Candidate stem/progenitor cells have been identified in mouse endometrium as label-retaining cells (LRCs). The role of endometrial stem/progenitor cells in initiating estrogen-stimulated endometrial growth in prepubertal and cycling mice was investigated following a single 17beta-estradiol (E2) injection in bromodeoxyuridine (BrdU)-labeled and -chased (LRC), ovariectomised mice. Proliferating (BrdU(+)/Ki-67(+)) and mitotic (BrdU(+)/PH3(+)) epithelial LRCs were first detected in prepubertal mice 8 hours following E2 treatment, initiating the proliferative response. In contrast, all epithelial LRCs and 16% of epithelial cells in cycling mice proliferated within 2 hours. In cycling mice, 12% of stromal LRCs initiated a proliferative response 8 hours after E2. Proliferating epithelial LRCs and most stromal LRCs (85%) lacked estrogen receptor-alpha (ESR1). These findings suggest that endometrial epithelial LRCs function as stem/progenitor cells by receiving proliferative signals from neighboring ESR1(+) niche cells to initiate the growth of the epithelium during development, while mature epithelial cells may undergo self-replication in cycling endometrium.
ISSN	1933-7191 2011 Impact Factor: 2.444 2011 SCImago Journal Rankings: 0.180
DOI	http://dx.doi.org/10.1177/1933719111414207

DC Field	Value
dc.contributor.author	Chan, RWS
dc.contributor.author	Kaitu'u-Lino, T
dc.contributor.author	Gargett, CE
dc.date.accessioned	2012-09-20T08:10:04Z
dc.date.available	2012-09-20T08:10:04Z
dc.date.issued	2012
dc.description.abstract	Candidate stem/progenitor cells have been identified in mouse endometrium as label-retaining cells (LRCs). The role of endometrial stem/progenitor cells in initiating estrogen-stimulated endometrial growth in prepubertal and cycling mice was investigated following a single 17beta-estradiol (E2) injection in bromodeoxyuridine (BrdU)-labeled and -chased (LRC), ovariectomised mice. Proliferating (BrdU(+)/Ki-67(+)) and mitotic (BrdU(+)/PH3(+)) epithelial LRCs were first detected in prepubertal mice 8 hours following E2 treatment, initiating the proliferative response. In contrast, all epithelial LRCs and 16% of epithelial cells in cycling mice proliferated within 2 hours. In cycling mice, 12% of stromal LRCs initiated a proliferative response 8 hours after E2. Proliferating epithelial LRCs and most stromal LRCs (85%) lacked estrogen receptor-alpha (ESR1). These findings suggest that endometrial epithelial LRCs function as stem/progenitor cells by receiving proliferative signals from neighboring ESR1(+) niche cells to initiate the growth of the epithelium during development, while mature epithelial cells may undergo self-replication in cycling endometrium.
dc.description.nature	postprint
dc.identifier.citation	Reproductive Sciences, 2012, v. 19 n. 1, p. 102-114 [How to Cite?] DOI: http://dx.doi.org/10.1177/1933719111414207
dc.identifier.doi	http://dx.doi.org/10.1177/1933719111414207
dc.identifier.epage	114
dc.identifier.hkuros	206846
dc.identifier.issn	1933-7191 2011 Impact Factor: 2.444 2011 SCImago Journal Rankings: 0.180
dc.identifier.issue	1
dc.identifier.pmid	22064386
dc.identifier.scopus	eid_2-s2.0-84855501315
dc.identifier.spage	102
dc.identifier.uri	http://hdl.handle.net/10722/164809
dc.identifier.volume	19
dc.language	eng
dc.publisher	Sage Publications, Inc. The Journal's web site is located at http://rsx.sagepub.com
dc.publisher.place	United States
dc.relation.ispartof	Reproductive Sciences
dc.rights	Reproductive Sciences. Copyright © Sage Publications, Inc.
dc.rights	Creative Commons: Attribution 3.0 Hong Kong License
dc.subject.mesh	Cell Proliferation - drug effects
dc.subject.mesh	Endometrium - cytology - drug effects
dc.subject.mesh	Estrogens - pharmacology
dc.subject.mesh	Regeneration - drug effects
dc.subject.mesh	Stem Cells - drug effects
dc.title	Role of label-retaining cells in estrogen-induced endometrial regeneration
dc.type	Article

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Author Affiliations

Monash Institute of Medical Research
The University of Hong Kong
Monash University