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Article: Titration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaques

TitleTitration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaques
SHIV1157ipd3N4中國恒河猴細胞適應株靜脈感染中國恒河猴有效濃度的確定
Authors
Issue Date2011
Publisher中國實驗動物學會
Citation
中國比較醫學雜誌, 2011, v. 21 n. 4, p. 12-15 How to Cite?
Chinese Journal of Comparative Medicine, 2011, v. 21 n. 4, p. 12-15 How to Cite?
Abstract目的確定SHIV1157ipd3N4靜脈途徑感染中國恒河猴的有效病毒濃度,明確SHIV1157ipd3N4感染實驗猴體內病毒復制和免疫損傷情況。方法 10只正常中國恒河猴分成6組,分別用10倍系列稀釋的病毒液1 mL靜脈感染,測定血漿病毒載量,CD4+/CD8+,CD4+T淋巴細胞絕對數,分析感染后恒河猴體內病毒復制和免疫損傷情況。結果 5TCID50/mL以上濃度的SHIV1157ipd3N4能通過靜脈途徑感染中國恒河猴。結論該實驗的成功進行為SHIV/中國恒河猴疾病及評價模型的建立奠定了良好的基礎,為今后使用此模型評價抗病毒藥物或疫苗提供了條件。
Objective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously, and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation. Methods 10 Chinese-origin rhesus macaques were used in this study. Firstly, all the monkeys were screened by serological test to ensure that they were free of SIV,SRV, and STLV. Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately. Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR. Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i. v. successfully. Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously. This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.
Persistent Identifierhttp://hdl.handle.net/10722/164390
ISSN

 

DC FieldValueLanguage
dc.contributor.authorCong, Zen_US
dc.contributor.authorJiang, Hen_US
dc.contributor.authorJin, Gen_US
dc.contributor.authorChen, Ten_US
dc.contributor.authorWang, Wen_US
dc.contributor.authorTao, Zen_US
dc.contributor.authorYao, Nen_US
dc.contributor.authorXiong, Jen_US
dc.contributor.authorWu, FXen_US
dc.contributor.authorChen, Zen_US
dc.contributor.authorWei, Qen_US
dc.date.accessioned2012-09-20T07:58:50Z-
dc.date.available2012-09-20T07:58:50Z-
dc.date.issued2011en_US
dc.identifier.citation中國比較醫學雜誌, 2011, v. 21 n. 4, p. 12-15en_US
dc.identifier.citationChinese Journal of Comparative Medicine, 2011, v. 21 n. 4, p. 12-15-
dc.identifier.issn1671-7856-
dc.identifier.urihttp://hdl.handle.net/10722/164390-
dc.description.abstract目的確定SHIV1157ipd3N4靜脈途徑感染中國恒河猴的有效病毒濃度,明確SHIV1157ipd3N4感染實驗猴體內病毒復制和免疫損傷情況。方法 10只正常中國恒河猴分成6組,分別用10倍系列稀釋的病毒液1 mL靜脈感染,測定血漿病毒載量,CD4+/CD8+,CD4+T淋巴細胞絕對數,分析感染后恒河猴體內病毒復制和免疫損傷情況。結果 5TCID50/mL以上濃度的SHIV1157ipd3N4能通過靜脈途徑感染中國恒河猴。結論該實驗的成功進行為SHIV/中國恒河猴疾病及評價模型的建立奠定了良好的基礎,為今后使用此模型評價抗病毒藥物或疫苗提供了條件。-
dc.description.abstractObjective To determine the viral concentration of SHIV1157ipd3N4 to infect Chinese-origin rhesus macaques intravenously, and to clarify the viral replication and immunological impairment in the monkeys after SHIV1157ipd3N4 inoculation. Methods 10 Chinese-origin rhesus macaques were used in this study. Firstly, all the monkeys were screened by serological test to ensure that they were free of SIV,SRV, and STLV. Then they were infected with 1 mL of 10-fold serial dilution of SHIV1157ipd3N4 separately. Finally to make blood routine examination and measure the viral loads of the monkeys by real-time RT-PCR. Results More than 5 TCID50 /mL of SHIV1157ipd3N4 can infect the Chinese-origin rhesus macaques i. v. successfully. Conclusions Our findings of this study provide a firm basis for establishing a SHIV1157ipd3N4 infection model of Chinese rhesus macaques intravenously. This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.-
dc.languagechien_US
dc.publisher中國實驗動物學會-
dc.relation.ispartof中國比較醫學雜誌en_US
dc.relation.ispartofChinese Journal of Comparative Medicine-
dc.titleTitration of a SHIV1157ipd3N4 Stock by Intravenous Inoculation of Chinese-Origin Rhesus Macaquesen_US
dc.titleSHIV1157ipd3N4中國恒河猴細胞適應株靜脈感染中國恒河猴有效濃度的確定-
dc.typeArticleen_US
dc.identifier.emailChen, Z: zchenai@hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.identifier.doi10.3969/j.issn.1671.7856.2011.04.003-
dc.identifier.hkuros206286en_US
dc.identifier.volume21en_US
dc.identifier.issue4en_US
dc.identifier.spage12en_US
dc.identifier.epage15en_US
dc.publisher.placeChina-

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