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Article: Effect of CD8+ T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infection

TitleEffect of CD8+ T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infection
體內CD8+T細胞剔除對無癥狀期SHIV感染猴的影
Authors
KeywordsMacaca mulatta (恒河猴)
HIV
T-Lymphocytes
Infection
Viruses
Issue Date2011
Publisher中國實驗動物學會
Citation
中國比較醫學雜誌, 2011, v. 21 n. 4, p. 1-5 How to Cite?
Chinese Journal of Comparative Medicine, 2011, v. 21 n. 4, p. 1-5 How to Cite?
Abstract目的 CD8+T細胞在一些病毒感染疾病的免疫反應中起著重要的作用,但CD8+T細胞在HIV無癥狀期的作用尚不明確,本研究通過體內CD8+T細胞剔除,研究CD8+T細胞對SHIV感染猴的影響,進一步了解艾滋病的發病機制。方法選擇8只SHIV病毒感染的恒河猴,均處于無癥狀期,隨機分成兩組,實驗組4只恒河猴在0、3、7 d注射抗CD8+T抗體cM-T807,不同的時間取外周血、腹股溝淋巴結。流式細胞術測定恒河猴外周血和淋巴結中CD8+T細胞數目,Real-time RT-PCR法測定實驗猴血漿病毒載量,并使用IFN-γElispot方法測定其對猴細胞免疫的影響。結果 CD8+T細胞敲除后,4只猴的病毒載量都轉陽,但反應性不一,HIV-1的靶細胞CD4+T細胞有輕微下降,后反彈,與病毒載量無相關性;CD8敲除猴的感染情況(血漿病毒載量和CD4細胞)比SHIV病毒急性感染輕,這與ELIPOT結果一致。結論 CD8+T細胞在HIV無癥狀期發揮重要的作用,但其作用具有個體差別。
Objective CD8+ T cells play an important role in some viral diseases,but the role of CD8+ T cells is not yet fully clear in the asymptomatic phase of HIV infection.The aim of this study was to elucidate the impact of CD8 + T cell in SHIV-infected monkeys with in vivo CD8 + T cells depletion,and to further understand the pathogenesis of AIDS.Methods Eight SHIV-infected rhesus monkeys in the asymptomatic phase were selected and randomly divided into two groups.Anti-CD8 + T antibody cM-T807 were injected intravenously into four monkeys on days 0,3,and 7.Then the CD8 + T cell number in peripheral blood and lymph nodes in the monkeys were detected by flow cytometry,the plasma viral load by real-time RT-PCR,and the cellular immunity by IFN-γ Elispot assay.Results The viral load of the four monkeys was elevated to above of detected after the CD8 + T cell depletion,but the reactions were not uniform.CD4 + T cells,the HIV-1 target cells,rebound and dropped slightly.The infection of monkeys with CD8 + T cell depletion was lighter than that after the SHIV virus first infection(plasma viral load and CD4 cells),which was consistent with the Elispot results.Conclusions CD8 + T cells play an important role in the asymptomatic phase of HIV-1 infection,but with some interindividual variation.
Persistent Identifierhttp://hdl.handle.net/10722/164388
ISSN

 

DC FieldValueLanguage
dc.contributor.authorWang, Wen_US
dc.contributor.authorCong, Zen_US
dc.contributor.authorLiu, Hen_US
dc.contributor.authorTao, Zen_US
dc.contributor.authorLiu, Qen_US
dc.contributor.authorWei, Qen_US
dc.contributor.authorChen, Zen_US
dc.contributor.authorQin, Cen_US
dc.date.accessioned2012-09-20T07:58:49Z-
dc.date.available2012-09-20T07:58:49Z-
dc.date.issued2011en_US
dc.identifier.citation中國比較醫學雜誌, 2011, v. 21 n. 4, p. 1-5en_US
dc.identifier.citationChinese Journal of Comparative Medicine, 2011, v. 21 n. 4, p. 1-5-
dc.identifier.issn1671-7856-
dc.identifier.urihttp://hdl.handle.net/10722/164388-
dc.description.abstract目的 CD8+T細胞在一些病毒感染疾病的免疫反應中起著重要的作用,但CD8+T細胞在HIV無癥狀期的作用尚不明確,本研究通過體內CD8+T細胞剔除,研究CD8+T細胞對SHIV感染猴的影響,進一步了解艾滋病的發病機制。方法選擇8只SHIV病毒感染的恒河猴,均處于無癥狀期,隨機分成兩組,實驗組4只恒河猴在0、3、7 d注射抗CD8+T抗體cM-T807,不同的時間取外周血、腹股溝淋巴結。流式細胞術測定恒河猴外周血和淋巴結中CD8+T細胞數目,Real-time RT-PCR法測定實驗猴血漿病毒載量,并使用IFN-γElispot方法測定其對猴細胞免疫的影響。結果 CD8+T細胞敲除后,4只猴的病毒載量都轉陽,但反應性不一,HIV-1的靶細胞CD4+T細胞有輕微下降,后反彈,與病毒載量無相關性;CD8敲除猴的感染情況(血漿病毒載量和CD4細胞)比SHIV病毒急性感染輕,這與ELIPOT結果一致。結論 CD8+T細胞在HIV無癥狀期發揮重要的作用,但其作用具有個體差別。-
dc.description.abstractObjective CD8+ T cells play an important role in some viral diseases,but the role of CD8+ T cells is not yet fully clear in the asymptomatic phase of HIV infection.The aim of this study was to elucidate the impact of CD8 + T cell in SHIV-infected monkeys with in vivo CD8 + T cells depletion,and to further understand the pathogenesis of AIDS.Methods Eight SHIV-infected rhesus monkeys in the asymptomatic phase were selected and randomly divided into two groups.Anti-CD8 + T antibody cM-T807 were injected intravenously into four monkeys on days 0,3,and 7.Then the CD8 + T cell number in peripheral blood and lymph nodes in the monkeys were detected by flow cytometry,the plasma viral load by real-time RT-PCR,and the cellular immunity by IFN-γ Elispot assay.Results The viral load of the four monkeys was elevated to above of detected after the CD8 + T cell depletion,but the reactions were not uniform.CD4 + T cells,the HIV-1 target cells,rebound and dropped slightly.The infection of monkeys with CD8 + T cell depletion was lighter than that after the SHIV virus first infection(plasma viral load and CD4 cells),which was consistent with the Elispot results.Conclusions CD8 + T cells play an important role in the asymptomatic phase of HIV-1 infection,but with some interindividual variation.-
dc.languagechien_US
dc.publisher中國實驗動物學會-
dc.relation.ispartof中國比較醫學雜誌en_US
dc.relation.ispartofChinese Journal of Comparative Medicine-
dc.subjectMacaca mulatta (恒河猴)-
dc.subjectHIV-
dc.subjectT-Lymphocytes-
dc.subjectInfection-
dc.subjectViruses-
dc.titleEffect of CD8+ T Cell Depletion in Vivo on Monkeys with Chronic SHIV-KB9 Infectionen_US
dc.title體內CD8+T細胞剔除對無癥狀期SHIV感染猴的影-
dc.typeArticleen_US
dc.identifier.emailChen, Z: zchenai@hku.hken_US
dc.identifier.authorityChen, Z=rp00243en_US
dc.identifier.doi10.3969/j.issn.1671.7856.2011.04.001-
dc.identifier.hkuros206277en_US
dc.identifier.volume21en_US
dc.identifier.issue4en_US
dc.identifier.spage1en_US
dc.identifier.epage5en_US
dc.publisher.placeChina-

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