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Article: Role of PCDH10 and its hypermethylation in human gastric cancer

TitleRole of PCDH10 and its hypermethylation in human gastric cancer
Authors
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
Citation
Biochimica Et Biophysica Acta - Molecular Cell Research, 2012, v. 1823 n. 2, p. 298-305 How to Cite?
AbstractEpigenetic changes of genomic DNA are involved in the development and progression of many cancers. Aberrant methylation of CpG islands in the promoter regions of certain tumor-suppressor genes (TSG) is frequently observed in cancer cells. Protocadherin 10 (PCDH10), a member of the cadherin superfamily, is a recently identified putative TSG. PCDH10 is frequently silenced in many solid tumors. However, the role of PCDH10 in gastric cancer is largely unknown. In this study, we examined the expression and methylation status of PCDH10 in gastric cancer cells and tissues by real time PCR and methylation-specific PCR (MSP), and then investigated the biological function of PCDH10. We found that the expression of PCDH10 was markedly reduced in gastric cancer cells and tissues. The reduced expression correlated with hypermethylation of this gene in its promoter region, as demonstrated by MSP and bisulfite genomic sequencing (BGS) analysis. In addition, pharmacological demethylation using 5-Aza restored the expression of PCDH10 in gastric cancer cells. Over-expression of PCDH10 in gastric cancer cells suppressed cell proliferation and migration, but did not cause marked apoptosis. Over-expression of PCDH10 also suppressed growth of xenograft tumors in nude mice. Thus, PCDH10 functions as a TSG in gastric cancer, and might be a useful target for cancer therapy. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/164295
ISSN
2015 Impact Factor: 5.128
2015 SCImago Journal Rankings: 3.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Zen_HK
dc.contributor.authorChim, JCSen_HK
dc.contributor.authorYang, Men_HK
dc.contributor.authorYe, Jen_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorQiao, Len_HK
dc.date.accessioned2012-09-20T07:57:44Z-
dc.date.available2012-09-20T07:57:44Z-
dc.date.issued2012en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 2012, v. 1823 n. 2, p. 298-305en_HK
dc.identifier.issn0167-4889en_HK
dc.identifier.urihttp://hdl.handle.net/10722/164295-
dc.description.abstractEpigenetic changes of genomic DNA are involved in the development and progression of many cancers. Aberrant methylation of CpG islands in the promoter regions of certain tumor-suppressor genes (TSG) is frequently observed in cancer cells. Protocadherin 10 (PCDH10), a member of the cadherin superfamily, is a recently identified putative TSG. PCDH10 is frequently silenced in many solid tumors. However, the role of PCDH10 in gastric cancer is largely unknown. In this study, we examined the expression and methylation status of PCDH10 in gastric cancer cells and tissues by real time PCR and methylation-specific PCR (MSP), and then investigated the biological function of PCDH10. We found that the expression of PCDH10 was markedly reduced in gastric cancer cells and tissues. The reduced expression correlated with hypermethylation of this gene in its promoter region, as demonstrated by MSP and bisulfite genomic sequencing (BGS) analysis. In addition, pharmacological demethylation using 5-Aza restored the expression of PCDH10 in gastric cancer cells. Over-expression of PCDH10 in gastric cancer cells suppressed cell proliferation and migration, but did not cause marked apoptosis. Over-expression of PCDH10 also suppressed growth of xenograft tumors in nude mice. Thus, PCDH10 functions as a TSG in gastric cancer, and might be a useful target for cancer therapy. © 2011 Elsevier B.V.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcren_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Researchen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCadherins - genetics - metabolismen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshDNA Methylationen_HK
dc.subject.meshEpigenesis, Geneticen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGene Silencingen_HK
dc.subject.meshGenes, Tumor Suppressoren_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshNeoplasms, Experimental - genetics - metabolism - pathologyen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshStomach Neoplasms - genetics - metabolism - pathologyen_HK
dc.subject.meshTransplantation, Heterologousen_HK
dc.titleRole of PCDH10 and its hypermethylation in human gastric canceren_HK
dc.typeArticleen_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.emailQiao, L: lq8688@hotmail.comen_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.identifier.authorityQiao, L=rp00513en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbamcr.2011.11.011en_HK
dc.identifier.pmid22206871-
dc.identifier.scopuseid_2-s2.0-84862810286en_HK
dc.identifier.hkuros207234en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862810286&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1823en_HK
dc.identifier.issue2en_HK
dc.identifier.spage298en_HK
dc.identifier.epage305en_HK
dc.identifier.isiWOS:000301155700012-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, Z=37037717000en_HK
dc.identifier.scopusauthoridChim, JCS=55262215600en_HK
dc.identifier.scopusauthoridYang, M=7404927250en_HK
dc.identifier.scopusauthoridYe, J=23669624100en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridQiao, L=7202151719en_HK
dc.identifier.citeulike10158639-

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