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Article: Decellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studies

TitleDecellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studies
Authors
KeywordsDecellularization
Extracellular matrix
Intervertebral disc
Scaffold
Tissue engineering
Issue Date2013
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat
Citation
Acta Biomaterialia, 2013, v. 9 n. 2, p. 5262-5272 How to Cite?
AbstractLow back pain that is associated with disc degeneration contributes to a huge economic burden in the worldwide healthcare system. Traditional methods, such as spinal fusion, have been adopted to relieve mechanical back pain, but this is compromised by decreased spinal motion. Tissue engineering has attracted much attention, and aims to correct the changes fundamentally occurring in the discs by a combination of cell biology, molecular biology and engineering. Synthetic materials including poly(l-lactic acid) or poly(glycolic acid) and biomolecules like hyaluronic acid or collagen have been adopted in the development of disc scaffolds for studying therapeutic approaches. Nevertheless, the complex biological and mechanical environment of the intervertebral disc (IVD) makes the synthesis of an artificial IVD with biomaterials a difficult task. Thus the aim of this study was to develop a natural disc scaffold for culturing disc cells for future development of biological disc constructs. We adopted a combination of currently used decellularization techniques to decellularize bovine IVD to create a complete endplate-to-endplate IVD scaffold. By altering the chemical and physical decellularization parameters, we reported the removal of up to 70% of the endogenous cells, and were able to preserve the glycosaminoglycan content, collagen fibril architecture and mechanical properties of the discs. The reintroduction of nucleus pulposus cells into the scaffold indicated a high survival rate over 7 days, with cell penetration. We have shown here that conventional methods used for decellularizing thin tissues can also be applied to large organs, such as IVD. Our findings suggest the potential of using decellularized IVD as a scaffold for IVD bioengineering and culturing of cells in the context of the IVD niche. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/164260
ISSN
2015 Impact Factor: 6.008
2015 SCImago Journal Rankings: 2.020
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, LKYen_HK
dc.contributor.authorLeung, VYLen_HK
dc.contributor.authorTam, Ven_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorSze, KYen_HK
dc.contributor.authorCheung, KMCen_HK
dc.date.accessioned2012-09-20T07:56:55Z-
dc.date.available2012-09-20T07:56:55Z-
dc.date.issued2013en_HK
dc.identifier.citationActa Biomaterialia, 2013, v. 9 n. 2, p. 5262-5272en_HK
dc.identifier.issn1742-7061en_HK
dc.identifier.urihttp://hdl.handle.net/10722/164260-
dc.description.abstractLow back pain that is associated with disc degeneration contributes to a huge economic burden in the worldwide healthcare system. Traditional methods, such as spinal fusion, have been adopted to relieve mechanical back pain, but this is compromised by decreased spinal motion. Tissue engineering has attracted much attention, and aims to correct the changes fundamentally occurring in the discs by a combination of cell biology, molecular biology and engineering. Synthetic materials including poly(l-lactic acid) or poly(glycolic acid) and biomolecules like hyaluronic acid or collagen have been adopted in the development of disc scaffolds for studying therapeutic approaches. Nevertheless, the complex biological and mechanical environment of the intervertebral disc (IVD) makes the synthesis of an artificial IVD with biomaterials a difficult task. Thus the aim of this study was to develop a natural disc scaffold for culturing disc cells for future development of biological disc constructs. We adopted a combination of currently used decellularization techniques to decellularize bovine IVD to create a complete endplate-to-endplate IVD scaffold. By altering the chemical and physical decellularization parameters, we reported the removal of up to 70% of the endogenous cells, and were able to preserve the glycosaminoglycan content, collagen fibril architecture and mechanical properties of the discs. The reintroduction of nucleus pulposus cells into the scaffold indicated a high survival rate over 7 days, with cell penetration. We have shown here that conventional methods used for decellularizing thin tissues can also be applied to large organs, such as IVD. Our findings suggest the potential of using decellularized IVD as a scaffold for IVD bioengineering and culturing of cells in the context of the IVD niche. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomaten_HK
dc.relation.ispartofActa Biomaterialiaen_HK
dc.subjectDecellularizationen_HK
dc.subjectExtracellular matrixen_HK
dc.subjectIntervertebral discen_HK
dc.subjectScaffolden_HK
dc.subjectTissue engineeringen_HK
dc.titleDecellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studiesen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, VYL: vicleung@hku.hken_HK
dc.identifier.emailLu, WW: wwlu@hku.hken_HK
dc.identifier.emailSze, KY: kysze@hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.authorityLeung, VYL=rp01764en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.identifier.authoritySze, KY=rp00171en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.actbio.2012.09.005en_HK
dc.identifier.pmid23000521en_HK
dc.identifier.scopuseid_2-s2.0-84872075418en_HK
dc.identifier.hkuros210240en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84872075418&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue2en_HK
dc.identifier.spage5262en_HK
dc.identifier.epage5272en_HK
dc.identifier.isiWOS:000315170800034-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChan, LKY=35336076700en_HK
dc.identifier.scopusauthoridLeung, VYL=35337438900en_HK
dc.identifier.scopusauthoridTam, V=35977084900en_HK
dc.identifier.scopusauthoridLu, WW=55484358100en_HK
dc.identifier.scopusauthoridSze, KY=7006735060en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK

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