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Article: PHBV/PLLA-based composite scaffolds fabricated using an emulsion freezing/freeze-drying technique for bone tissue engineering: surface modification and in vitro biological evaluation

TitlePHBV/PLLA-based composite scaffolds fabricated using an emulsion freezing/freeze-drying technique for bone tissue engineering: surface modification and in vitro biological evaluation
Authors
Issue Date2012
PublisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/bf
Citation
Biofabrication, 2012, v. 4 n. 1, article no. 015003 How to Cite?
AbstractTissue engineering combines living cells with biodegradable materials and/or bioactive components. Composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramic with suitable properties are promising for bone tissue regeneration. In this paper, based on blending two biodegradable and biocompatible polymers, namely poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) with incorporated nano hydroxyapatite (HA), three-dimensional composite scaffolds with controlled microstructures and an interconnected porous structure, together with high porosity, were fabricated using an emulsion freezing/freeze-drying technique. The influence of various parameters involved in the emulsion freezing/freeze-drying technique was studied for the fabrication of good-quality polymer scaffolds based on PHBV polymers. The morphology, mechanical properties and crystallinity of PHBV/PLLA and HA in PHBV/PLLA composite scaffolds and PHBV polymer scaffolds were studied. The scaffolds were coated with collagen in order to improve wettability. During in vitro biological evaluation study, it was observed that SaOS-2 cells had high attachment on collagen-coated scaffolds. Significant improvement in cell proliferation and alkaline phosphatase activity for HA-incorporated composite scaffolds was observed due to the incorporation of HA. After 3 and 7 days of culture on all scaffolds, SaOS-2 cells also had normal morphology and growth. These results indicated that PHBV/PLLA-based scaffolds fabricated via an emulsion freezing/freeze-drying technique were favorable sites for osteoblastic cells and are promising for the applications of bone tissue engineering.
Persistent Identifierhttp://hdl.handle.net/10722/164211
ISSN
2015 Impact Factor: 4.702
2015 SCImago Journal Rankings: 1.505
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSultana, Nen_US
dc.contributor.authorWang, Men_US
dc.date.accessioned2012-09-20T07:56:39Z-
dc.date.available2012-09-20T07:56:39Z-
dc.date.issued2012en_US
dc.identifier.citationBiofabrication, 2012, v. 4 n. 1, article no. 015003en_US
dc.identifier.issn1758-5082-
dc.identifier.urihttp://hdl.handle.net/10722/164211-
dc.description.abstractTissue engineering combines living cells with biodegradable materials and/or bioactive components. Composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramic with suitable properties are promising for bone tissue regeneration. In this paper, based on blending two biodegradable and biocompatible polymers, namely poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) with incorporated nano hydroxyapatite (HA), three-dimensional composite scaffolds with controlled microstructures and an interconnected porous structure, together with high porosity, were fabricated using an emulsion freezing/freeze-drying technique. The influence of various parameters involved in the emulsion freezing/freeze-drying technique was studied for the fabrication of good-quality polymer scaffolds based on PHBV polymers. The morphology, mechanical properties and crystallinity of PHBV/PLLA and HA in PHBV/PLLA composite scaffolds and PHBV polymer scaffolds were studied. The scaffolds were coated with collagen in order to improve wettability. During in vitro biological evaluation study, it was observed that SaOS-2 cells had high attachment on collagen-coated scaffolds. Significant improvement in cell proliferation and alkaline phosphatase activity for HA-incorporated composite scaffolds was observed due to the incorporation of HA. After 3 and 7 days of culture on all scaffolds, SaOS-2 cells also had normal morphology and growth. These results indicated that PHBV/PLLA-based scaffolds fabricated via an emulsion freezing/freeze-drying technique were favorable sites for osteoblastic cells and are promising for the applications of bone tissue engineering.-
dc.languageengen_US
dc.publisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/bfen_US
dc.relation.ispartofBiofabricationen_US
dc.rightsBiofabrication. Copyright © Institute of Physics Publishing Ltd..en_US
dc.subject.meshBone Substitutes - chemistry - pharmacology-
dc.subject.meshDurapatite - chemistry - pharmacology-
dc.subject.meshOsteoblasts - cytology - drug effects - metabolism-
dc.subject.meshPolyesters - chemistry - pharmacology-
dc.subject.meshTissue Engineering - instrumentation-
dc.titlePHBV/PLLA-based composite scaffolds fabricated using an emulsion freezing/freeze-drying technique for bone tissue engineering: surface modification and in vitro biological evaluationen_US
dc.typeArticleen_US
dc.identifier.emailSultana, N: naznin@biomedical.utm.myen_US
dc.identifier.emailWang, M: memwang@hku.hk-
dc.identifier.authorityWang, M=rp00185en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1088/1758-5082/4/1/015003-
dc.identifier.pmid22258057-
dc.identifier.scopuseid_2-s2.0-84857864802-
dc.identifier.hkuros207501en_US
dc.identifier.volume4en_US
dc.identifier.issue1, article no. 015003-
dc.identifier.isiWOS:000301863800005-
dc.publisher.placeUnited Kingdom-

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