File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Electrospun poly(Hydroxybutyrate-co-Hydroxyvalerate) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers: Characterization and biological evaluation

TitleElectrospun poly(Hydroxybutyrate-co-Hydroxyvalerate) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers: Characterization and biological evaluation
Authors
KeywordsCell culture
Cross-aligned fibers
Electrospinning
Parallel-aligned fibers
Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)
Issue Date2011
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
Citation
Journal of Biomaterials Science Polymer Edition, 2011, v. 22 n. 18, p. 2475-2497 How to Cite?
AbstractPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers were fabricated through electrospinning with the help of a rotating cylinder as fiber collector and auxiliary electrodes, and their application as tissue-engineering scaffolds was assessed. First, these membranes were characterized in terms of fiber diameter, spacing between adjacent aligned fibers or interstitial pore diameter, degree of fiber alignment, wettability and tensile properties. Then, human osteoblast-like cells (SaOS-2) were seeded and cultured on these membranes for up to 14 days. The cell morphology and proliferation were evaluated at different cell culture times. Membranes consisting of random fibers or parallel-aligned fibers were obtained when the rotational speed of the cylinder was 500 rpm or 3000 rpm, respectively. A very high rotational speed of 15 000 rpm resulted in the formation of parallel-aligned fibers having low or no spacing between the aligned fibers. Membranes consisting of cross-aligned fibers were made at the rotational speed of 3000 rpm and micrometer-sized fiber spacing was observed in these membranes. The alignment of fibers led to enhanced wettability of fibrous membranes. Tensile testing revealed that the parallel-aligned fibrous membranes were strong in the longitudinal direction but weak in the transverse direction. The cross-aligned fibrous membranes did not exhibit particularly weak tensile properties in any direction. In vitro biological evaluation showed that SaOS-2 cells spread randomly on membranes of random fibers but elongated in membranes of aligned fibers. All membranes supported cell proliferation in spite of the differences in cell morphology. © 2011 Koninklijke Brill NV, Leiden.
Persistent Identifierhttp://hdl.handle.net/10722/164204
ISSN
2014 Impact Factor: 1.648
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTong, HWen_US
dc.contributor.authorWang, Men_US
dc.contributor.authorLu, WWen_US
dc.date.accessioned2012-09-20T07:56:36Z-
dc.date.available2012-09-20T07:56:36Z-
dc.date.issued2011en_US
dc.identifier.citationJournal of Biomaterials Science Polymer Edition, 2011, v. 22 n. 18, p. 2475-2497en_US
dc.identifier.issn0920-5063-
dc.identifier.urihttp://hdl.handle.net/10722/164204-
dc.description.abstractPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers were fabricated through electrospinning with the help of a rotating cylinder as fiber collector and auxiliary electrodes, and their application as tissue-engineering scaffolds was assessed. First, these membranes were characterized in terms of fiber diameter, spacing between adjacent aligned fibers or interstitial pore diameter, degree of fiber alignment, wettability and tensile properties. Then, human osteoblast-like cells (SaOS-2) were seeded and cultured on these membranes for up to 14 days. The cell morphology and proliferation were evaluated at different cell culture times. Membranes consisting of random fibers or parallel-aligned fibers were obtained when the rotational speed of the cylinder was 500 rpm or 3000 rpm, respectively. A very high rotational speed of 15 000 rpm resulted in the formation of parallel-aligned fibers having low or no spacing between the aligned fibers. Membranes consisting of cross-aligned fibers were made at the rotational speed of 3000 rpm and micrometer-sized fiber spacing was observed in these membranes. The alignment of fibers led to enhanced wettability of fibrous membranes. Tensile testing revealed that the parallel-aligned fibrous membranes were strong in the longitudinal direction but weak in the transverse direction. The cross-aligned fibrous membranes did not exhibit particularly weak tensile properties in any direction. In vitro biological evaluation showed that SaOS-2 cells spread randomly on membranes of random fibers but elongated in membranes of aligned fibers. All membranes supported cell proliferation in spite of the differences in cell morphology. © 2011 Koninklijke Brill NV, Leiden.-
dc.languageengen_US
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/currenten_US
dc.relation.ispartofJournal of Biomaterials Science Polymer Editionen_US
dc.subjectCell culture-
dc.subjectCross-aligned fibers-
dc.subjectElectrospinning-
dc.subjectParallel-aligned fibers-
dc.subjectPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)-
dc.titleElectrospun poly(Hydroxybutyrate-co-Hydroxyvalerate) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers: Characterization and biological evaluationen_US
dc.typeArticleen_US
dc.identifier.emailTong, HW: meboris@hku.hken_US
dc.identifier.emailWang, M: memwang@hku.hken_US
dc.identifier.emailLu, WW: wwlu@hku.hken_US
dc.identifier.authorityWang, M=rp00185en_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1163/092050610X540675-
dc.identifier.scopuseid_2-s2.0-80755188056-
dc.identifier.hkuros207448en_US
dc.identifier.volume22en_US
dc.identifier.issue18-
dc.identifier.spage2475en_US
dc.identifier.epage2497en_US
dc.identifier.isiWOS:000297423800007-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats