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Article: Electrospun poly(Hydroxybutyrate-co-Hydroxyvalerate) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers: Characterization and biological evaluation
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TitleElectrospun poly(Hydroxybutyrate-co-Hydroxyvalerate) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers: Characterization and biological evaluation
 
AuthorsTong, HW1
Wang, M1
Lu, WW1
 
KeywordsCell culture
Cross-aligned fibers
Electrospinning
Parallel-aligned fibers
Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)
 
Issue Date2011
 
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
 
CitationJournal of Biomaterials Science Polymer Edition, 2011, v. 22 n. 18, p. 2475-2497 [How to Cite?]
DOI: http://dx.doi.org/10.1163/092050610X540675
 
AbstractPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers were fabricated through electrospinning with the help of a rotating cylinder as fiber collector and auxiliary electrodes, and their application as tissue-engineering scaffolds was assessed. First, these membranes were characterized in terms of fiber diameter, spacing between adjacent aligned fibers or interstitial pore diameter, degree of fiber alignment, wettability and tensile properties. Then, human osteoblast-like cells (SaOS-2) were seeded and cultured on these membranes for up to 14 days. The cell morphology and proliferation were evaluated at different cell culture times. Membranes consisting of random fibers or parallel-aligned fibers were obtained when the rotational speed of the cylinder was 500 rpm or 3000 rpm, respectively. A very high rotational speed of 15 000 rpm resulted in the formation of parallel-aligned fibers having low or no spacing between the aligned fibers. Membranes consisting of cross-aligned fibers were made at the rotational speed of 3000 rpm and micrometer-sized fiber spacing was observed in these membranes. The alignment of fibers led to enhanced wettability of fibrous membranes. Tensile testing revealed that the parallel-aligned fibrous membranes were strong in the longitudinal direction but weak in the transverse direction. The cross-aligned fibrous membranes did not exhibit particularly weak tensile properties in any direction. In vitro biological evaluation showed that SaOS-2 cells spread randomly on membranes of random fibers but elongated in membranes of aligned fibers. All membranes supported cell proliferation in spite of the differences in cell morphology. © 2011 Koninklijke Brill NV, Leiden.
 
ISSN0920-5063
2013 Impact Factor: 1.357
 
DOIhttp://dx.doi.org/10.1163/092050610X540675
 
ISI Accession Number IDWOS:000297423800007
 
DC FieldValue
dc.contributor.authorTong, HW
 
dc.contributor.authorWang, M
 
dc.contributor.authorLu, WW
 
dc.date.accessioned2012-09-20T07:56:36Z
 
dc.date.available2012-09-20T07:56:36Z
 
dc.date.issued2011
 
dc.description.abstractPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers were fabricated through electrospinning with the help of a rotating cylinder as fiber collector and auxiliary electrodes, and their application as tissue-engineering scaffolds was assessed. First, these membranes were characterized in terms of fiber diameter, spacing between adjacent aligned fibers or interstitial pore diameter, degree of fiber alignment, wettability and tensile properties. Then, human osteoblast-like cells (SaOS-2) were seeded and cultured on these membranes for up to 14 days. The cell morphology and proliferation were evaluated at different cell culture times. Membranes consisting of random fibers or parallel-aligned fibers were obtained when the rotational speed of the cylinder was 500 rpm or 3000 rpm, respectively. A very high rotational speed of 15 000 rpm resulted in the formation of parallel-aligned fibers having low or no spacing between the aligned fibers. Membranes consisting of cross-aligned fibers were made at the rotational speed of 3000 rpm and micrometer-sized fiber spacing was observed in these membranes. The alignment of fibers led to enhanced wettability of fibrous membranes. Tensile testing revealed that the parallel-aligned fibrous membranes were strong in the longitudinal direction but weak in the transverse direction. The cross-aligned fibrous membranes did not exhibit particularly weak tensile properties in any direction. In vitro biological evaluation showed that SaOS-2 cells spread randomly on membranes of random fibers but elongated in membranes of aligned fibers. All membranes supported cell proliferation in spite of the differences in cell morphology. © 2011 Koninklijke Brill NV, Leiden.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal of Biomaterials Science Polymer Edition, 2011, v. 22 n. 18, p. 2475-2497 [How to Cite?]
DOI: http://dx.doi.org/10.1163/092050610X540675
 
dc.identifier.doihttp://dx.doi.org/10.1163/092050610X540675
 
dc.identifier.epage2497
 
dc.identifier.hkuros207448
 
dc.identifier.isiWOS:000297423800007
 
dc.identifier.issn0920-5063
2013 Impact Factor: 1.357
 
dc.identifier.issue18
 
dc.identifier.scopuseid_2-s2.0-80755188056
 
dc.identifier.spage2475
 
dc.identifier.urihttp://hdl.handle.net/10722/164204
 
dc.identifier.volume22
 
dc.languageeng
 
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Biomaterials Science Polymer Edition
 
dc.subjectCell culture
 
dc.subjectCross-aligned fibers
 
dc.subjectElectrospinning
 
dc.subjectParallel-aligned fibers
 
dc.subjectPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)
 
dc.titleElectrospun poly(Hydroxybutyrate-co-Hydroxyvalerate) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers: Characterization and biological evaluation
 
dc.typeArticle
 
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<item><contributor.author>Tong, HW</contributor.author>
<contributor.author>Wang, M</contributor.author>
<contributor.author>Lu, WW</contributor.author>
<date.accessioned>2012-09-20T07:56:36Z</date.accessioned>
<date.available>2012-09-20T07:56:36Z</date.available>
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<description.abstract>Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) fibrous membranes consisting of parallel-aligned fibers or cross-aligned fibers were fabricated through electrospinning with the help of a rotating cylinder as fiber collector and auxiliary electrodes, and their application as tissue-engineering scaffolds was assessed. First, these membranes were characterized in terms of fiber diameter, spacing between adjacent aligned fibers or interstitial pore diameter, degree of fiber alignment, wettability and tensile properties. Then, human osteoblast-like cells (SaOS-2) were seeded and cultured on these membranes for up to 14 days. The cell morphology and proliferation were evaluated at different cell culture times. Membranes consisting of random fibers or parallel-aligned fibers were obtained when the rotational speed of the cylinder was 500 rpm or 3000 rpm, respectively. A very high rotational speed of 15 000 rpm resulted in the formation of parallel-aligned fibers having low or no spacing between the aligned fibers. Membranes consisting of cross-aligned fibers were made at the rotational speed of 3000 rpm and micrometer-sized fiber spacing was observed in these membranes. The alignment of fibers led to enhanced wettability of fibrous membranes. Tensile testing revealed that the parallel-aligned fibrous membranes were strong in the longitudinal direction but weak in the transverse direction. The cross-aligned fibrous membranes did not exhibit particularly weak tensile properties in any direction. In vitro biological evaluation showed that SaOS-2 cells spread randomly on membranes of random fibers but elongated in membranes of aligned fibers. All membranes supported cell proliferation in spite of the differences in cell morphology. &#169; 2011 Koninklijke Brill NV, Leiden.</description.abstract>
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<subject>Cell culture</subject>
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<subject>Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)</subject>
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Author Affiliations
  1. The University of Hong Kong