File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1107/S0907444903007054
- Scopus: eid_2-s2.0-0038455892
- PMID: 12832769
- WOS: WOS:000183809900017
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2
Title | Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2 |
---|---|
Authors | |
Issue Date | 2003 |
Publisher | International Union of Crystallography. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1 |
Citation | Acta Crystallographica Section D: Biological Crystallography, 2003, v. 59 n. 7, p. 1230-1233 How to Cite? |
Abstract | The BRCT domain from Rhp9 (a Schizosaccharomyces pombe DNA-damage checkpoint protein) has been expressed, purified and crystallized. Overexpression in bacterial cells was achieved by minimizing aeration during host cell growth. A robotic screen was used to determine the solubility parameters; concentration of the protein was achieved by exploiting this information. Single crystals suitable for X-ray analysis were obtained in two forms by vapour diffusion (trigonal, unit-cell parameters a = b = 228.04, c = 70.42 A, and tetragonal, P4/m Laue group symmetry, unit-cell parameters a = b = 72.3, c = 91.1 A). |
Persistent Identifier | http://hdl.handle.net/10722/163627 |
ISSN | 2013 Impact Factor: 7.232 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hinks, JA | - |
dc.contributor.author | Roe, M | - |
dc.contributor.author | Ho, JCY | - |
dc.contributor.author | Watts, FZ | - |
dc.contributor.author | Phelan, J | - |
dc.contributor.author | McAllister, M | - |
dc.contributor.author | Pearl, LH | - |
dc.date.accessioned | 2012-09-14T03:32:31Z | - |
dc.date.available | 2012-09-14T03:32:31Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Acta Crystallographica Section D: Biological Crystallography, 2003, v. 59 n. 7, p. 1230-1233 | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | http://hdl.handle.net/10722/163627 | - |
dc.description.abstract | The BRCT domain from Rhp9 (a Schizosaccharomyces pombe DNA-damage checkpoint protein) has been expressed, purified and crystallized. Overexpression in bacterial cells was achieved by minimizing aeration during host cell growth. A robotic screen was used to determine the solubility parameters; concentration of the protein was achieved by exploiting this information. Single crystals suitable for X-ray analysis were obtained in two forms by vapour diffusion (trigonal, unit-cell parameters a = b = 228.04, c = 70.42 A, and tetragonal, P4/m Laue group symmetry, unit-cell parameters a = b = 72.3, c = 91.1 A). | - |
dc.language | eng | - |
dc.publisher | International Union of Crystallography. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1 | - |
dc.relation.ispartof | Acta Crystallographica Section D: Biological Crystallography | - |
dc.subject.mesh | Cell Cycle Proteins - chemistry | - |
dc.subject.mesh | Cloning, Molecular - methods | - |
dc.subject.mesh | Crystallization - methods | - |
dc.subject.mesh | Nuclear Proteins - chemistry | - |
dc.subject.mesh | Schizosaccharomyces pombe Proteins - chemistry | - |
dc.title | Expression, purification and preliminary X-ray analysis of the BRCT domain from Rhp9/Crb2 | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ho, JCY: jennyho@hku.hk | - |
dc.identifier.email | Pearl, LH: l.pearl@icr.ac.uk | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1107/S0907444903007054 | - |
dc.identifier.pmid | 12832769 | - |
dc.identifier.scopus | eid_2-s2.0-0038455892 | - |
dc.identifier.hkuros | 82771 | - |
dc.identifier.volume | 59 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | 1230 | - |
dc.identifier.epage | 1233 | - |
dc.identifier.isi | WOS:000183809900017 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0907-4449 | - |