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Article: Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol
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TitleDisruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol
 
AuthorsMa, Y6 1
Han, J6
Guo, Y6
Lam, PKS7
Wu, RSS5
Giesy, JP5 2 7 4
Zhang, X3
Zhou, B6
 
KeywordsH295R
Hormones
HPG axis
In vitro
In vivo
Zebrafish
 
Issue Date2012
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
CitationAquatic Toxicology, 2012, v. 106-107, p. 173-181 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2011.11.006
 
AbstractChlorophenols in the aquatic environment have been of concern due to their potential effects on human and wildlife. In the present study, the endocrine disrupting effects of 2,4-dichlorophenol (2,4-DCP) were investigated in vitro and in vivo. In the in vitro assay, H295R human adrenocortical carcinoma cells were used to determine the potential effects of 2,4-DCP on steroidogenesis. Exposure to 0, 0.1, 0.3 or 1.0. mg 2,4-DCP/L resulted in less production of 17β-estradiol (E2) and alterations in transcript expressions of genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3βHSD, 17βHSD and StAR. In the in vivo study, effects of 0, 0.03, 0.1 or 0.3. mg 2,4-DCP/L on concentrations of steroid hormones in plasma of adult zebrafish (Danio rerio) were measured and expression of mRNA of selected genes in hypothalamic-pituitary-gonadal (HPG) axis and liver were determined. Exposure of zebrafish to 2,4-DCP resulted in lesser concentrations of E2 accompanied by down-regulation of CYP19A mRNA in the females. In males, exposure to 2,4-DCP resulted in greater concentrations of testosterone (T) and E2 along with greater mRNA expression of CYP17 and CYP19A. The mRNA expression of prostaglandin synthase (Ptgs2) gene, which regulates ovulation, was down-regulated in females, but up-regulated in males. The hepatic estrogenic receptor (ERα and ERβ) and vitellogenin (VTG1 and VTG3) mRNAs were up-regulated in both females and males. The average number of eggs spawned was significantly less upon exposure to 2,4-DCP. Exposure of adult zebrafish to 2,4-DCP resulted in lesser rates of hatching of eggs. The results demonstrated that 2,4-DCP modulates transcription of steroidogenetic genes in both H295R cells and in the zebrafish HPG-axis and disrupts steroidogenesis, which in turn, can cause adverse effects on reproduction in fish. © 2011 Elsevier B.V.
 
ISSN0166-445X
2013 Impact Factor: 3.513
 
DOIhttp://dx.doi.org/10.1016/j.aquatox.2011.11.006
 
ISI Accession Number IDWOS:000300205300021
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorMa, Y
 
dc.contributor.authorHan, J
 
dc.contributor.authorGuo, Y
 
dc.contributor.authorLam, PKS
 
dc.contributor.authorWu, RSS
 
dc.contributor.authorGiesy, JP
 
dc.contributor.authorZhang, X
 
dc.contributor.authorZhou, B
 
dc.date.accessioned2012-09-07T02:20:55Z
 
dc.date.available2012-09-07T02:20:55Z
 
dc.date.issued2012
 
dc.description.abstractChlorophenols in the aquatic environment have been of concern due to their potential effects on human and wildlife. In the present study, the endocrine disrupting effects of 2,4-dichlorophenol (2,4-DCP) were investigated in vitro and in vivo. In the in vitro assay, H295R human adrenocortical carcinoma cells were used to determine the potential effects of 2,4-DCP on steroidogenesis. Exposure to 0, 0.1, 0.3 or 1.0. mg 2,4-DCP/L resulted in less production of 17β-estradiol (E2) and alterations in transcript expressions of genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3βHSD, 17βHSD and StAR. In the in vivo study, effects of 0, 0.03, 0.1 or 0.3. mg 2,4-DCP/L on concentrations of steroid hormones in plasma of adult zebrafish (Danio rerio) were measured and expression of mRNA of selected genes in hypothalamic-pituitary-gonadal (HPG) axis and liver were determined. Exposure of zebrafish to 2,4-DCP resulted in lesser concentrations of E2 accompanied by down-regulation of CYP19A mRNA in the females. In males, exposure to 2,4-DCP resulted in greater concentrations of testosterone (T) and E2 along with greater mRNA expression of CYP17 and CYP19A. The mRNA expression of prostaglandin synthase (Ptgs2) gene, which regulates ovulation, was down-regulated in females, but up-regulated in males. The hepatic estrogenic receptor (ERα and ERβ) and vitellogenin (VTG1 and VTG3) mRNAs were up-regulated in both females and males. The average number of eggs spawned was significantly less upon exposure to 2,4-DCP. Exposure of adult zebrafish to 2,4-DCP resulted in lesser rates of hatching of eggs. The results demonstrated that 2,4-DCP modulates transcription of steroidogenetic genes in both H295R cells and in the zebrafish HPG-axis and disrupts steroidogenesis, which in turn, can cause adverse effects on reproduction in fish. © 2011 Elsevier B.V.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationAquatic Toxicology, 2012, v. 106-107, p. 173-181 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.aquatox.2011.11.006
 
dc.identifier.citeulike10085914
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquatox.2011.11.006
 
dc.identifier.epage181
 
dc.identifier.hkuros208828
 
dc.identifier.isiWOS:000300205300021
 
dc.identifier.issn0166-445X
2013 Impact Factor: 3.513
 
dc.identifier.pmid22155427
 
dc.identifier.scopuseid_2-s2.0-82955189216
 
dc.identifier.spage173
 
dc.identifier.urihttp://hdl.handle.net/10722/163580
 
dc.identifier.volume106-107
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofAquatic Toxicology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnthelmintics - toxicity
 
dc.subject.meshChlorophenols - toxicity
 
dc.subject.meshEndocrine Disruptors - toxicity
 
dc.subject.meshEndocrine System - drug effects - metabolism
 
dc.subject.meshWater Pollutants, Chemical - toxicity
 
dc.subjectH295R
 
dc.subjectHormones
 
dc.subjectHPG axis
 
dc.subjectIn vitro
 
dc.subjectIn vivo
 
dc.subjectZebrafish
 
dc.titleDisruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol
 
dc.typeArticle
 
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<contributor.author>Wu, RSS</contributor.author>
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Author Affiliations
  1. Graduate University of Chinese Academy of Sciences
  2. University of Saskatchewan
  3. Nanjing University
  4. King Saud University College of Science
  5. The University of Hong Kong
  6. Institute of Hydrobiology, Chinese Academy of Sciences
  7. City University of Hong Kong