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Article: Disruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol

TitleDisruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenol
Authors
KeywordsH295R
Hormones
HPG axis
In vitro
In vivo
Zebrafish
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox
Citation
Aquatic Toxicology, 2012, v. 106-107, p. 173-181 How to Cite?
Abstract
Chlorophenols in the aquatic environment have been of concern due to their potential effects on human and wildlife. In the present study, the endocrine disrupting effects of 2,4-dichlorophenol (2,4-DCP) were investigated in vitro and in vivo. In the in vitro assay, H295R human adrenocortical carcinoma cells were used to determine the potential effects of 2,4-DCP on steroidogenesis. Exposure to 0, 0.1, 0.3 or 1.0. mg 2,4-DCP/L resulted in less production of 17β-estradiol (E2) and alterations in transcript expressions of genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3βHSD, 17βHSD and StAR. In the in vivo study, effects of 0, 0.03, 0.1 or 0.3. mg 2,4-DCP/L on concentrations of steroid hormones in plasma of adult zebrafish (Danio rerio) were measured and expression of mRNA of selected genes in hypothalamic-pituitary-gonadal (HPG) axis and liver were determined. Exposure of zebrafish to 2,4-DCP resulted in lesser concentrations of E2 accompanied by down-regulation of CYP19A mRNA in the females. In males, exposure to 2,4-DCP resulted in greater concentrations of testosterone (T) and E2 along with greater mRNA expression of CYP17 and CYP19A. The mRNA expression of prostaglandin synthase (Ptgs2) gene, which regulates ovulation, was down-regulated in females, but up-regulated in males. The hepatic estrogenic receptor (ERα and ERβ) and vitellogenin (VTG1 and VTG3) mRNAs were up-regulated in both females and males. The average number of eggs spawned was significantly less upon exposure to 2,4-DCP. Exposure of adult zebrafish to 2,4-DCP resulted in lesser rates of hatching of eggs. The results demonstrated that 2,4-DCP modulates transcription of steroidogenetic genes in both H295R cells and in the zebrafish HPG-axis and disrupts steroidogenesis, which in turn, can cause adverse effects on reproduction in fish. © 2011 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/163580
ISSN
2013 Impact Factor: 3.513
ISI Accession Number ID
References

 

Author Affiliations
  1. Graduate University of Chinese Academy of Sciences
  2. University of Saskatchewan
  3. Nanjing University
  4. King Saud University College of Science
  5. The University of Hong Kong
  6. Institute of Hydrobiology, Chinese Academy of Sciences
  7. City University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorMa, Yen_HK
dc.contributor.authorHan, Jen_HK
dc.contributor.authorGuo, Yen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorGiesy, JPen_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorZhou, Ben_HK
dc.date.accessioned2012-09-07T02:20:55Z-
dc.date.available2012-09-07T02:20:55Z-
dc.date.issued2012en_HK
dc.identifier.citationAquatic Toxicology, 2012, v. 106-107, p. 173-181en_HK
dc.identifier.issn0166-445Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/163580-
dc.description.abstractChlorophenols in the aquatic environment have been of concern due to their potential effects on human and wildlife. In the present study, the endocrine disrupting effects of 2,4-dichlorophenol (2,4-DCP) were investigated in vitro and in vivo. In the in vitro assay, H295R human adrenocortical carcinoma cells were used to determine the potential effects of 2,4-DCP on steroidogenesis. Exposure to 0, 0.1, 0.3 or 1.0. mg 2,4-DCP/L resulted in less production of 17β-estradiol (E2) and alterations in transcript expressions of genes involved in steroidogenesis, including cytochrome P450 (CYP11A, CYP17, CYP19), 3βHSD, 17βHSD and StAR. In the in vivo study, effects of 0, 0.03, 0.1 or 0.3. mg 2,4-DCP/L on concentrations of steroid hormones in plasma of adult zebrafish (Danio rerio) were measured and expression of mRNA of selected genes in hypothalamic-pituitary-gonadal (HPG) axis and liver were determined. Exposure of zebrafish to 2,4-DCP resulted in lesser concentrations of E2 accompanied by down-regulation of CYP19A mRNA in the females. In males, exposure to 2,4-DCP resulted in greater concentrations of testosterone (T) and E2 along with greater mRNA expression of CYP17 and CYP19A. The mRNA expression of prostaglandin synthase (Ptgs2) gene, which regulates ovulation, was down-regulated in females, but up-regulated in males. The hepatic estrogenic receptor (ERα and ERβ) and vitellogenin (VTG1 and VTG3) mRNAs were up-regulated in both females and males. The average number of eggs spawned was significantly less upon exposure to 2,4-DCP. Exposure of adult zebrafish to 2,4-DCP resulted in lesser rates of hatching of eggs. The results demonstrated that 2,4-DCP modulates transcription of steroidogenetic genes in both H295R cells and in the zebrafish HPG-axis and disrupts steroidogenesis, which in turn, can cause adverse effects on reproduction in fish. © 2011 Elsevier B.V.en_HK
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatoxen_HK
dc.relation.ispartofAquatic Toxicologyen_HK
dc.subjectH295Ren_HK
dc.subjectHormonesen_HK
dc.subjectHPG axisen_HK
dc.subjectIn vitroen_HK
dc.subjectIn vivoen_HK
dc.subjectZebrafishen_HK
dc.subject.meshAnthelmintics - toxicity-
dc.subject.meshChlorophenols - toxicity-
dc.subject.meshEndocrine Disruptors - toxicity-
dc.subject.meshEndocrine System - drug effects - metabolism-
dc.subject.meshWater Pollutants, Chemical - toxicity-
dc.titleDisruption of endocrine function in in vitro H295R cell-based and in in vivo assay in zebrafish by 2,4-dichlorophenolen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aquatox.2011.11.006en_HK
dc.identifier.pmid22155427en_HK
dc.identifier.scopuseid_2-s2.0-82955189216en_HK
dc.identifier.hkuros208828-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-82955189216&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume106-107en_HK
dc.identifier.spage173en_HK
dc.identifier.epage181en_HK
dc.identifier.isiWOS:000300205300021-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridMa, Y=27867767600en_HK
dc.identifier.scopusauthoridHan, J=34770651700en_HK
dc.identifier.scopusauthoridGuo, Y=35798216000en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridGiesy, JP=35459135300en_HK
dc.identifier.scopusauthoridZhang, X=36086912900en_HK
dc.identifier.scopusauthoridZhou, B=7401906781en_HK
dc.identifier.citeulike10085914-

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