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Article: Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro

TitleAngiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro
Authors
Issue Date2012
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
Citation
Journal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 447-453 How to Cite?
AbstractEthnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration. © 2011 Elsevier Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/163447
ISSN
2014 Impact Factor: 2.998
2014 SCImago Journal Rankings: 1.069
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, HYGen_US
dc.contributor.authorHui, MNYen_US
dc.contributor.authorLi, Len_US
dc.contributor.authorLee, SMYen_US
dc.contributor.authorLeung, AYHen_US
dc.contributor.authorCheng, SHen_US
dc.date.accessioned2012-09-05T05:31:26Z-
dc.date.available2012-09-05T05:31:26Z-
dc.date.issued2012en_US
dc.identifier.citationJournal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 447-453en_US
dc.identifier.issn0378-8741en_US
dc.identifier.urihttp://hdl.handle.net/10722/163447-
dc.description.abstractEthnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration. © 2011 Elsevier Ireland Ltd.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharmen_US
dc.relation.ispartofJournal of Ethnopharmacologyen_US
dc.subject.meshAngiogenesis Inducing Agents - Pharmacology - Therapeutic Use - Toxicityen_US
dc.subject.meshAngiogenic Proteins - Geneticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Genetically Modifieden_US
dc.subject.meshAorta - Drug Effectsen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshDiabetic Foot - Drug Therapyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDrugs, Chinese Herbal - Pharmacology - Therapeutic Use - Toxicityen_US
dc.subject.meshEmbryo, Nonmammalian - Blood Supply - Drug Effectsen_US
dc.subject.meshEndothelial Cells - Drug Effects - Metabolismen_US
dc.subject.meshG2 Phase Cell Cycle Checkpoints - Drug Effectsen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshLethal Dose 50en_US
dc.subject.meshMap Kinase Signaling System - Drug Effects - Geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshNeovascularization, Physiologic - Drug Effects - Geneticsen_US
dc.subject.meshProto-Oncogene Protein C-Fli-1 - Geneticsen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReal-Time Polymerase Chain Reactionen_US
dc.subject.meshS Phase Cell Cycle Checkpoints - Drug Effectsen_US
dc.subject.meshZebrafish - Embryology - Geneticsen_US
dc.titleAngiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitroen_US
dc.typeArticleen_US
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_US
dc.identifier.authorityLeung, AYH=rp00265en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jep.2011.11.031en_US
dc.identifier.pmid22138660en_US
dc.identifier.scopuseid_2-s2.0-84856006882en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84856006882&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume139en_US
dc.identifier.issue2en_US
dc.identifier.spage447en_US
dc.identifier.epage453en_US
dc.identifier.isiWOS:000302505900018-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridTse, HYG=36344486800en_US
dc.identifier.scopusauthoridHui, MNY=17342146300en_US
dc.identifier.scopusauthoridLi, L=36067133800en_US
dc.identifier.scopusauthoridLee, SMY=35233892600en_US
dc.identifier.scopusauthoridLeung, AYH=7403012668en_US
dc.identifier.scopusauthoridCheng, SH=7404684691en_US
dc.identifier.citeulike10097018-

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