Article: Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro

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TitleAngiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro
AuthorsTse, HYG2
Hui, MNY2
Li, L2
Lee, SMY3
Leung, AYH1
Cheng, SH2
Issue Date2012
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
CitationJournal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 447-453 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jep.2011.11.031
AbstractEthnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration. © 2011 Elsevier Ireland Ltd.
ISSN0378-8741
2011 Impact Factor: 3.014
2011 SCImago Journal Rankings: 0.114
DOIhttp://dx.doi.org/10.1016/j.jep.2011.11.031
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTse, HYG
dc.contributor.authorHui, MNY
dc.contributor.authorLi, L
dc.contributor.authorLee, SMY
dc.contributor.authorLeung, AYH
dc.contributor.authorCheng, SH
dc.date.accessioned2012-09-05T05:31:26Z
dc.date.available2012-09-05T05:31:26Z
dc.date.issued2012
dc.description.abstractEthnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration. © 2011 Elsevier Ireland Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 447-453 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jep.2011.11.031
dc.identifier.citeulike10097018
dc.identifier.doihttp://dx.doi.org/10.1016/j.jep.2011.11.031
dc.identifier.epage453
dc.identifier.issn0378-8741
2011 Impact Factor: 3.014
2011 SCImago Journal Rankings: 0.114
dc.identifier.issue2
dc.identifier.pmid22138660
dc.identifier.scopuseid_2-s2.0-84856006882
dc.identifier.spage447
dc.identifier.urihttp://hdl.handle.net/10722/163447
dc.identifier.volume139
dc.languageeng
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
dc.publisher.placeIreland
dc.relation.ispartofJournal of Ethnopharmacology
dc.relation.referencesReferences in Scopus
dc.subject.meshAngiogenesis Inducing Agents - Pharmacology - Therapeutic Use - Toxicity
dc.subject.meshAngiogenic Proteins - Genetics
dc.subject.meshAnimals
dc.subject.meshAnimals, Genetically Modified
dc.subject.meshAorta - Drug Effects
dc.subject.meshCell Proliferation - Drug Effects
dc.subject.meshDiabetic Foot - Drug Therapy
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshDrugs, Chinese Herbal - Pharmacology - Therapeutic Use - Toxicity
dc.subject.meshEmbryo, Nonmammalian - Blood Supply - Drug Effects
dc.subject.meshEndothelial Cells - Drug Effects - Metabolism
dc.subject.meshG2 Phase Cell Cycle Checkpoints - Drug Effects
dc.subject.meshGene Expression Regulation - Drug Effects
dc.subject.meshHumans
dc.subject.meshLethal Dose 50
dc.subject.meshMap Kinase Signaling System - Drug Effects - Genetics
dc.subject.meshMale
dc.subject.meshNeovascularization, Physiologic - Drug Effects - Genetics
dc.subject.meshProto-Oncogene Protein C-Fli-1 - Genetics
dc.subject.meshRna, Messenger - Metabolism
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshReal-Time Polymerase Chain Reaction
dc.subject.meshS Phase Cell Cycle Checkpoints - Drug Effects
dc.subject.meshZebrafish - Embryology - Genetics
dc.titleAngiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong
  3. University of Macau