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Article: Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro
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TitleAngiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro
 
AuthorsTse, HYG3
Hui, MNY3
Li, L3
Lee, SMY2
Leung, AYH1
Cheng, SH3
 
Issue Date2012
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
 
CitationJournal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 447-453 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jep.2011.11.031
 
AbstractEthnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration. © 2011 Elsevier Ireland Ltd.
 
ISSN0378-8741
2013 Impact Factor: 2.939
2013 SCImago Journal Rankings: 1.149
 
DOIhttp://dx.doi.org/10.1016/j.jep.2011.11.031
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTse, HYG
 
dc.contributor.authorHui, MNY
 
dc.contributor.authorLi, L
 
dc.contributor.authorLee, SMY
 
dc.contributor.authorLeung, AYH
 
dc.contributor.authorCheng, SH
 
dc.date.accessioned2012-09-05T05:31:26Z
 
dc.date.available2012-09-05T05:31:26Z
 
dc.date.issued2012
 
dc.description.abstractEthnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration. © 2011 Elsevier Ireland Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Ethnopharmacology, 2012, v. 139 n. 2, p. 447-453 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.jep.2011.11.031
 
dc.identifier.citeulike10097018
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.jep.2011.11.031
 
dc.identifier.epage453
 
dc.identifier.issn0378-8741
2013 Impact Factor: 2.939
2013 SCImago Journal Rankings: 1.149
 
dc.identifier.issue2
 
dc.identifier.pmid22138660
 
dc.identifier.scopuseid_2-s2.0-84856006882
 
dc.identifier.spage447
 
dc.identifier.urihttp://hdl.handle.net/10722/163447
 
dc.identifier.volume139
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm
 
dc.publisher.placeIreland
 
dc.relation.ispartofJournal of Ethnopharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAngiogenesis Inducing Agents - Pharmacology - Therapeutic Use - Toxicity
 
dc.subject.meshAngiogenic Proteins - Genetics
 
dc.subject.meshAnimals
 
dc.subject.meshAnimals, Genetically Modified
 
dc.subject.meshAorta - Drug Effects
 
dc.subject.meshCell Proliferation - Drug Effects
 
dc.subject.meshDiabetic Foot - Drug Therapy
 
dc.subject.meshDose-Response Relationship, Drug
 
dc.subject.meshDrugs, Chinese Herbal - Pharmacology - Therapeutic Use - Toxicity
 
dc.subject.meshEmbryo, Nonmammalian - Blood Supply - Drug Effects
 
dc.subject.meshEndothelial Cells - Drug Effects - Metabolism
 
dc.subject.meshG2 Phase Cell Cycle Checkpoints - Drug Effects
 
dc.subject.meshGene Expression Regulation - Drug Effects
 
dc.subject.meshHumans
 
dc.subject.meshLethal Dose 50
 
dc.subject.meshMap Kinase Signaling System - Drug Effects - Genetics
 
dc.subject.meshMale
 
dc.subject.meshNeovascularization, Physiologic - Drug Effects - Genetics
 
dc.subject.meshProto-Oncogene Protein C-Fli-1 - Genetics
 
dc.subject.meshRna, Messenger - Metabolism
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshReal-Time Polymerase Chain Reaction
 
dc.subject.meshS Phase Cell Cycle Checkpoints - Drug Effects
 
dc.subject.meshZebrafish - Embryology - Genetics
 
dc.titleAngiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro
 
dc.typeArticle
 
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<contributor.author>Lee, SMY</contributor.author>
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<description.abstract>Ethnopharmacological relevance: Diabetic foot ulceration results in high risk of lower extremity amputation, and represents a significant health care expenditure worldwide. Radix Astragali (RA) and Radix Rehmanniae (RR) are widely used Chinese medicinal herbs in treating diabetes, and have shown positive effects in enhancing wound healing in diabetic foot ulcer animal model. Materials and methods: The angiogenic efficacy of NF3, a simplified 2-herb formula consisting of RA and RR in 2:1 ratio, was investigated. Median lethal concentration (LC50) and median effective concentration (EC50) were determined by treating zebrafish embryos with different concentrations of NF3 from 20 hpf to 72 hpf. The angiogenic activity of NF3 was examined in zebrafish embryos in vivo and by rat aortic ring assay in vitro. Cell cycle analysis of endothelial cells induced by NF3 was analyzed by flow cytometry using transgenic zebrafish Tg(fli1:EGFP). Real-time PCR was used to analyze mRNA expression profiles of selected genes involved in VEGF, FGF and MAPK pathways. Results: NF3 enhanced blood vessel formation as indicated by extra growth of intersegmental vessels in zebrafish embryos, and increased microvessels formation in rat aortic ring. NF3 also enhanced endothelial cells proliferation as shown by increased percentage of cells accumulating in S phase and G2/M phase of the cell cycle. NF3 exposure significantly induced up-regulation of VEGF-A, Flk-1, fgf1 and bRaf expression in zebrafish embryos. Conclusions: Our results demonstrated that NF3 was effective in promoting angiogenesis in zebrafish embryos and by rat aortic ring assay, which provided scientific basis to support the use of NF3 as potential therapeutics in treating diabetic foot ulceration. &#169; 2011 Elsevier Ireland Ltd.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. University of Macau
  3. City University of Hong Kong