File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers

TitleProbing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers
Authors
Issue Date2012
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomech
Citation
Journal Of Biomechanics, 2012, v. 45 n. 1, p. 123-128 How to Cite?
AbstractHuman embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and static mechanical responses of cells during optical stretching. Our experiments showed that the mechanical stiffness of differentiated hESC-CM increased after cardiac differentiation. Such stiffening could associate with increasingly organized myofibrillar assembly that underlines the functional characteristics of hESC-CM. In summary, our findings lay the ground work for using hESC-CMs as models to study mechanical and contractile defects in heart diseases. © 2011 Elsevier Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/163436
ISSN
2015 Impact Factor: 2.431
2015 SCImago Journal Rankings: 1.233
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTan, Yen_US
dc.contributor.authorKong, CWen_US
dc.contributor.authorChen, Sen_US
dc.contributor.authorCheng, SHen_US
dc.contributor.authorLi, RAen_US
dc.contributor.authorSun, Den_US
dc.date.accessioned2012-09-05T05:31:19Z-
dc.date.available2012-09-05T05:31:19Z-
dc.date.issued2012en_US
dc.identifier.citationJournal Of Biomechanics, 2012, v. 45 n. 1, p. 123-128en_US
dc.identifier.issn0021-9290en_US
dc.identifier.urihttp://hdl.handle.net/10722/163436-
dc.description.abstractHuman embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and static mechanical responses of cells during optical stretching. Our experiments showed that the mechanical stiffness of differentiated hESC-CM increased after cardiac differentiation. Such stiffening could associate with increasingly organized myofibrillar assembly that underlines the functional characteristics of hESC-CM. In summary, our findings lay the ground work for using hESC-CMs as models to study mechanical and contractile defects in heart diseases. © 2011 Elsevier Ltd.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomechen_US
dc.relation.ispartofJournal of Biomechanicsen_US
dc.subject.meshBiomechanics - Physiologyen_US
dc.subject.meshCardiovascular Diseases - Physiopathologyen_US
dc.subject.meshCell Differentiation - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEmbryonic Stem Cells - Cytology - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshMyocardial Contraction - Physiologyen_US
dc.subject.meshMyocytes, Cardiac - Cytology - Physiologyen_US
dc.subject.meshMyofibrils - Physiologyen_US
dc.subject.meshOptical Tweezersen_US
dc.titleProbing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezersen_US
dc.typeArticleen_US
dc.identifier.emailKong, CW:marcokong@hku.hken_US
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_US
dc.identifier.authorityKong, CW=rp01563en_US
dc.identifier.authorityLi, RA=rp01352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jbiomech.2011.09.007en_US
dc.identifier.pmid22104169-
dc.identifier.scopuseid_2-s2.0-83555178421en_US
dc.identifier.hkuros212204-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-83555178421&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume45en_US
dc.identifier.issue1en_US
dc.identifier.spage123en_US
dc.identifier.epage128en_US
dc.identifier.isiWOS:000299448500016-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTan, Y=26028707000en_US
dc.identifier.scopusauthoridKong, CW=36784634200en_US
dc.identifier.scopusauthoridChen, S=53986175500en_US
dc.identifier.scopusauthoridCheng, SH=20233852300en_US
dc.identifier.scopusauthoridLi, RA=7404724466en_US
dc.identifier.scopusauthoridSun, D=7403968591en_US
dc.identifier.citeulike10063807-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats