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Article: Cobalt chloride pretreatment promotes cardiac differentiation of human embryonic stem cells under atmospheric oxygen level

TitleCobalt chloride pretreatment promotes cardiac differentiation of human embryonic stem cells under atmospheric oxygen level
Authors
Issue Date2011
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/clo
Citation
Cellular Reprogramming, 2011, v. 13 n. 6, p. 527-537 How to Cite?
AbstractOur previous study demonstrated the direct involvement of the HIF-1α subunit in the promotion of cardiac differentiation of murine embryonic stem cells (ESCs). We report the use of cobalt chloride to induce HIF-1α stabilization in human ESCs to promote cardiac differentiation. Treatment of undifferentiated hES2 human ESCs with 50μM cobalt chloride markedly increased protein levels of the HIF-1α subunit, and was associated with increased expression of early cardiac specific transcription factors and cardiotrophic factors including NK2.5, vascular endothelial growth factor, and cardiotrophin-1. When pretreated cells were subjected to cardiac differentiation, a notable increase in the occurrence of beating embryoid bodies and sarcomeric actinin-positive cells was observed, along with increased expression of the cardiac-specific markers, MHC-A, MHC-B, and MLC2V. Electrophysiological study revealed increased atrial-and nodal-like cells in the cobalt chloride-pretreated group. Confocal calcium imaging analysis indicated that the maximum upstroke and decay velocities were significantly increased in both noncaffeine and caffeine-induced calcium transient in cardiomyocytes derived from the cobalt chloride-pretreated cells, suggesting these cells were functionally more mature. In conclusion, our study demonstrated that cobalt chloride pretreatment of hES2 human ESCs promotes cardiac differentiation and the maturation of calcium homeostasis of cardiomyocytes derived from ESCs. © 2011 Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/163432
ISSN
2015 Impact Factor: 1.462
2015 SCImago Journal Rankings: 0.681
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, KMen_HK
dc.contributor.authorChan, YCen_HK
dc.contributor.authorLee, YKen_HK
dc.contributor.authorLai, WHen_HK
dc.contributor.authorAu, KWen_HK
dc.contributor.authorFung, MLen_HK
dc.contributor.authorSiu, CWen_HK
dc.contributor.authorLi, RAen_HK
dc.contributor.authorTse, HFen_HK
dc.date.accessioned2012-09-05T05:31:17Z-
dc.date.available2012-09-05T05:31:17Z-
dc.date.issued2011en_HK
dc.identifier.citationCellular Reprogramming, 2011, v. 13 n. 6, p. 527-537en_HK
dc.identifier.issn2152-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/163432-
dc.description.abstractOur previous study demonstrated the direct involvement of the HIF-1α subunit in the promotion of cardiac differentiation of murine embryonic stem cells (ESCs). We report the use of cobalt chloride to induce HIF-1α stabilization in human ESCs to promote cardiac differentiation. Treatment of undifferentiated hES2 human ESCs with 50μM cobalt chloride markedly increased protein levels of the HIF-1α subunit, and was associated with increased expression of early cardiac specific transcription factors and cardiotrophic factors including NK2.5, vascular endothelial growth factor, and cardiotrophin-1. When pretreated cells were subjected to cardiac differentiation, a notable increase in the occurrence of beating embryoid bodies and sarcomeric actinin-positive cells was observed, along with increased expression of the cardiac-specific markers, MHC-A, MHC-B, and MLC2V. Electrophysiological study revealed increased atrial-and nodal-like cells in the cobalt chloride-pretreated group. Confocal calcium imaging analysis indicated that the maximum upstroke and decay velocities were significantly increased in both noncaffeine and caffeine-induced calcium transient in cardiomyocytes derived from the cobalt chloride-pretreated cells, suggesting these cells were functionally more mature. In conclusion, our study demonstrated that cobalt chloride pretreatment of hES2 human ESCs promotes cardiac differentiation and the maturation of calcium homeostasis of cardiomyocytes derived from ESCs. © 2011 Mary Ann Liebert, Inc.en_HK
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/cloen_HK
dc.relation.ispartofCellular Reprogrammingen_HK
dc.rightsThis is a copy of an article published in the Cellular Reprogramming © 2011 copyright Mary Ann Liebert, Inc.; Cellular Reprogramming is available online at: http://www.liebertonline.com-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Differentiation - Biosynthesisen_US
dc.subject.meshAntimutagenic Agents - Pharmacologyen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCobalt - Pharmacologyen_US
dc.subject.meshEmbryonic Stem Cells - Cytology - Metabolismen_US
dc.subject.meshHomeostasis - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMyocardium - Cytology - Metabolismen_US
dc.subject.meshOrgan Specificity - Drug Effectsen_US
dc.titleCobalt chloride pretreatment promotes cardiac differentiation of human embryonic stem cells under atmospheric oxygen levelen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, KM: skykmng@hkucc.hku.hken_HK
dc.identifier.emailChan, YC: ycchan09@hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.emailSiu, CW: cwdsiu@hkucc.hku.hken_HK
dc.identifier.emailLi, RA: ronaldli@hkucc.hku.hken_HK
dc.identifier.emailTse, HF: hftse@hkucc.hku.hken_HK
dc.identifier.authorityNg, KM=rp01670en_HK
dc.identifier.authorityChan, YC=rp01502en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.identifier.authoritySiu, CW=rp00534en_HK
dc.identifier.authorityLi, RA=rp01352en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1089/cell.2011.0038en_HK
dc.identifier.pmid22029419-
dc.identifier.scopuseid_2-s2.0-83255193347en_HK
dc.identifier.hkuros197119-
dc.identifier.hkuros209183-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-83255193347&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue6en_HK
dc.identifier.spage527en_HK
dc.identifier.epage537en_HK
dc.identifier.isiWOS:000298151100008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, KM=25122990200en_HK
dc.identifier.scopusauthoridChan, YC=7403676116en_HK
dc.identifier.scopusauthoridLee, YK=25958641200en_HK
dc.identifier.scopusauthoridLai, WH=18434390500en_HK
dc.identifier.scopusauthoridAu, KW=9738204200en_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK
dc.identifier.scopusauthoridSiu, CW=7006550690en_HK
dc.identifier.scopusauthoridLi, RA=7404724466en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK

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