File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Label-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy

TitleLabel-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopy
Authors
KeywordsCardiomyocytes
Cell Separation
Myosin
Pluripotent Stem Cells
Second Harmonic Generation
Issue Date2012
PublisherWiley - V C H Verlag GmbH & Co. KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/114172147
Citation
Journal Of Biophotonics, 2012, v. 5 n. 1, p. 57-66 How to Cite?
AbstractPluripotent stem cell-derived cardiomyocytes (PSC-CMs) are a potentially unlimited source of cardiomyocytes (CMs) for cardiac transplantation therapies. The establishment of pure PSC-CM populations is important for this application, but is hampered by a lack of CM-specific surface markers suitable for their identification and sorting. Contemporary purification techniques are either non-specific or require genetic modification. We report a second harmonic generation (SHG) signal detectable in PSC-CMs that is attributable to sarcomeric myosin, dependent on PSC-CM maturity, and retained while PSC-CMs are in suspension. Our study demonstrates the feasibility of developing a SHG-activated flow cytometer for the non-invasive purification of PSC-CMs. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/163431
ISSN
2015 Impact Factor: 3.818
2015 SCImago Journal Rankings: 1.204
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAwasthi, Sen_US
dc.contributor.authorMatthews, DLen_US
dc.contributor.authorLi, RAen_US
dc.contributor.authorChiamvimonvat, Nen_US
dc.contributor.authorLieu, DKen_US
dc.contributor.authorChan, JWen_US
dc.date.accessioned2012-09-05T05:31:17Z-
dc.date.available2012-09-05T05:31:17Z-
dc.date.issued2012en_US
dc.identifier.citationJournal Of Biophotonics, 2012, v. 5 n. 1, p. 57-66en_US
dc.identifier.issn1864-063Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/163431-
dc.description.abstractPluripotent stem cell-derived cardiomyocytes (PSC-CMs) are a potentially unlimited source of cardiomyocytes (CMs) for cardiac transplantation therapies. The establishment of pure PSC-CM populations is important for this application, but is hampered by a lack of CM-specific surface markers suitable for their identification and sorting. Contemporary purification techniques are either non-specific or require genetic modification. We report a second harmonic generation (SHG) signal detectable in PSC-CMs that is attributable to sarcomeric myosin, dependent on PSC-CM maturity, and retained while PSC-CMs are in suspension. Our study demonstrates the feasibility of developing a SHG-activated flow cytometer for the non-invasive purification of PSC-CMs. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co. KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/114172147en_US
dc.relation.ispartofJournal of Biophotonicsen_US
dc.subjectCardiomyocytesen_US
dc.subjectCell Separationen_US
dc.subjectMyosinen_US
dc.subjectPluripotent Stem Cellsen_US
dc.subjectSecond Harmonic Generationen_US
dc.titleLabel-free identification and characterization of human pluripotent stem cell-derived cardiomyocytes using second harmonic generation (SHG) microscopyen_US
dc.typeArticleen_US
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_US
dc.identifier.authorityLi, RA=rp01352en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1002/jbio.201100077en_US
dc.identifier.pmid22083829-
dc.identifier.scopuseid_2-s2.0-83155184658en_US
dc.identifier.hkuros197111-
dc.identifier.hkuros239707-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-83155184658&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume5en_US
dc.identifier.issue1en_US
dc.identifier.spage57en_US
dc.identifier.epage66en_US
dc.identifier.isiWOS:000297850900009-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridAwasthi, S=54793960900en_US
dc.identifier.scopusauthoridMatthews, DL=7402357233en_US
dc.identifier.scopusauthoridLi, RA=7404724466en_US
dc.identifier.scopusauthoridChiamvimonvat, N=7004461965en_US
dc.identifier.scopusauthoridLieu, DK=7003924538en_US
dc.identifier.scopusauthoridChan, JW=26432534400en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats