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Article: [Serum of obesity induce the activation of TLR4/NF-κB signaling pathway on THP-1 cell line].

Title[Serum of obesity induce the activation of TLR4/NF-κB signaling pathway on THP-1 cell line].
Authors
Issue Date2010
Publisher中華醫學會. The Journal's web site is located at http://zhyx.chinajournal.net.cn/
Citation
Zhonghua Yi Xue Za Zhi, 2010, v. 90 n. 44, p. 3119-3123 How to Cite?
AbstractTo investigate the activation of TLR4/NF-κB signaling pathway and the level of inflammation in volunteers with varying degrees of metabolic disorders and the effect of intervention by TLR4 monoclonal antibody. THP-1 cell line was cultured with 10% serum of volunteers with various degrees of metabolic disorders (each 10 cases) for 24, 48 h. TLR4 and NF-κB p65 phosphorylation protein were measured by Western blot. TLR4 mRNA was measured by RT-PCR. The expression of IL-1β and TNF-α were detected by ELISA. The TLR4 mRNA and protein, the level of NF-κB p65 phosphorylation protein in THP-1 cell line and IL-1β and TNF-α expression in culture supernatant in normal, simple obesity, obesity with hyperglycemia, obesity with hyperlipidemia, obesity with three metabolic disorders groups had statistical significance (P < 0.05). The TLR4 mRNA and protein, the level of NF-κB p65 phosphorylation protein and the IL-1β and TNF-α expression in obesity with three metabolic disorders group were higher than those in other group, and those were time-dependent (P < 0.05). TNF-α expression in normal, simple obesity, obesity with hyperglycemia, obesity with hyperlipidemia, obesity with three metabolic disorders group at 48 h were (222 ± 32), (246 ± 52), (322 ± 32), (322 ± 34) and(490 ± 83)ng/L, respectively, IL-1β (ng/L) were (94 ± 19), (133 ± 19), (174 ± 22), (180 ± 30), (279 ± 38) (P < 0.05). The activation of TLR4/NF-κB signaling pathway on THP-1 cell line which is cultured by serum with various degrees of metabolic disorders is different, with the increasing of severity of metabolic disorders, TLR4/NF-κB signaling pathway activation levels also incr.
Persistent Identifierhttp://hdl.handle.net/10722/163398
ISSN
2015 SCImago Journal Rankings: 0.134

 

DC FieldValueLanguage
dc.contributor.authorYao, Len_US
dc.contributor.authorXiao, Yen_US
dc.contributor.authorLiu, SPen_US
dc.contributor.authorXu, AMen_US
dc.contributor.authorZhou, ZGen_US
dc.date.accessioned2012-09-05T05:30:53Z-
dc.date.available2012-09-05T05:30:53Z-
dc.date.issued2010en_US
dc.identifier.citationZhonghua Yi Xue Za Zhi, 2010, v. 90 n. 44, p. 3119-3123en_US
dc.identifier.issn0376-2491en_US
dc.identifier.urihttp://hdl.handle.net/10722/163398-
dc.description.abstractTo investigate the activation of TLR4/NF-κB signaling pathway and the level of inflammation in volunteers with varying degrees of metabolic disorders and the effect of intervention by TLR4 monoclonal antibody. THP-1 cell line was cultured with 10% serum of volunteers with various degrees of metabolic disorders (each 10 cases) for 24, 48 h. TLR4 and NF-κB p65 phosphorylation protein were measured by Western blot. TLR4 mRNA was measured by RT-PCR. The expression of IL-1β and TNF-α were detected by ELISA. The TLR4 mRNA and protein, the level of NF-κB p65 phosphorylation protein in THP-1 cell line and IL-1β and TNF-α expression in culture supernatant in normal, simple obesity, obesity with hyperglycemia, obesity with hyperlipidemia, obesity with three metabolic disorders groups had statistical significance (P < 0.05). The TLR4 mRNA and protein, the level of NF-κB p65 phosphorylation protein and the IL-1β and TNF-α expression in obesity with three metabolic disorders group were higher than those in other group, and those were time-dependent (P < 0.05). TNF-α expression in normal, simple obesity, obesity with hyperglycemia, obesity with hyperlipidemia, obesity with three metabolic disorders group at 48 h were (222 ± 32), (246 ± 52), (322 ± 32), (322 ± 34) and(490 ± 83)ng/L, respectively, IL-1β (ng/L) were (94 ± 19), (133 ± 19), (174 ± 22), (180 ± 30), (279 ± 38) (P < 0.05). The activation of TLR4/NF-κB signaling pathway on THP-1 cell line which is cultured by serum with various degrees of metabolic disorders is different, with the increasing of severity of metabolic disorders, TLR4/NF-κB signaling pathway activation levels also incr.en_US
dc.languageengen_US
dc.publisher中華醫學會. The Journal's web site is located at http://zhyx.chinajournal.net.cn/en_US
dc.relation.ispartofZhonghua yi xue za zhien_US
dc.subject.meshAdulten_US
dc.subject.meshCell Lineen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshInflammationen_US
dc.subject.meshInterleukin-1Beta - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMetabolic Syndrome X - Metabolismen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMonocytes - Metabolismen_US
dc.subject.meshObesity - Blooden_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshSerumen_US
dc.subject.meshSignal Transductionen_US
dc.subject.meshToll-Like Receptor 4 - Metabolismen_US
dc.subject.meshTranscription Factor Rela - Metabolismen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Metabolismen_US
dc.title[Serum of obesity induce the activation of TLR4/NF-κB signaling pathway on THP-1 cell line].en_US
dc.typeArticleen_US
dc.identifier.emailXu, AM:amxu@hkucc.hku.hken_US
dc.identifier.authorityXu, AM=rp00485en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid21211341-
dc.identifier.scopuseid_2-s2.0-80052797829en_US
dc.identifier.volume90en_US
dc.identifier.issue44en_US
dc.identifier.spage3119en_US
dc.identifier.epage3123en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridYao, L=36103474800en_US
dc.identifier.scopusauthoridXiao, Y=36464707600en_US
dc.identifier.scopusauthoridLiu, SP=50361659300en_US
dc.identifier.scopusauthoridXu, AM=7202655409en_US
dc.identifier.scopusauthoridZhou, ZG=50362347700en_US

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