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Article: Manipulations of microRNA in human pluripotent stem cells and their derivatives

TitleManipulations of microRNA in human pluripotent stem cells and their derivatives
Authors
KeywordsDirected Differentiation
Human Embryonic Stem Cells
Microrna
Rna Interference
Issue Date2011
Citation
Methods In Molecular Biology, 2011, v. 690, p. 107-120 How to Cite?
AbstractHuman embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve as excellent in vitro models for the study of human organogenesis and disease models, as well as drug toxicity screening. MicroRNAs (miRNAs) are nonencoding RNAs of ∼22 nucleotides that function as negative transcriptional regulators via degradation or inhibition by RNA interference (RNAi). MiRNAs play essential roles in developmental pathways. This chapter provides a description of how miRNAs can be introduced into hESCs/iPSCs or their derivatives for experiments via lentivirus-mediated gene transfer. © 2011 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/163367
ISSN
2015 SCImago Journal Rankings: 0.549
References

 

DC FieldValueLanguage
dc.contributor.authorRushing, SNen_US
dc.contributor.authorHerren, AWen_US
dc.contributor.authorLieu, DKen_US
dc.contributor.authorLi, RAen_US
dc.date.accessioned2012-09-05T05:30:37Z-
dc.date.available2012-09-05T05:30:37Z-
dc.date.issued2011en_US
dc.identifier.citationMethods In Molecular Biology, 2011, v. 690, p. 107-120en_US
dc.identifier.issn1064-3745en_US
dc.identifier.urihttp://hdl.handle.net/10722/163367-
dc.description.abstractHuman embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve as excellent in vitro models for the study of human organogenesis and disease models, as well as drug toxicity screening. MicroRNAs (miRNAs) are nonencoding RNAs of ∼22 nucleotides that function as negative transcriptional regulators via degradation or inhibition by RNA interference (RNAi). MiRNAs play essential roles in developmental pathways. This chapter provides a description of how miRNAs can be introduced into hESCs/iPSCs or their derivatives for experiments via lentivirus-mediated gene transfer. © 2011 Springer Science+Business Media, LLC.en_US
dc.languageengen_US
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.subjectDirected Differentiationen_US
dc.subjectHuman Embryonic Stem Cellsen_US
dc.subjectMicrornaen_US
dc.subjectRna Interferenceen_US
dc.titleManipulations of microRNA in human pluripotent stem cells and their derivativesen_US
dc.typeArticleen_US
dc.identifier.emailLi, RA:ronaldli@hkucc.hku.hken_US
dc.identifier.authorityLi, RA=rp01352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/978-1-60761-962-8_7en_US
dc.identifier.scopuseid_2-s2.0-79952202114en_US
dc.identifier.hkuros239700-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952202114&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume690en_US
dc.identifier.spage107en_US
dc.identifier.epage120en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridRushing, SN=25121769500en_US
dc.identifier.scopusauthoridHerren, AW=37004474100en_US
dc.identifier.scopusauthoridLieu, DK=7003924538en_US
dc.identifier.scopusauthoridLi, RA=7404724466en_US

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