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Article: A DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryos

TitleA DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryos
Authors
Issue Date2010
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
Citation
Leukemia, 2010, v. 24 n. 12, p. 2090-2099 How to Cite?
AbstractAlthough aldehyde dehydrogenase (ALDH) activity has become a surrogate of hematopoietic stem and progenitor cells (HSPCs), its function during hematopoiesis was unclear. Here, we examined its role in zebrafish hematopoiesis based on pharmacological inhibition and morpholino (MO) knockdown. Zebrafish embryos were treated with diethylaminobenzaldehyde (DEAB, 1 mol/l) between 0- and 48 hour-post-fertilization (hpf). MOs targeting aldhs were injected between 1 and 4-cell stage. The effects on hematopoiesis were evaluated at different stages. DEAB treatment between 0 and 18 hpf increased gene expression associated with HSPC (scl, lmo2), erythropoiesis (gata1, α- and Β-eHb) and myelopoiesis (spi1) as well as gfp cells in dissociated Tg(gata1:gfp) embryos. The effects were ameliorated by all-trans retinoic acid (1 nmol/l). Definitive hematopoiesis and the erythromyeloid precursors were unaffected. In all, 14 out of 15 zebrafish aldhs were detectable by reverse transcription PCR in 18 hpf embryos, of which only aldh1a2 and aldh16a1 were expressed in sites pertinent to hematopoiesis. Molecular targeting by MOs was demonstrated for 15 aldhs, but none of them, even in combined aldh1a2 and aldh1a3 knockdown, recapitulated the hematopoietic expansion in DEAB-treated embryos. In conclusion, DEAB expands HSPC population during primitive hematopoiesis through inhibition of aldh and retinoic acid synthesis. The specific aldh isoform(s) remains to be determined. © 2010 Macmillan Publishers Limited All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/163350
ISSN
2014 Impact Factor: 10.431
2013 SCImago Journal Rankings: 4.500
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, ACHen_US
dc.contributor.authorChung, MISen_US
dc.contributor.authorLiang, Ren_US
dc.contributor.authorLeung, AYHen_US
dc.date.accessioned2012-09-05T05:30:25Z-
dc.date.available2012-09-05T05:30:25Z-
dc.date.issued2010en_US
dc.identifier.citationLeukemia, 2010, v. 24 n. 12, p. 2090-2099en_US
dc.identifier.issn0887-6924en_US
dc.identifier.urihttp://hdl.handle.net/10722/163350-
dc.description.abstractAlthough aldehyde dehydrogenase (ALDH) activity has become a surrogate of hematopoietic stem and progenitor cells (HSPCs), its function during hematopoiesis was unclear. Here, we examined its role in zebrafish hematopoiesis based on pharmacological inhibition and morpholino (MO) knockdown. Zebrafish embryos were treated with diethylaminobenzaldehyde (DEAB, 1 mol/l) between 0- and 48 hour-post-fertilization (hpf). MOs targeting aldhs were injected between 1 and 4-cell stage. The effects on hematopoiesis were evaluated at different stages. DEAB treatment between 0 and 18 hpf increased gene expression associated with HSPC (scl, lmo2), erythropoiesis (gata1, α- and Β-eHb) and myelopoiesis (spi1) as well as gfp cells in dissociated Tg(gata1:gfp) embryos. The effects were ameliorated by all-trans retinoic acid (1 nmol/l). Definitive hematopoiesis and the erythromyeloid precursors were unaffected. In all, 14 out of 15 zebrafish aldhs were detectable by reverse transcription PCR in 18 hpf embryos, of which only aldh1a2 and aldh16a1 were expressed in sites pertinent to hematopoiesis. Molecular targeting by MOs was demonstrated for 15 aldhs, but none of them, even in combined aldh1a2 and aldh1a3 knockdown, recapitulated the hematopoietic expansion in DEAB-treated embryos. In conclusion, DEAB expands HSPC population during primitive hematopoiesis through inhibition of aldh and retinoic acid synthesis. The specific aldh isoform(s) remains to be determined. © 2010 Macmillan Publishers Limited All rights reserved.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leuen_US
dc.relation.ispartofLeukemiaen_US
dc.subject.meshAldehyde Dehydrogenase - Antagonists & Inhibitors - Physiologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulation, Developmental - Drug Effectsen_US
dc.subject.meshHematopoiesis - Drug Effectsen_US
dc.subject.meshHematopoietic Stem Cells - Drug Effects - Physiologyen_US
dc.subject.meshTretinoin - Pharmacologyen_US
dc.subject.meshZebrafish - Embryologyen_US
dc.titleA DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryosen_US
dc.typeArticleen_US
dc.identifier.emailLiang, R:rliang@hku.hken_US
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_US
dc.identifier.authorityLiang, R=rp00345en_US
dc.identifier.authorityLeung, AYH=rp00265en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/leu.2010.206en_US
dc.identifier.pmid20927131en_US
dc.identifier.scopuseid_2-s2.0-78650304777en_US
dc.identifier.hkuros183884-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650304777&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume24en_US
dc.identifier.issue12en_US
dc.identifier.spage2090en_US
dc.identifier.epage2099en_US
dc.identifier.eissn1476-5551-
dc.identifier.isiWOS:000285380900014-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridMa, ACH=15849157500en_US
dc.identifier.scopusauthoridChung, MIS=25958659100en_US
dc.identifier.scopusauthoridLiang, R=26643224900en_US
dc.identifier.scopusauthoridLeung, AYH=7403012668en_US
dc.identifier.citeulike7967680-

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