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Article: A DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryos
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TitleA DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryos
 
AuthorsMa, ACH1
Chung, MIS1
Liang, R1
Leung, AYH1
 
Issue Date2010
 
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
 
CitationLeukemia, 2010, v. 24 n. 12, p. 2090-2099 [How to Cite?]
DOI: http://dx.doi.org/10.1038/leu.2010.206
 
AbstractAlthough aldehyde dehydrogenase (ALDH) activity has become a surrogate of hematopoietic stem and progenitor cells (HSPCs), its function during hematopoiesis was unclear. Here, we examined its role in zebrafish hematopoiesis based on pharmacological inhibition and morpholino (MO) knockdown. Zebrafish embryos were treated with diethylaminobenzaldehyde (DEAB, 1 mol/l) between 0- and 48 hour-post-fertilization (hpf). MOs targeting aldhs were injected between 1 and 4-cell stage. The effects on hematopoiesis were evaluated at different stages. DEAB treatment between 0 and 18 hpf increased gene expression associated with HSPC (scl, lmo2), erythropoiesis (gata1, α- and Β-eHb) and myelopoiesis (spi1) as well as gfp cells in dissociated Tg(gata1:gfp) embryos. The effects were ameliorated by all-trans retinoic acid (1 nmol/l). Definitive hematopoiesis and the erythromyeloid precursors were unaffected. In all, 14 out of 15 zebrafish aldhs were detectable by reverse transcription PCR in 18 hpf embryos, of which only aldh1a2 and aldh16a1 were expressed in sites pertinent to hematopoiesis. Molecular targeting by MOs was demonstrated for 15 aldhs, but none of them, even in combined aldh1a2 and aldh1a3 knockdown, recapitulated the hematopoietic expansion in DEAB-treated embryos. In conclusion, DEAB expands HSPC population during primitive hematopoiesis through inhibition of aldh and retinoic acid synthesis. The specific aldh isoform(s) remains to be determined. © 2010 Macmillan Publishers Limited All rights reserved.
 
ISSN0887-6924
2012 Impact Factor: 10.164
2012 SCImago Journal Rankings: 3.379
 
DOIhttp://dx.doi.org/10.1038/leu.2010.206
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorMa, ACH
 
dc.contributor.authorChung, MIS
 
dc.contributor.authorLiang, R
 
dc.contributor.authorLeung, AYH
 
dc.date.accessioned2012-09-05T05:30:25Z
 
dc.date.available2012-09-05T05:30:25Z
 
dc.date.issued2010
 
dc.description.abstractAlthough aldehyde dehydrogenase (ALDH) activity has become a surrogate of hematopoietic stem and progenitor cells (HSPCs), its function during hematopoiesis was unclear. Here, we examined its role in zebrafish hematopoiesis based on pharmacological inhibition and morpholino (MO) knockdown. Zebrafish embryos were treated with diethylaminobenzaldehyde (DEAB, 1 mol/l) between 0- and 48 hour-post-fertilization (hpf). MOs targeting aldhs were injected between 1 and 4-cell stage. The effects on hematopoiesis were evaluated at different stages. DEAB treatment between 0 and 18 hpf increased gene expression associated with HSPC (scl, lmo2), erythropoiesis (gata1, α- and Β-eHb) and myelopoiesis (spi1) as well as gfp cells in dissociated Tg(gata1:gfp) embryos. The effects were ameliorated by all-trans retinoic acid (1 nmol/l). Definitive hematopoiesis and the erythromyeloid precursors were unaffected. In all, 14 out of 15 zebrafish aldhs were detectable by reverse transcription PCR in 18 hpf embryos, of which only aldh1a2 and aldh16a1 were expressed in sites pertinent to hematopoiesis. Molecular targeting by MOs was demonstrated for 15 aldhs, but none of them, even in combined aldh1a2 and aldh1a3 knockdown, recapitulated the hematopoietic expansion in DEAB-treated embryos. In conclusion, DEAB expands HSPC population during primitive hematopoiesis through inhibition of aldh and retinoic acid synthesis. The specific aldh isoform(s) remains to be determined. © 2010 Macmillan Publishers Limited All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationLeukemia, 2010, v. 24 n. 12, p. 2090-2099 [How to Cite?]
DOI: http://dx.doi.org/10.1038/leu.2010.206
 
dc.identifier.citeulike7967680
 
dc.identifier.doihttp://dx.doi.org/10.1038/leu.2010.206
 
dc.identifier.eissn1476-5551
 
dc.identifier.epage2099
 
dc.identifier.issn0887-6924
2012 Impact Factor: 10.164
2012 SCImago Journal Rankings: 3.379
 
dc.identifier.issue12
 
dc.identifier.pmid20927131
 
dc.identifier.scopuseid_2-s2.0-78650304777
 
dc.identifier.spage2090
 
dc.identifier.urihttp://hdl.handle.net/10722/163350
 
dc.identifier.volume24
 
dc.languageeng
 
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofLeukemia
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAldehyde Dehydrogenase - Antagonists & Inhibitors - Physiology
 
dc.subject.meshAnimals
 
dc.subject.meshCell Differentiation - Drug Effects
 
dc.subject.meshEnzyme Inhibitors - Pharmacology
 
dc.subject.meshGene Expression Profiling
 
dc.subject.meshGene Expression Regulation, Developmental - Drug Effects
 
dc.subject.meshHematopoiesis - Drug Effects
 
dc.subject.meshHematopoietic Stem Cells - Drug Effects - Physiology
 
dc.subject.meshTretinoin - Pharmacology
 
dc.subject.meshZebrafish - Embryology
 
dc.titleA DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryos
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong