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Article: Gene expression profile in colon cancer cells with respect to XIAP expression status

TitleGene expression profile in colon cancer cells with respect to XIAP expression status
Authors
KeywordsAngiogenesis
Apoptosis
Colon cancer
Geneexpression
Microarray
PPARγ
Proliferation
XIAP
Issue Date2009
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00384/index.htm
Citation
International Journal Of Colorectal Disease, 2009, v. 24 n. 3, p. 245-260 How to Cite?
AbstractBackground and aims: We observed a marked synergism between peroxisome proliferator-activated receptor gamma (PPARγ) ligands and X-linked inhibitor of apoptosis (XIAP) down-regulation in colon cancer. In the current study, we detected the gene expression profile in HCT116 cells treated with or without PPARγ ligand troglitazone. Materials and methods: HCT116-XIAP +/+ and HCT116-XIAP -/- cells were treated with or without 50 μM troglitazone for 48 h. Gene expressions were detected by microarray, and selected genes were validated by reverse-transcriptase polymerase chain reaction (PCR), real-time PCR, and Western blot. Results: Relative to HCT116-XIAP +/+ cells, 58 genes were up-regulated and 33 genes down-regulated in HCT116-XIAP -/- cells, all by ≥4-fold. These genes could be classified into a wide variety of functional classes, but we focused on those related to angiogenesis, apoptosis, and proliferation. Thus, two pro-apoptotic genes and one pro-proliferation gene were up-regulated in HCT116-XIAP -/- cells. Two pro-proliferation genes, one pro-angiogenesis gene, one anti-angiogenesis gene, and one anti-apoptosis gene were down-regulated in HCT116-XIAP -/- cells. Relative to HCT116-XIAP +/+ cells treated with troglitazone, 137 genes were up-regulated, and 31 genes were down-regulated in troglitazone-treated HCT116-XIAP -/- cells, all by ≥4-fold. Among the up-regulated genes were two anti-angiogenesis genes, seven pro-apoptosis genes, and six anti-proliferation genes. Among the down-regulated genes were one anti-angiogenesis gene, one pro-angiogenesis gene, one anti-apoptosis gene, one anti-proliferation gene, and two pro-proliferation genes. Conclusion: Down-regulation of XIAP in HCT116 cells with or without troglitazone treatment was associated with changes of gene expression that favor increased tendency of apoptosis, decreased cell proliferation, and angiogenesis potential. © Springer-Verlag 2008.
Persistent Identifierhttp://hdl.handle.net/10722/163225
ISSN
2015 Impact Factor: 2.383
2015 SCImago Journal Rankings: 1.102
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQiao, Len_HK
dc.contributor.authorLi, GHYen_HK
dc.contributor.authorDai, Yen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorZou, Ben_HK
dc.contributor.authorGu, Qen_HK
dc.contributor.authorMa, Jen_HK
dc.contributor.authorPang, Ren_HK
dc.contributor.authorLan, HYen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2012-09-05T05:28:55Z-
dc.date.available2012-09-05T05:28:55Z-
dc.date.issued2009en_HK
dc.identifier.citationInternational Journal Of Colorectal Disease, 2009, v. 24 n. 3, p. 245-260en_HK
dc.identifier.issn0179-1958en_HK
dc.identifier.urihttp://hdl.handle.net/10722/163225-
dc.description.abstractBackground and aims: We observed a marked synergism between peroxisome proliferator-activated receptor gamma (PPARγ) ligands and X-linked inhibitor of apoptosis (XIAP) down-regulation in colon cancer. In the current study, we detected the gene expression profile in HCT116 cells treated with or without PPARγ ligand troglitazone. Materials and methods: HCT116-XIAP +/+ and HCT116-XIAP -/- cells were treated with or without 50 μM troglitazone for 48 h. Gene expressions were detected by microarray, and selected genes were validated by reverse-transcriptase polymerase chain reaction (PCR), real-time PCR, and Western blot. Results: Relative to HCT116-XIAP +/+ cells, 58 genes were up-regulated and 33 genes down-regulated in HCT116-XIAP -/- cells, all by ≥4-fold. These genes could be classified into a wide variety of functional classes, but we focused on those related to angiogenesis, apoptosis, and proliferation. Thus, two pro-apoptotic genes and one pro-proliferation gene were up-regulated in HCT116-XIAP -/- cells. Two pro-proliferation genes, one pro-angiogenesis gene, one anti-angiogenesis gene, and one anti-apoptosis gene were down-regulated in HCT116-XIAP -/- cells. Relative to HCT116-XIAP +/+ cells treated with troglitazone, 137 genes were up-regulated, and 31 genes were down-regulated in troglitazone-treated HCT116-XIAP -/- cells, all by ≥4-fold. Among the up-regulated genes were two anti-angiogenesis genes, seven pro-apoptosis genes, and six anti-proliferation genes. Among the down-regulated genes were one anti-angiogenesis gene, one pro-angiogenesis gene, one anti-apoptosis gene, one anti-proliferation gene, and two pro-proliferation genes. Conclusion: Down-regulation of XIAP in HCT116 cells with or without troglitazone treatment was associated with changes of gene expression that favor increased tendency of apoptosis, decreased cell proliferation, and angiogenesis potential. © Springer-Verlag 2008.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00384/index.htmen_HK
dc.relation.ispartofInternational Journal of Colorectal Diseaseen_HK
dc.subjectAngiogenesisen_HK
dc.subjectApoptosisen_HK
dc.subjectColon canceren_HK
dc.subjectGeneexpressionen_HK
dc.subjectMicroarrayen_HK
dc.subjectPPARγen_HK
dc.subjectProliferationen_HK
dc.subjectXIAPen_HK
dc.subject.meshApoptosis - Drug Effects - Geneticsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshChromans - Pharmacologyen_US
dc.subject.meshColonic Neoplasms - Genetics - Pathologyen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulation, Neoplastic - Drug Effectsen_US
dc.subject.meshHct116 Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshNeovascularization, Pathologic - Geneticsen_US
dc.subject.meshOligonucleotide Array Sequence Analysisen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshThiazolidinediones - Pharmacologyen_US
dc.subject.meshX-Linked Inhibitor Of Apoptosis Protein - Genetics - Metabolismen_US
dc.titleGene expression profile in colon cancer cells with respect to XIAP expression statusen_HK
dc.typeArticleen_HK
dc.identifier.emailQiao, L: lq8688@hotmail.comen_HK
dc.identifier.emailWang, J: jidewang@gmail.comen_HK
dc.identifier.emailPang, R: robertap@hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityQiao, L=rp00513en_HK
dc.identifier.authorityWang, J=rp00491en_HK
dc.identifier.authorityPang, R=rp00274en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00384-008-0566-1en_HK
dc.identifier.pmid18704457-
dc.identifier.scopuseid_2-s2.0-59649112825en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-59649112825&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue3en_HK
dc.identifier.spage245en_HK
dc.identifier.epage260en_HK
dc.identifier.isiWOS:000262986000001-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridQiao, L=7202151719en_HK
dc.identifier.scopusauthoridLi, GHY=35080710200en_HK
dc.identifier.scopusauthoridDai, Y=7401512993en_HK
dc.identifier.scopusauthoridWang, J=35309087500en_HK
dc.identifier.scopusauthoridLi, Z=24171072000en_HK
dc.identifier.scopusauthoridZou, B=35228257300en_HK
dc.identifier.scopusauthoridGu, Q=24469982400en_HK
dc.identifier.scopusauthoridMa, J=35275386200en_HK
dc.identifier.scopusauthoridPang, R=7004376659en_HK
dc.identifier.scopusauthoridLan, HY=7102710832en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.citeulike4162296-

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