File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Genetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA Met and AUG selection

TitleGenetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA Met and AUG selection
Authors
Issue Date2008
PublisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/
Citation
Genes And Development, 2008, v. 22 n. 16, p. 2242-2255 How to Cite?
AbstractHigh-resolution structures of bacterial 70S ribosomes have provided atomic details about mRNA and tRNA binding to the decoding center during elongation, but such information is lacking for preinitiation complexes (PICs). We identified residues in yeast 18S rRNA critical in vivo for recruiting methionyl tRNA i Met to 40S subunits during initiation by isolating mutations that derepress GCN4 mRNA translation. Several such Gcd - mutations alter the A928:U1389 base pair in helix 28 (h28) and allow PICs to scan through the start codons of upstream ORFs that normally repress GCN4 translation. The A928U substitution also impairs TC binding to PICs in a reconstituted system in vitro. Mutation of the bulge G926 in h28 and certain other residues corresponding to direct contacts with the P-site codon or tRNA in bacterial 70S complexes confer Gcd - phenotypes that (like A928 substitutions) are suppressed by overexpressing tRNA i Met. Hence, the nonconserved 928:1389 base pair in h28, plus conserved 18S rRNA residues corresponding to P-site contacts in bacterial ribosomes, are critical for efficient Met-tRNA i Met binding and AUG selection in eukaryotes. © 2008 Cold Spring Harbor Laboratory Press.
Persistent Identifierhttp://hdl.handle.net/10722/163197
ISSN
2015 Impact Factor: 10.042
2015 SCImago Journal Rankings: 11.812
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDong, Jen_US
dc.contributor.authorNanda, JSen_US
dc.contributor.authorRahman, Hen_US
dc.contributor.authorPruitt, MRen_US
dc.contributor.authorShin, BSen_US
dc.contributor.authorWong, CMen_US
dc.contributor.authorLorsch, JRen_US
dc.contributor.authorHinnebusch, AGen_US
dc.date.accessioned2012-09-05T05:28:38Z-
dc.date.available2012-09-05T05:28:38Z-
dc.date.issued2008en_US
dc.identifier.citationGenes And Development, 2008, v. 22 n. 16, p. 2242-2255en_US
dc.identifier.issn0890-9369en_US
dc.identifier.urihttp://hdl.handle.net/10722/163197-
dc.description.abstractHigh-resolution structures of bacterial 70S ribosomes have provided atomic details about mRNA and tRNA binding to the decoding center during elongation, but such information is lacking for preinitiation complexes (PICs). We identified residues in yeast 18S rRNA critical in vivo for recruiting methionyl tRNA i Met to 40S subunits during initiation by isolating mutations that derepress GCN4 mRNA translation. Several such Gcd - mutations alter the A928:U1389 base pair in helix 28 (h28) and allow PICs to scan through the start codons of upstream ORFs that normally repress GCN4 translation. The A928U substitution also impairs TC binding to PICs in a reconstituted system in vitro. Mutation of the bulge G926 in h28 and certain other residues corresponding to direct contacts with the P-site codon or tRNA in bacterial 70S complexes confer Gcd - phenotypes that (like A928 substitutions) are suppressed by overexpressing tRNA i Met. Hence, the nonconserved 928:1389 base pair in h28, plus conserved 18S rRNA residues corresponding to P-site contacts in bacterial ribosomes, are critical for efficient Met-tRNA i Met binding and AUG selection in eukaryotes. © 2008 Cold Spring Harbor Laboratory Press.en_US
dc.languageengen_US
dc.publisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/en_US
dc.relation.ispartofGenes and Developmenten_US
dc.subject.meshAmino Acid Substitutionen_US
dc.subject.meshCodon, Initiator - Geneticsen_US
dc.subject.meshEukaryotic Initiation Factor-2B - Genetics - Metabolismen_US
dc.subject.meshMutation - Geneticsen_US
dc.subject.meshNucleic Acid Conformationen_US
dc.subject.meshOpen Reading Framesen_US
dc.subject.meshPeptide Chain Initiation, Translationalen_US
dc.subject.meshRna, Fungal - Genetics - Metabolismen_US
dc.subject.meshRna, Ribosomal, 18S - Geneticsen_US
dc.subject.meshRna, Transfer, Met - Geneticsen_US
dc.subject.meshSaccharomyces Cerevisiae - Geneticsen_US
dc.subject.meshSaccharomyces Cerevisiae Proteins - Genetics - Metabolismen_US
dc.titleGenetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA Met and AUG selectionen_US
dc.typeArticleen_US
dc.identifier.emailWong, CM:wispwong@hkucc.hku.hken_US
dc.identifier.authorityWong, CM=rp01489en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1101/gad.1696608en_US
dc.identifier.pmid18708582-
dc.identifier.pmcidPMC2518818-
dc.identifier.scopuseid_2-s2.0-50049130690en_US
dc.identifier.hkuros164802-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-50049130690&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume22en_US
dc.identifier.issue16en_US
dc.identifier.spage2242en_US
dc.identifier.epage2255en_US
dc.identifier.eissn1549-5477-
dc.identifier.isiWOS:000258486800010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDong, J=7403365571en_US
dc.identifier.scopusauthoridNanda, JS=23474796100en_US
dc.identifier.scopusauthoridRahman, H=24721745600en_US
dc.identifier.scopusauthoridPruitt, MR=24721854900en_US
dc.identifier.scopusauthoridShin, BS=7103027744en_US
dc.identifier.scopusauthoridWong, CM=18134632400en_US
dc.identifier.scopusauthoridLorsch, JR=6701446975en_US
dc.identifier.scopusauthoridHinnebusch, AG=7005728566en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats