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Article: Pancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer
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TitlePancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer
 
AuthorsMa, J2 1
Chen, M2
Wang, J1
Xia, HHX1
Zhu, S2
Liang, Y2
Gu, Q1
Qiao, L1
Dai, Y1
Zou, B1
Li, Z1
Zhang, Y1
Lan, H1
Wong, BCY1
 
Issue Date2008
 
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
CitationCarcinogenesis, 2008, v. 29 n. 7, p. 1327-1333 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/bgn112
 
AbstractAim: Pancreatic duodenal homeobox-1 (PDX1) is a transcription factor of homeobox genes family important in differentiation and development of the pancreas, duodenum and antrum. This study aims to clarify the putative role of PDX1 in gastric carcinogenesis. Methods: PDX1 expression was detected in gastric tissues with chronic gastritis and cancer as well as gastric cancer cell lines by immunohistochemistry, western blot, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR assays. The effects of PDX1 on cell proliferation, apoptosis, clone formation and migration were evaluated using cancer cell lines after transient or stable transfection with PDX1-expressing vector. The ability of PDX1 stable transfectant in tumor formation in xenograft mice was assessed. Results: PDX1 was strongly expressed in normal gastric glands, but was absent in 29 of 39 of human gastric cancer and most gastric cancer cell lines. Negative correlation between PDX1 and Ki-67 expression was found in both gastric tissues and cell lines. Ectopic overexpression of PDX1 significantly inhibited cell proliferation and induced apoptosis, accompanied by the activation of caspases 3, 8, 9 and 10. Overexpression of PDX1 also impaired the ability of cancer cells in clonal formation and migration in vitro. Furthermore, stable transfection with PDX1 reduced the ability of cancer cells in tumor formation in nude mice. Conclusions: PDX1 expression is lost in gastric cancers. Its effect on cell proliferation/apoptosis, migration and tumor formation in vitro and in vivo suggested that this protein functions as a putative tumor suppressor in gastric cancer. © The Author 2008. Published by Oxford University Press. All rights reserved.
 
ISSN0143-3334
2013 Impact Factor: 5.266
 
DOIhttp://dx.doi.org/10.1093/carcin/bgn112
 
ISI Accession Number IDWOS:000258330000005
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorMa, J
 
dc.contributor.authorChen, M
 
dc.contributor.authorWang, J
 
dc.contributor.authorXia, HHX
 
dc.contributor.authorZhu, S
 
dc.contributor.authorLiang, Y
 
dc.contributor.authorGu, Q
 
dc.contributor.authorQiao, L
 
dc.contributor.authorDai, Y
 
dc.contributor.authorZou, B
 
dc.contributor.authorLi, Z
 
dc.contributor.authorZhang, Y
 
dc.contributor.authorLan, H
 
dc.contributor.authorWong, BCY
 
dc.date.accessioned2012-09-05T05:28:35Z
 
dc.date.available2012-09-05T05:28:35Z
 
dc.date.issued2008
 
dc.description.abstractAim: Pancreatic duodenal homeobox-1 (PDX1) is a transcription factor of homeobox genes family important in differentiation and development of the pancreas, duodenum and antrum. This study aims to clarify the putative role of PDX1 in gastric carcinogenesis. Methods: PDX1 expression was detected in gastric tissues with chronic gastritis and cancer as well as gastric cancer cell lines by immunohistochemistry, western blot, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR assays. The effects of PDX1 on cell proliferation, apoptosis, clone formation and migration were evaluated using cancer cell lines after transient or stable transfection with PDX1-expressing vector. The ability of PDX1 stable transfectant in tumor formation in xenograft mice was assessed. Results: PDX1 was strongly expressed in normal gastric glands, but was absent in 29 of 39 of human gastric cancer and most gastric cancer cell lines. Negative correlation between PDX1 and Ki-67 expression was found in both gastric tissues and cell lines. Ectopic overexpression of PDX1 significantly inhibited cell proliferation and induced apoptosis, accompanied by the activation of caspases 3, 8, 9 and 10. Overexpression of PDX1 also impaired the ability of cancer cells in clonal formation and migration in vitro. Furthermore, stable transfection with PDX1 reduced the ability of cancer cells in tumor formation in nude mice. Conclusions: PDX1 expression is lost in gastric cancers. Its effect on cell proliferation/apoptosis, migration and tumor formation in vitro and in vivo suggested that this protein functions as a putative tumor suppressor in gastric cancer. © The Author 2008. Published by Oxford University Press. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationCarcinogenesis, 2008, v. 29 n. 7, p. 1327-1333 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/bgn112
 
dc.identifier.doihttp://dx.doi.org/10.1093/carcin/bgn112
 
dc.identifier.epage1333
 
dc.identifier.hkuros143047
 
dc.identifier.isiWOS:000258330000005
 
dc.identifier.issn0143-3334
2013 Impact Factor: 5.266
 
dc.identifier.issue7
 
dc.identifier.pmid18477649
 
dc.identifier.scopuseid_2-s2.0-49349084817
 
dc.identifier.spage1327
 
dc.identifier.urihttp://hdl.handle.net/10722/163192
 
dc.identifier.volume29
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofCarcinogenesis
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCarcinogenesis. Copyright © Oxford University Press.
 
dc.subject.meshAnimals
 
dc.subject.meshApoptosis - Genetics
 
dc.subject.meshCell Growth Processes - Genetics
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshCell Movement - Genetics
 
dc.subject.meshCell Transformation, Neoplastic - Genetics - Metabolism - Pathology
 
dc.subject.meshDown-Regulation
 
dc.subject.meshFemale
 
dc.subject.meshGene Expression Profiling
 
dc.subject.meshGenes, Tumor Suppressor
 
dc.subject.meshHomeodomain Proteins - Biosynthesis - Genetics
 
dc.subject.meshHumans
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshKi-67 Antigen - Biosynthesis - Genetics
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred Balb C
 
dc.subject.meshMice, Nude
 
dc.subject.meshPrecancerous Conditions - Genetics - Metabolism - Pathology
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshStomach Neoplasms - Genetics - Metabolism - Pathology
 
dc.subject.meshTrans-Activators - Biosynthesis - Genetics
 
dc.subject.meshTransfection
 
dc.titlePancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer
 
dc.typeArticle
 
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<contributor.author>Wang, J</contributor.author>
<contributor.author>Xia, HHX</contributor.author>
<contributor.author>Zhu, S</contributor.author>
<contributor.author>Liang, Y</contributor.author>
<contributor.author>Gu, Q</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University