File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Pancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer

TitlePancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer
Authors
Issue Date2008
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
Citation
Carcinogenesis, 2008, v. 29 n. 7, p. 1327-1333 How to Cite?
AbstractAim: Pancreatic duodenal homeobox-1 (PDX1) is a transcription factor of homeobox genes family important in differentiation and development of the pancreas, duodenum and antrum. This study aims to clarify the putative role of PDX1 in gastric carcinogenesis. Methods: PDX1 expression was detected in gastric tissues with chronic gastritis and cancer as well as gastric cancer cell lines by immunohistochemistry, western blot, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR assays. The effects of PDX1 on cell proliferation, apoptosis, clone formation and migration were evaluated using cancer cell lines after transient or stable transfection with PDX1-expressing vector. The ability of PDX1 stable transfectant in tumor formation in xenograft mice was assessed. Results: PDX1 was strongly expressed in normal gastric glands, but was absent in 29 of 39 of human gastric cancer and most gastric cancer cell lines. Negative correlation between PDX1 and Ki-67 expression was found in both gastric tissues and cell lines. Ectopic overexpression of PDX1 significantly inhibited cell proliferation and induced apoptosis, accompanied by the activation of caspases 3, 8, 9 and 10. Overexpression of PDX1 also impaired the ability of cancer cells in clonal formation and migration in vitro. Furthermore, stable transfection with PDX1 reduced the ability of cancer cells in tumor formation in nude mice. Conclusions: PDX1 expression is lost in gastric cancers. Its effect on cell proliferation/apoptosis, migration and tumor formation in vitro and in vivo suggested that this protein functions as a putative tumor suppressor in gastric cancer. © The Author 2008. Published by Oxford University Press. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/163192
ISSN
2014 Impact Factor: 5.334
2014 SCImago Journal Rankings: 2.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, Jen_US
dc.contributor.authorChen, Men_US
dc.contributor.authorWang, Jen_US
dc.contributor.authorXia, HHXen_US
dc.contributor.authorZhu, Sen_US
dc.contributor.authorLiang, Yen_US
dc.contributor.authorGu, Qen_US
dc.contributor.authorQiao, Len_US
dc.contributor.authorDai, Yen_US
dc.contributor.authorZou, Ben_US
dc.contributor.authorLi, Zen_US
dc.contributor.authorZhang, Yen_US
dc.contributor.authorLan, Hen_US
dc.contributor.authorWong, BCYen_US
dc.date.accessioned2012-09-05T05:28:35Z-
dc.date.available2012-09-05T05:28:35Z-
dc.date.issued2008en_US
dc.identifier.citationCarcinogenesis, 2008, v. 29 n. 7, p. 1327-1333en_US
dc.identifier.issn0143-3334en_US
dc.identifier.urihttp://hdl.handle.net/10722/163192-
dc.description.abstractAim: Pancreatic duodenal homeobox-1 (PDX1) is a transcription factor of homeobox genes family important in differentiation and development of the pancreas, duodenum and antrum. This study aims to clarify the putative role of PDX1 in gastric carcinogenesis. Methods: PDX1 expression was detected in gastric tissues with chronic gastritis and cancer as well as gastric cancer cell lines by immunohistochemistry, western blot, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR assays. The effects of PDX1 on cell proliferation, apoptosis, clone formation and migration were evaluated using cancer cell lines after transient or stable transfection with PDX1-expressing vector. The ability of PDX1 stable transfectant in tumor formation in xenograft mice was assessed. Results: PDX1 was strongly expressed in normal gastric glands, but was absent in 29 of 39 of human gastric cancer and most gastric cancer cell lines. Negative correlation between PDX1 and Ki-67 expression was found in both gastric tissues and cell lines. Ectopic overexpression of PDX1 significantly inhibited cell proliferation and induced apoptosis, accompanied by the activation of caspases 3, 8, 9 and 10. Overexpression of PDX1 also impaired the ability of cancer cells in clonal formation and migration in vitro. Furthermore, stable transfection with PDX1 reduced the ability of cancer cells in tumor formation in nude mice. Conclusions: PDX1 expression is lost in gastric cancers. Its effect on cell proliferation/apoptosis, migration and tumor formation in vitro and in vivo suggested that this protein functions as a putative tumor suppressor in gastric cancer. © The Author 2008. Published by Oxford University Press. All rights reserved.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/en_US
dc.relation.ispartofCarcinogenesisen_US
dc.rightsCarcinogenesis. Copyright © Oxford University Press.-
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Geneticsen_US
dc.subject.meshCell Growth Processes - Geneticsen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Movement - Geneticsen_US
dc.subject.meshCell Transformation, Neoplastic - Genetics - Metabolism - Pathologyen_US
dc.subject.meshDown-Regulationen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGenes, Tumor Suppressoren_US
dc.subject.meshHomeodomain Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshKi-67 Antigen - Biosynthesis - Geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshPrecancerous Conditions - Genetics - Metabolism - Pathologyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshStomach Neoplasms - Genetics - Metabolism - Pathologyen_US
dc.subject.meshTrans-Activators - Biosynthesis - Geneticsen_US
dc.subject.meshTransfectionen_US
dc.titlePancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric canceren_US
dc.typeArticleen_US
dc.identifier.emailWang, J:jidewang@gmail.comen_US
dc.identifier.emailQiao, L:lq8688@hotmail.comen_US
dc.identifier.emailWong, BCY:bcywong@hku.hken_US
dc.identifier.authorityWang, J=rp00491en_US
dc.identifier.authorityQiao, L=rp00513en_US
dc.identifier.authorityWong, BCY=rp00429en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/carcin/bgn112en_US
dc.identifier.pmid18477649en_US
dc.identifier.scopuseid_2-s2.0-49349084817en_US
dc.identifier.hkuros143047-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49349084817&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume29en_US
dc.identifier.issue7en_US
dc.identifier.spage1327en_US
dc.identifier.epage1333en_US
dc.identifier.isiWOS:000258330000005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridMa, J=35275386200en_US
dc.identifier.scopusauthoridChen, M=8642044500en_US
dc.identifier.scopusauthoridWang, J=35309087500en_US
dc.identifier.scopusauthoridXia, HHX=8757161400en_US
dc.identifier.scopusauthoridZhu, S=7404391208en_US
dc.identifier.scopusauthoridLiang, Y=12804573800en_US
dc.identifier.scopusauthoridGu, Q=24469982400en_US
dc.identifier.scopusauthoridQiao, L=7202151719en_US
dc.identifier.scopusauthoridDai, Y=7401512993en_US
dc.identifier.scopusauthoridZou, B=35228257300en_US
dc.identifier.scopusauthoridLi, Z=24171072000en_US
dc.identifier.scopusauthoridZhang, Y=36129128700en_US
dc.identifier.scopusauthoridLan, H=24544799000en_US
dc.identifier.scopusauthoridWong, BCY=7402023340en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats