Article: Pancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer

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TitlePancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer
AuthorsMa, J1 2
Chen, M2
Wang, J1
Xia, HHX1
Zhu, S2
Liang, Y2
Gu, Q1
Qiao, L1
Dai, Y1
Zou, B1
Li, Z1
Zhang, Y1
Lan, H1
Wong, BCY1
Issue Date2008
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
CitationCarcinogenesis, 2008, v. 29 n. 7, p. 1327-1333 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/bgn112
AbstractAim: Pancreatic duodenal homeobox-1 (PDX1) is a transcription factor of homeobox genes family important in differentiation and development of the pancreas, duodenum and antrum. This study aims to clarify the putative role of PDX1 in gastric carcinogenesis. Methods: PDX1 expression was detected in gastric tissues with chronic gastritis and cancer as well as gastric cancer cell lines by immunohistochemistry, western blot, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR assays. The effects of PDX1 on cell proliferation, apoptosis, clone formation and migration were evaluated using cancer cell lines after transient or stable transfection with PDX1-expressing vector. The ability of PDX1 stable transfectant in tumor formation in xenograft mice was assessed. Results: PDX1 was strongly expressed in normal gastric glands, but was absent in 29 of 39 of human gastric cancer and most gastric cancer cell lines. Negative correlation between PDX1 and Ki-67 expression was found in both gastric tissues and cell lines. Ectopic overexpression of PDX1 significantly inhibited cell proliferation and induced apoptosis, accompanied by the activation of caspases 3, 8, 9 and 10. Overexpression of PDX1 also impaired the ability of cancer cells in clonal formation and migration in vitro. Furthermore, stable transfection with PDX1 reduced the ability of cancer cells in tumor formation in nude mice. Conclusions: PDX1 expression is lost in gastric cancers. Its effect on cell proliferation/apoptosis, migration and tumor formation in vitro and in vivo suggested that this protein functions as a putative tumor suppressor in gastric cancer. © The Author 2008. Published by Oxford University Press. All rights reserved.
ISSN0143-3334
2011 Impact Factor: 5.702
2011 SCImago Journal Rankings: 0.692
DOIhttp://dx.doi.org/10.1093/carcin/bgn112
ISI Accession Number IDWOS:000258330000005
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorMa, J
dc.contributor.authorChen, M
dc.contributor.authorWang, J
dc.contributor.authorXia, HHX
dc.contributor.authorZhu, S
dc.contributor.authorLiang, Y
dc.contributor.authorGu, Q
dc.contributor.authorQiao, L
dc.contributor.authorDai, Y
dc.contributor.authorZou, B
dc.contributor.authorLi, Z
dc.contributor.authorZhang, Y
dc.contributor.authorLan, H
dc.contributor.authorWong, BCY
dc.date.accessioned2012-09-05T05:28:35Z
dc.date.available2012-09-05T05:28:35Z
dc.date.issued2008
dc.description.abstractAim: Pancreatic duodenal homeobox-1 (PDX1) is a transcription factor of homeobox genes family important in differentiation and development of the pancreas, duodenum and antrum. This study aims to clarify the putative role of PDX1 in gastric carcinogenesis. Methods: PDX1 expression was detected in gastric tissues with chronic gastritis and cancer as well as gastric cancer cell lines by immunohistochemistry, western blot, reverse transcription-polymerase chain reaction (RT-PCR) or quantitative real-time RT-PCR assays. The effects of PDX1 on cell proliferation, apoptosis, clone formation and migration were evaluated using cancer cell lines after transient or stable transfection with PDX1-expressing vector. The ability of PDX1 stable transfectant in tumor formation in xenograft mice was assessed. Results: PDX1 was strongly expressed in normal gastric glands, but was absent in 29 of 39 of human gastric cancer and most gastric cancer cell lines. Negative correlation between PDX1 and Ki-67 expression was found in both gastric tissues and cell lines. Ectopic overexpression of PDX1 significantly inhibited cell proliferation and induced apoptosis, accompanied by the activation of caspases 3, 8, 9 and 10. Overexpression of PDX1 also impaired the ability of cancer cells in clonal formation and migration in vitro. Furthermore, stable transfection with PDX1 reduced the ability of cancer cells in tumor formation in nude mice. Conclusions: PDX1 expression is lost in gastric cancers. Its effect on cell proliferation/apoptosis, migration and tumor formation in vitro and in vivo suggested that this protein functions as a putative tumor suppressor in gastric cancer. © The Author 2008. Published by Oxford University Press. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCarcinogenesis, 2008, v. 29 n. 7, p. 1327-1333 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/bgn112
dc.identifier.doihttp://dx.doi.org/10.1093/carcin/bgn112
dc.identifier.epage1333
dc.identifier.hkuros143047
dc.identifier.isiWOS:000258330000005
dc.identifier.issn0143-3334
2011 Impact Factor: 5.702
2011 SCImago Journal Rankings: 0.692
dc.identifier.issue7
dc.identifier.pmid18477649
dc.identifier.scopuseid_2-s2.0-49349084817
dc.identifier.spage1327
dc.identifier.urihttp://hdl.handle.net/10722/163192
dc.identifier.volume29
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofCarcinogenesis
dc.relation.referencesReferences in Scopus
dc.rightsCarcinogenesis. Copyright © Oxford University Press.
dc.subject.meshAnimals
dc.subject.meshApoptosis - Genetics
dc.subject.meshCell Growth Processes - Genetics
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Movement - Genetics
dc.subject.meshCell Transformation, Neoplastic - Genetics - Metabolism - Pathology
dc.subject.meshDown-Regulation
dc.subject.meshFemale
dc.subject.meshGene Expression Profiling
dc.subject.meshGenes, Tumor Suppressor
dc.subject.meshHomeodomain Proteins - Biosynthesis - Genetics
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshKi-67 Antigen - Biosynthesis - Genetics
dc.subject.meshMice
dc.subject.meshMice, Inbred Balb C
dc.subject.meshMice, Nude
dc.subject.meshPrecancerous Conditions - Genetics - Metabolism - Pathology
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshStomach Neoplasms - Genetics - Metabolism - Pathology
dc.subject.meshTrans-Activators - Biosynthesis - Genetics
dc.subject.meshTransfection
dc.titlePancreatic duodenal homeobox-1 (PDX1) functions as a tumor suppressor in gastric cancer
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University