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Article: Systemic lupus erythematosus patients have increased number of circulating plasmacytoid dendritic cells, but decreased myeloid dendritic cells with deficient CD83 expression
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TitleSystemic lupus erythematosus patients have increased number of circulating plasmacytoid dendritic cells, but decreased myeloid dendritic cells with deficient CD83 expression
 
AuthorsJin, O2
Kavikondala, S2
Sun, L3
Fu, R2
Mok, MY2
Chan, A2
Yeung, J2
Lau, CS2 1
 
Issue Date2008
 
PublisherSage Publications Ltd. The Journal's web site is located at http://lup.sagepub.com
 
CitationLupus, 2008, v. 17 n. 7, p. 654-662 [How to Cite?]
DOI: http://dx.doi.org/10.1177/0961203308089410
 
AbstractDendritic cells (DCs) are functionally abnormal in systemic lupus erythematosus (SLE). However, previous studies have involved in-vitro cytokine-induced DCs. In this investigation, directly isolated circulating plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) in SLE were studied. Blood dendritic cell antigen (BDCA)-4 and BDCA-1 magnetic isolation kits were used to isolate blood pDCs and mDCs from 30 SLE patients and 36 controls. Their number and surface markers, and their relationship with lupus disease activity were evaluated. The percentage of pDCs per peripheral blood mononuclear cells was higher in SLE (0.33 ± 0.14) than in controls (0.16 ± 0.09, P < 0.01), but that of mDCs was lower in SLE (0.43 ± 0.14) than in controls (0.63 ± 0.32; P < 0.01). In controls, both pDCs and mDCs expressed high levels of MHC-II, however, the expression of CD86, CD83 and CCR7 on pDCs were significantly lower than that on mDCs (all P < 0.05). mDCs from patients with SLE, particularly those with active disease, expressed lower CD83 than controls. In health, circulating mDCs may be more efficient than pDCs in stimulating T cells. In SLE, the increased number of circulating pDCs supports a pathogenic role for these cells, and the decreased mDC number and CD83 expression may explain the susceptibility to infections in these patients. © 2008 SAGE Publications.
 
ISSN0961-2033
2012 Impact Factor: 2.783
2012 SCImago Journal Rankings: 0.985
 
DOIhttp://dx.doi.org/10.1177/0961203308089410
 
ISI Accession Number IDWOS:000257934200008
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorJin, O
 
dc.contributor.authorKavikondala, S
 
dc.contributor.authorSun, L
 
dc.contributor.authorFu, R
 
dc.contributor.authorMok, MY
 
dc.contributor.authorChan, A
 
dc.contributor.authorYeung, J
 
dc.contributor.authorLau, CS
 
dc.date.accessioned2012-09-05T05:28:33Z
 
dc.date.available2012-09-05T05:28:33Z
 
dc.date.issued2008
 
dc.description.abstractDendritic cells (DCs) are functionally abnormal in systemic lupus erythematosus (SLE). However, previous studies have involved in-vitro cytokine-induced DCs. In this investigation, directly isolated circulating plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) in SLE were studied. Blood dendritic cell antigen (BDCA)-4 and BDCA-1 magnetic isolation kits were used to isolate blood pDCs and mDCs from 30 SLE patients and 36 controls. Their number and surface markers, and their relationship with lupus disease activity were evaluated. The percentage of pDCs per peripheral blood mononuclear cells was higher in SLE (0.33 ± 0.14) than in controls (0.16 ± 0.09, P < 0.01), but that of mDCs was lower in SLE (0.43 ± 0.14) than in controls (0.63 ± 0.32; P < 0.01). In controls, both pDCs and mDCs expressed high levels of MHC-II, however, the expression of CD86, CD83 and CCR7 on pDCs were significantly lower than that on mDCs (all P < 0.05). mDCs from patients with SLE, particularly those with active disease, expressed lower CD83 than controls. In health, circulating mDCs may be more efficient than pDCs in stimulating T cells. In SLE, the increased number of circulating pDCs supports a pathogenic role for these cells, and the decreased mDC number and CD83 expression may explain the susceptibility to infections in these patients. © 2008 SAGE Publications.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationLupus, 2008, v. 17 n. 7, p. 654-662 [How to Cite?]
DOI: http://dx.doi.org/10.1177/0961203308089410
 
dc.identifier.citeulike8333075
 
dc.identifier.doihttp://dx.doi.org/10.1177/0961203308089410
 
dc.identifier.epage662
 
dc.identifier.hkuros148449
 
dc.identifier.isiWOS:000257934200008
 
dc.identifier.issn0961-2033
2012 Impact Factor: 2.783
2012 SCImago Journal Rankings: 0.985
 
dc.identifier.issue7
 
dc.identifier.pmid18625638
 
dc.identifier.scopuseid_2-s2.0-48249107477
 
dc.identifier.spage654
 
dc.identifier.urihttp://hdl.handle.net/10722/163188
 
dc.identifier.volume17
 
dc.languageeng
 
dc.publisherSage Publications Ltd. The Journal's web site is located at http://lup.sagepub.com
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofLupus
 
dc.relation.referencesReferences in Scopus
 
dc.rightsLupus. Copyright © Sage Publications Ltd.
 
dc.subject.meshAdult
 
dc.subject.meshAntigens, Cd - Immunology
 
dc.subject.meshBiological Markers - Metabolism
 
dc.subject.meshCell Separation
 
dc.subject.meshCell Shape
 
dc.subject.meshDendritic Cells - Cytology - Immunology
 
dc.subject.meshFemale
 
dc.subject.meshHumans
 
dc.subject.meshImmunoglobulins - Immunology
 
dc.subject.meshImmunomagnetic Separation
 
dc.subject.meshLupus Erythematosus, Systemic - Blood - Immunology - Pathology
 
dc.subject.meshMale
 
dc.subject.meshMembrane Glycoproteins - Immunology
 
dc.subject.meshMiddle Aged
 
dc.subject.meshMyeloid Cells - Cytology - Immunology
 
dc.titleSystemic lupus erythematosus patients have increased number of circulating plasmacytoid dendritic cells, but decreased myeloid dendritic cells with deficient CD83 expression
 
dc.typeArticle
 
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<contributor.author>Mok, MY</contributor.author>
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<description.abstract>Dendritic cells (DCs) are functionally abnormal in systemic lupus erythematosus (SLE). However, previous studies have involved in-vitro cytokine-induced DCs. In this investigation, directly isolated circulating plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) in SLE were studied. Blood dendritic cell antigen (BDCA)-4 and BDCA-1 magnetic isolation kits were used to isolate blood pDCs and mDCs from 30 SLE patients and 36 controls. Their number and surface markers, and their relationship with lupus disease activity were evaluated. The percentage of pDCs per peripheral blood mononuclear cells was higher in SLE (0.33 &#177; 0.14) than in controls (0.16 &#177; 0.09, P &lt; 0.01), but that of mDCs was lower in SLE (0.43 &#177; 0.14) than in controls (0.63 &#177; 0.32; P &lt; 0.01). In controls, both pDCs and mDCs expressed high levels of MHC-II, however, the expression of CD86, CD83 and CCR7 on pDCs were significantly lower than that on mDCs (all P &lt; 0.05). mDCs from patients with SLE, particularly those with active disease, expressed lower CD83 than controls. In health, circulating mDCs may be more efficient than pDCs in stimulating T cells. In SLE, the increased number of circulating pDCs supports a pathogenic role for these cells, and the decreased mDC number and CD83 expression may explain the susceptibility to infections in these patients. &#169; 2008 SAGE Publications.</description.abstract>
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Author Affiliations
  1. University of Dundee College of Medicine, Dentistry and Nursing
  2. The University of Hong Kong
  3. The Affiliated DrumTower Hospital of Nanjing University Medical School