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Article: Systemic lupus erythematosus patients have increased number of circulating plasmacytoid dendritic cells, but decreased myeloid dendritic cells with deficient CD83 expression

TitleSystemic lupus erythematosus patients have increased number of circulating plasmacytoid dendritic cells, but decreased myeloid dendritic cells with deficient CD83 expression
Authors
Issue Date2008
PublisherSage Publications Ltd. The Journal's web site is located at http://lup.sagepub.com
Citation
Lupus, 2008, v. 17 n. 7, p. 654-662 How to Cite?
AbstractDendritic cells (DCs) are functionally abnormal in systemic lupus erythematosus (SLE). However, previous studies have involved in-vitro cytokine-induced DCs. In this investigation, directly isolated circulating plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) in SLE were studied. Blood dendritic cell antigen (BDCA)-4 and BDCA-1 magnetic isolation kits were used to isolate blood pDCs and mDCs from 30 SLE patients and 36 controls. Their number and surface markers, and their relationship with lupus disease activity were evaluated. The percentage of pDCs per peripheral blood mononuclear cells was higher in SLE (0.33 ± 0.14) than in controls (0.16 ± 0.09, P < 0.01), but that of mDCs was lower in SLE (0.43 ± 0.14) than in controls (0.63 ± 0.32; P < 0.01). In controls, both pDCs and mDCs expressed high levels of MHC-II, however, the expression of CD86, CD83 and CCR7 on pDCs were significantly lower than that on mDCs (all P < 0.05). mDCs from patients with SLE, particularly those with active disease, expressed lower CD83 than controls. In health, circulating mDCs may be more efficient than pDCs in stimulating T cells. In SLE, the increased number of circulating pDCs supports a pathogenic role for these cells, and the decreased mDC number and CD83 expression may explain the susceptibility to infections in these patients. © 2008 SAGE Publications.
Persistent Identifierhttp://hdl.handle.net/10722/163188
ISSN
2014 Impact Factor: 2.197
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Oen_US
dc.contributor.authorKavikondala, Sen_US
dc.contributor.authorSun, Len_US
dc.contributor.authorFu, Ren_US
dc.contributor.authorMok, MYen_US
dc.contributor.authorChan, Aen_US
dc.contributor.authorYeung, Jen_US
dc.contributor.authorLau, CSen_US
dc.date.accessioned2012-09-05T05:28:33Z-
dc.date.available2012-09-05T05:28:33Z-
dc.date.issued2008en_US
dc.identifier.citationLupus, 2008, v. 17 n. 7, p. 654-662en_US
dc.identifier.issn0961-2033en_US
dc.identifier.urihttp://hdl.handle.net/10722/163188-
dc.description.abstractDendritic cells (DCs) are functionally abnormal in systemic lupus erythematosus (SLE). However, previous studies have involved in-vitro cytokine-induced DCs. In this investigation, directly isolated circulating plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) in SLE were studied. Blood dendritic cell antigen (BDCA)-4 and BDCA-1 magnetic isolation kits were used to isolate blood pDCs and mDCs from 30 SLE patients and 36 controls. Their number and surface markers, and their relationship with lupus disease activity were evaluated. The percentage of pDCs per peripheral blood mononuclear cells was higher in SLE (0.33 ± 0.14) than in controls (0.16 ± 0.09, P < 0.01), but that of mDCs was lower in SLE (0.43 ± 0.14) than in controls (0.63 ± 0.32; P < 0.01). In controls, both pDCs and mDCs expressed high levels of MHC-II, however, the expression of CD86, CD83 and CCR7 on pDCs were significantly lower than that on mDCs (all P < 0.05). mDCs from patients with SLE, particularly those with active disease, expressed lower CD83 than controls. In health, circulating mDCs may be more efficient than pDCs in stimulating T cells. In SLE, the increased number of circulating pDCs supports a pathogenic role for these cells, and the decreased mDC number and CD83 expression may explain the susceptibility to infections in these patients. © 2008 SAGE Publications.en_US
dc.languageengen_US
dc.publisherSage Publications Ltd. The Journal's web site is located at http://lup.sagepub.comen_US
dc.relation.ispartofLupusen_US
dc.rightsLupus. Copyright © Sage Publications Ltd.-
dc.subject.meshAdulten_US
dc.subject.meshAntigens, Cd - Immunologyen_US
dc.subject.meshBiological Markers - Metabolismen_US
dc.subject.meshCell Separationen_US
dc.subject.meshCell Shapeen_US
dc.subject.meshDendritic Cells - Cytology - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulins - Immunologyen_US
dc.subject.meshImmunomagnetic Separationen_US
dc.subject.meshLupus Erythematosus, Systemic - Blood - Immunology - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Glycoproteins - Immunologyen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMyeloid Cells - Cytology - Immunologyen_US
dc.titleSystemic lupus erythematosus patients have increased number of circulating plasmacytoid dendritic cells, but decreased myeloid dendritic cells with deficient CD83 expressionen_US
dc.typeArticleen_US
dc.identifier.emailMok, MY:temy@hkucc.hku.hken_US
dc.identifier.emailLau, CS:cslau@hku.hken_US
dc.identifier.authorityMok, MY=rp00490en_US
dc.identifier.authorityLau, CS=rp01348en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1177/0961203308089410en_US
dc.identifier.pmid18625638en_US
dc.identifier.scopuseid_2-s2.0-48249107477en_US
dc.identifier.hkuros148449-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-48249107477&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume17en_US
dc.identifier.issue7en_US
dc.identifier.spage654en_US
dc.identifier.epage662en_US
dc.identifier.isiWOS:000257934200008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridJin, O=7004432895en_US
dc.identifier.scopusauthoridKavikondala, S=14819602600en_US
dc.identifier.scopusauthoridSun, L=35265069800en_US
dc.identifier.scopusauthoridFu, R=36782017400en_US
dc.identifier.scopusauthoridMok, MY=7006024184en_US
dc.identifier.scopusauthoridChan, A=7403167812en_US
dc.identifier.scopusauthoridYeung, J=24482785000en_US
dc.identifier.scopusauthoridLau, CS=14035682100en_US
dc.identifier.citeulike8333075-

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