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Article: XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells

TitleXIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells
Authors
KeywordsAngiogenesis
Apoptosis
Endothelial cells
XAF1
Issue Date2008
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBI
Citation
Tumor Biology, 2008, v. 29 n. 2, p. 122-129 How to Cite?
AbstractObjectives: XIAP-associated factor 1 (XAF1) is a tumor suppressor gene, but its role in angiogenesis is unknown. We investigated whether XAF1 has any antiangiogenesis effect. Methods: MS1 (a mouse endothelial cell line) was infected with an adenoviral vector ZD55-XAF1. Controls were uninfected or infected with ZD55-EGFP. Wound healing assay and tube formation assay were used to assess angiogenesis. Cell proliferation was detected by WST-1 assay, and apoptosis was detected by TUNEL and APOPercentage™ assays. Results: ZD55-XAF1 significantly upregulated XAF1, which was associated with decreased cell proliferation, migration and tube formation of MS1 cells. Ectopic overexpression of XAF1 induced apoptosis in MS1 and also sensitized cells to 5-fluorouracil-induced apoptosis. A significant decrease in the expression of Tie-1, Ang-1, Ang-2 and c-Myc was observed. Conclusions: Our data suggest that XAF1 possesses a potential antiangiogenesis effect. Suppressed expression of Tie-1, Ang-1, Ang-2 and c-Myc may be mechanistically responsible for the observed antiangiogenesis effect. Copyright © 2008 S. Karger AG.
Persistent Identifierhttp://hdl.handle.net/10722/163186
ISSN
2015 Impact Factor: 2.926
2015 SCImago Journal Rankings: 0.989
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQiao, Len_HK
dc.contributor.authorGu, Qen_HK
dc.contributor.authorDai, Yen_HK
dc.contributor.authorShen, Zen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorQi, Ren_HK
dc.contributor.authorMa, Jen_HK
dc.contributor.authorZou, Ben_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorLan, HYen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2012-09-05T05:28:31Z-
dc.date.available2012-09-05T05:28:31Z-
dc.date.issued2008en_HK
dc.identifier.citationTumor Biology, 2008, v. 29 n. 2, p. 122-129en_HK
dc.identifier.issn1010-4283en_HK
dc.identifier.urihttp://hdl.handle.net/10722/163186-
dc.description.abstractObjectives: XIAP-associated factor 1 (XAF1) is a tumor suppressor gene, but its role in angiogenesis is unknown. We investigated whether XAF1 has any antiangiogenesis effect. Methods: MS1 (a mouse endothelial cell line) was infected with an adenoviral vector ZD55-XAF1. Controls were uninfected or infected with ZD55-EGFP. Wound healing assay and tube formation assay were used to assess angiogenesis. Cell proliferation was detected by WST-1 assay, and apoptosis was detected by TUNEL and APOPercentage™ assays. Results: ZD55-XAF1 significantly upregulated XAF1, which was associated with decreased cell proliferation, migration and tube formation of MS1 cells. Ectopic overexpression of XAF1 induced apoptosis in MS1 and also sensitized cells to 5-fluorouracil-induced apoptosis. A significant decrease in the expression of Tie-1, Ang-1, Ang-2 and c-Myc was observed. Conclusions: Our data suggest that XAF1 possesses a potential antiangiogenesis effect. Suppressed expression of Tie-1, Ang-1, Ang-2 and c-Myc may be mechanistically responsible for the observed antiangiogenesis effect. Copyright © 2008 S. Karger AG.en_HK
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBIen_HK
dc.relation.ispartofTumor Biologyen_HK
dc.subjectAngiogenesisen_HK
dc.subjectApoptosisen_HK
dc.subjectEndothelial cellsen_HK
dc.subjectXAF1en_HK
dc.subject.meshAdenoviridae - Geneticsen_US
dc.subject.meshAngiotensin I - Metabolismen_US
dc.subject.meshAngiotensin Ii - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Physiologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshEndothelium, Vascular - Cytology - Metabolismen_US
dc.subject.meshF-Box Proteins - Genetics - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshNeovascularization, Physiologic - Physiologyen_US
dc.subject.meshProto-Oncogene Proteins C-Myc - Metabolismen_US
dc.subject.meshReceptor, Tie-1 - Metabolismen_US
dc.subject.meshSignal Transduction - Physiologyen_US
dc.subject.meshUp-Regulation - Physiologyen_US
dc.titleXIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailQiao, L: lq8688@hotmail.comen_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityQiao, L=rp00513en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000137831en_HK
dc.identifier.pmid18544999-
dc.identifier.scopuseid_2-s2.0-47949129803en_HK
dc.identifier.hkuros141379-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47949129803&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue2en_HK
dc.identifier.spage122en_HK
dc.identifier.epage129en_HK
dc.identifier.isiWOS:000257903500007-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridQiao, L=7202151719en_HK
dc.identifier.scopusauthoridGu, Q=24469982400en_HK
dc.identifier.scopusauthoridDai, Y=7401512993en_HK
dc.identifier.scopusauthoridShen, Z=35759640300en_HK
dc.identifier.scopusauthoridLiu, X=7409290398en_HK
dc.identifier.scopusauthoridQi, R=55111639100en_HK
dc.identifier.scopusauthoridMa, J=35275386200en_HK
dc.identifier.scopusauthoridZou, B=35228257300en_HK
dc.identifier.scopusauthoridLi, Z=24171072000en_HK
dc.identifier.scopusauthoridLan, HY=7102710832en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK

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