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- Publisher Website: 10.1159/000137831
- Scopus: eid_2-s2.0-47949129803
- PMID: 18544999
- WOS: WOS:000257903500007
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Article: XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells
Title | XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells |
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Authors | |
Keywords | Angiogenesis Apoptosis Endothelial cells XAF1 |
Issue Date | 2008 |
Publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/TBI |
Citation | Tumor Biology, 2008, v. 29 n. 2, p. 122-129 How to Cite? |
Abstract | Objectives: XIAP-associated factor 1 (XAF1) is a tumor suppressor gene, but its role in angiogenesis is unknown. We investigated whether XAF1 has any antiangiogenesis effect. Methods: MS1 (a mouse endothelial cell line) was infected with an adenoviral vector ZD55-XAF1. Controls were uninfected or infected with ZD55-EGFP. Wound healing assay and tube formation assay were used to assess angiogenesis. Cell proliferation was detected by WST-1 assay, and apoptosis was detected by TUNEL and APOPercentage™ assays. Results: ZD55-XAF1 significantly upregulated XAF1, which was associated with decreased cell proliferation, migration and tube formation of MS1 cells. Ectopic overexpression of XAF1 induced apoptosis in MS1 and also sensitized cells to 5-fluorouracil-induced apoptosis. A significant decrease in the expression of Tie-1, Ang-1, Ang-2 and c-Myc was observed. Conclusions: Our data suggest that XAF1 possesses a potential antiangiogenesis effect. Suppressed expression of Tie-1, Ang-1, Ang-2 and c-Myc may be mechanistically responsible for the observed antiangiogenesis effect. Copyright © 2008 S. Karger AG. |
Persistent Identifier | http://hdl.handle.net/10722/163186 |
ISSN | 2016 Impact Factor: 3.650 2023 SCImago Journal Rankings: 0.576 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Qiao, L | en_HK |
dc.contributor.author | Gu, Q | en_HK |
dc.contributor.author | Dai, Y | en_HK |
dc.contributor.author | Shen, Z | en_HK |
dc.contributor.author | Liu, X | en_HK |
dc.contributor.author | Qi, R | en_HK |
dc.contributor.author | Ma, J | en_HK |
dc.contributor.author | Zou, B | en_HK |
dc.contributor.author | Li, Z | en_HK |
dc.contributor.author | Lan, HY | en_HK |
dc.contributor.author | Wong, BCY | en_HK |
dc.date.accessioned | 2012-09-05T05:28:31Z | - |
dc.date.available | 2012-09-05T05:28:31Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Tumor Biology, 2008, v. 29 n. 2, p. 122-129 | en_HK |
dc.identifier.issn | 1010-4283 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/163186 | - |
dc.description.abstract | Objectives: XIAP-associated factor 1 (XAF1) is a tumor suppressor gene, but its role in angiogenesis is unknown. We investigated whether XAF1 has any antiangiogenesis effect. Methods: MS1 (a mouse endothelial cell line) was infected with an adenoviral vector ZD55-XAF1. Controls were uninfected or infected with ZD55-EGFP. Wound healing assay and tube formation assay were used to assess angiogenesis. Cell proliferation was detected by WST-1 assay, and apoptosis was detected by TUNEL and APOPercentage™ assays. Results: ZD55-XAF1 significantly upregulated XAF1, which was associated with decreased cell proliferation, migration and tube formation of MS1 cells. Ectopic overexpression of XAF1 induced apoptosis in MS1 and also sensitized cells to 5-fluorouracil-induced apoptosis. A significant decrease in the expression of Tie-1, Ang-1, Ang-2 and c-Myc was observed. Conclusions: Our data suggest that XAF1 possesses a potential antiangiogenesis effect. Suppressed expression of Tie-1, Ang-1, Ang-2 and c-Myc may be mechanistically responsible for the observed antiangiogenesis effect. Copyright © 2008 S. Karger AG. | en_HK |
dc.language | eng | en_US |
dc.publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/TBI | en_HK |
dc.relation.ispartof | Tumor Biology | en_HK |
dc.subject | Angiogenesis | en_HK |
dc.subject | Apoptosis | en_HK |
dc.subject | Endothelial cells | en_HK |
dc.subject | XAF1 | en_HK |
dc.subject.mesh | Adenoviridae - Genetics | en_US |
dc.subject.mesh | Angiotensin I - Metabolism | en_US |
dc.subject.mesh | Angiotensin Ii - Metabolism | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Apoptosis - Physiology | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Cell Proliferation | en_US |
dc.subject.mesh | Endothelium, Vascular - Cytology - Metabolism | en_US |
dc.subject.mesh | F-Box Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Neovascularization, Physiologic - Physiology | en_US |
dc.subject.mesh | Proto-Oncogene Proteins C-Myc - Metabolism | en_US |
dc.subject.mesh | Receptor, Tie-1 - Metabolism | en_US |
dc.subject.mesh | Signal Transduction - Physiology | en_US |
dc.subject.mesh | Up-Regulation - Physiology | en_US |
dc.title | XIAP-associated factor 1 (XAF1) suppresses angiogenesis in mouse endothelial cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Qiao, L: lq8688@hotmail.com | en_HK |
dc.identifier.email | Wong, BCY: bcywong@hku.hk | en_HK |
dc.identifier.authority | Qiao, L=rp00513 | en_HK |
dc.identifier.authority | Wong, BCY=rp00429 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1159/000137831 | en_HK |
dc.identifier.pmid | 18544999 | - |
dc.identifier.scopus | eid_2-s2.0-47949129803 | en_HK |
dc.identifier.hkuros | 141379 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-47949129803&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 29 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 122 | en_HK |
dc.identifier.epage | 129 | en_HK |
dc.identifier.isi | WOS:000257903500007 | - |
dc.publisher.place | Switzerland | en_HK |
dc.identifier.scopusauthorid | Qiao, L=7202151719 | en_HK |
dc.identifier.scopusauthorid | Gu, Q=24469982400 | en_HK |
dc.identifier.scopusauthorid | Dai, Y=7401512993 | en_HK |
dc.identifier.scopusauthorid | Shen, Z=35759640300 | en_HK |
dc.identifier.scopusauthorid | Liu, X=7409290398 | en_HK |
dc.identifier.scopusauthorid | Qi, R=55111639100 | en_HK |
dc.identifier.scopusauthorid | Ma, J=35275386200 | en_HK |
dc.identifier.scopusauthorid | Zou, B=35228257300 | en_HK |
dc.identifier.scopusauthorid | Li, Z=24171072000 | en_HK |
dc.identifier.scopusauthorid | Lan, HY=7102710832 | en_HK |
dc.identifier.scopusauthorid | Wong, BCY=7402023340 | en_HK |
dc.identifier.issnl | 1010-4283 | - |