File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Ultrasound-contrast agent mediated naked gene delivery in the peritoneal cavity of adult rat

TitleUltrasound-contrast agent mediated naked gene delivery in the peritoneal cavity of adult rat
Authors
Issue Date2007
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gt
Citation
Gene Therapy, 2007, v. 14 n. 24, p. 1712-1720 How to Cite?
AbstractGene transfer into the peritoneal cavity by nonviral methods may provide an effective therapeutic approach for peritoneal diseases. Herein, we investigated the feasibility and the effectiveness of ultrasound-microbubble-mediated delivery of naked plasmid DNA into the peritoneal cavity in rats. Following the intraperitoneal or the intravenous administration of a mixture of plasmid DNA (100 μg) and ultrasound contrast agent microbubbles, an ultrasound transducer was applied on the abdominal wall. The reporter pTRE plasmid encoding Smad7 was used to evaluate transfection efficiency. Smad7 expression was induced by doxycycline in drinking water. We detected less than 10% apoptotic cells and no inflammatory reaction in peritoneal tissues following the ultrasound-microbubble-mediated transfection. More importantly, the insonation significantly improved the transfection efficiency in peritoneal tissues. The transfection efficiency by intraperitoneal delivery route was higher than the intravenous route. The reporter gene, pTRE-Smad7, was readily detected in the parietal peritoneum, mesentery, greater omentum and adipose tissue. The peak of transgene expression occurred 2 days after transfection and the transgene expression diminished in a time-dependent manner thereafter. Overall, the effectiveness and simplicity of the ultrasound-microbubble-mediated system may provide a promising nonviral means for improving gene delivery for treating peritoneal diseases in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/163132
ISSN
2015 Impact Factor: 3.242
2015 SCImago Journal Rankings: 1.519
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, Hen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, LYYen_HK
dc.contributor.authorTsang, AWLen_HK
dc.contributor.authorLam, MFen_HK
dc.contributor.authorLan, HYen_HK
dc.contributor.authorLai, KNen_HK
dc.date.accessioned2012-09-05T05:28:00Z-
dc.date.available2012-09-05T05:28:00Z-
dc.date.issued2007en_HK
dc.identifier.citationGene Therapy, 2007, v. 14 n. 24, p. 1712-1720en_HK
dc.identifier.issn0969-7128en_HK
dc.identifier.urihttp://hdl.handle.net/10722/163132-
dc.description.abstractGene transfer into the peritoneal cavity by nonviral methods may provide an effective therapeutic approach for peritoneal diseases. Herein, we investigated the feasibility and the effectiveness of ultrasound-microbubble-mediated delivery of naked plasmid DNA into the peritoneal cavity in rats. Following the intraperitoneal or the intravenous administration of a mixture of plasmid DNA (100 μg) and ultrasound contrast agent microbubbles, an ultrasound transducer was applied on the abdominal wall. The reporter pTRE plasmid encoding Smad7 was used to evaluate transfection efficiency. Smad7 expression was induced by doxycycline in drinking water. We detected less than 10% apoptotic cells and no inflammatory reaction in peritoneal tissues following the ultrasound-microbubble-mediated transfection. More importantly, the insonation significantly improved the transfection efficiency in peritoneal tissues. The transfection efficiency by intraperitoneal delivery route was higher than the intravenous route. The reporter gene, pTRE-Smad7, was readily detected in the parietal peritoneum, mesentery, greater omentum and adipose tissue. The peak of transgene expression occurred 2 days after transfection and the transgene expression diminished in a time-dependent manner thereafter. Overall, the effectiveness and simplicity of the ultrasound-microbubble-mediated system may provide a promising nonviral means for improving gene delivery for treating peritoneal diseases in vivo.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gten_HK
dc.relation.ispartofGene Therapyen_HK
dc.subject.meshAlbuminsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshContrast Mediaen_US
dc.subject.meshDna - Administration & Dosageen_US
dc.subject.meshFluorocarbonsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGene Therapy - Methodsen_US
dc.subject.meshGenetic Vectors - Administration & Dosageen_US
dc.subject.meshInjections, Intraperitonealen_US
dc.subject.meshInjections, Intravenousen_US
dc.subject.meshMicrobubblesen_US
dc.subject.meshPeritoneum - Pathologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSmad7 Protein - Analysis - Geneticsen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTransfection - Methodsen_US
dc.subject.meshTransgenesen_US
dc.subject.meshUltrasonicsen_US
dc.titleUltrasound-contrast agent mediated naked gene delivery in the peritoneal cavity of adult raten_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.gt.3303040en_HK
dc.identifier.pmid17960163-
dc.identifier.scopuseid_2-s2.0-36949024942en_HK
dc.identifier.hkuros147908-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36949024942&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume14en_HK
dc.identifier.issue24en_HK
dc.identifier.spage1712en_HK
dc.identifier.epage1720en_HK
dc.identifier.isiWOS:000251392700006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGuo, H=55468645700en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridChan, LYY=55182644100en_HK
dc.identifier.scopusauthoridTsang, AWL=7006979244en_HK
dc.identifier.scopusauthoridLam, MF=35300050600en_HK
dc.identifier.scopusauthoridLan, HY=7102710832en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.citeulike1825861-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats