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Article: PI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell lines
Title | PI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell lines |
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Authors | |
Issue Date | 2007 |
Citation | Journal Of Molecular Signaling, 2007, v. 2, article no. 9 How to Cite? |
Abstract | Background: We have previously derived highly similar lineage-restricted stem cell lines, RoSH and E-RoSH cell lines from mouse embryos and CD9hi SSEA-1- differentiated mouse embryonic stem cells, respectively. These cell lines are not pluripotent and differentiate readily into endothelial cells in vitro and in vivo. Results: We investigated the signaling pathway that maintains proliferation of these cells in an undifferentiated state, and demonstrate that PI3 K/Akt/mTOR, but not Raf/MEK/Erk, signaling in these cells was active during proliferation and was downregulated during endothelial differentiation. Inhibition of PI3 K/Akt/mTOR signaling, but not Raf/MEK/Erk, reduced proliferation and induced expression of endothelial specific proteins. During differentiation or inhibition of PI3 K/Akt/mTOR signaling, cyclinD2 transcript abundance in ribosome-enriched RNA but not in total RNA was reduced with a corresponding reduction in protein level. In contrast, transcript abundance of endothelial-specific genes e.g. Kdr, Tek and Pdgfrα in ribosome-enriched RNA fraction was not reduced and their protein levels were increased. Together these observations suggested that translational control mediated by PI3K/Akt/mTOR signaling was critical in regulating proliferation and endothelial differentiation of lineage-restricted RoSH-like stem cell lines. Conclusion: This study highlights translation regulation as a critical regulatory mechanism during proliferation and differentiation in stem cells. © 2007 Que et al; licensee BioMed Central Ltd. |
Persistent Identifier | http://hdl.handle.net/10722/163124 |
ISSN | 2023 SCImago Journal Rankings: 0.111 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Que, J | en_US |
dc.contributor.author | Lian, Q | en_US |
dc.contributor.author | El Oakley, RM | en_US |
dc.contributor.author | Lim, B | en_US |
dc.contributor.author | Lim, SK | en_US |
dc.date.accessioned | 2012-09-05T05:27:55Z | - |
dc.date.available | 2012-09-05T05:27:55Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Journal Of Molecular Signaling, 2007, v. 2, article no. 9 | en_US |
dc.identifier.issn | 1750-2187 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/163124 | - |
dc.description.abstract | Background: We have previously derived highly similar lineage-restricted stem cell lines, RoSH and E-RoSH cell lines from mouse embryos and CD9hi SSEA-1- differentiated mouse embryonic stem cells, respectively. These cell lines are not pluripotent and differentiate readily into endothelial cells in vitro and in vivo. Results: We investigated the signaling pathway that maintains proliferation of these cells in an undifferentiated state, and demonstrate that PI3 K/Akt/mTOR, but not Raf/MEK/Erk, signaling in these cells was active during proliferation and was downregulated during endothelial differentiation. Inhibition of PI3 K/Akt/mTOR signaling, but not Raf/MEK/Erk, reduced proliferation and induced expression of endothelial specific proteins. During differentiation or inhibition of PI3 K/Akt/mTOR signaling, cyclinD2 transcript abundance in ribosome-enriched RNA but not in total RNA was reduced with a corresponding reduction in protein level. In contrast, transcript abundance of endothelial-specific genes e.g. Kdr, Tek and Pdgfrα in ribosome-enriched RNA fraction was not reduced and their protein levels were increased. Together these observations suggested that translational control mediated by PI3K/Akt/mTOR signaling was critical in regulating proliferation and endothelial differentiation of lineage-restricted RoSH-like stem cell lines. Conclusion: This study highlights translation regulation as a critical regulatory mechanism during proliferation and differentiation in stem cells. © 2007 Que et al; licensee BioMed Central Ltd. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Molecular Signaling | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | PI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell lines | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lian, Q:qzlian@hkucc.hku.hk | en_US |
dc.identifier.authority | Lian, Q=rp00267 | en_US |
dc.description.nature | published_or_final_version | en_US |
dc.identifier.doi | 10.1186/1750-2187-2-9 | en_US |
dc.identifier.scopus | eid_2-s2.0-35649016567 | en_US |
dc.identifier.hkuros | 204419 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-35649016567&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 2 | en_US |
dc.identifier.scopusauthorid | Que, J=7003618579 | en_US |
dc.identifier.scopusauthorid | Lian, Q=7003399023 | en_US |
dc.identifier.scopusauthorid | El Oakley, RM=7003568099 | en_US |
dc.identifier.scopusauthorid | Lim, B=7201984111 | en_US |
dc.identifier.scopusauthorid | Lim, SK=7404080956 | en_US |
dc.identifier.issnl | 1750-2187 | - |