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Article: PI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell lines

TitlePI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell lines
Authors
Issue Date2007
Citation
Journal Of Molecular Signaling, 2007, v. 2, article no. 9 How to Cite?
AbstractBackground: We have previously derived highly similar lineage-restricted stem cell lines, RoSH and E-RoSH cell lines from mouse embryos and CD9hi SSEA-1- differentiated mouse embryonic stem cells, respectively. These cell lines are not pluripotent and differentiate readily into endothelial cells in vitro and in vivo. Results: We investigated the signaling pathway that maintains proliferation of these cells in an undifferentiated state, and demonstrate that PI3 K/Akt/mTOR, but not Raf/MEK/Erk, signaling in these cells was active during proliferation and was downregulated during endothelial differentiation. Inhibition of PI3 K/Akt/mTOR signaling, but not Raf/MEK/Erk, reduced proliferation and induced expression of endothelial specific proteins. During differentiation or inhibition of PI3 K/Akt/mTOR signaling, cyclinD2 transcript abundance in ribosome-enriched RNA but not in total RNA was reduced with a corresponding reduction in protein level. In contrast, transcript abundance of endothelial-specific genes e.g. Kdr, Tek and Pdgfrα in ribosome-enriched RNA fraction was not reduced and their protein levels were increased. Together these observations suggested that translational control mediated by PI3K/Akt/mTOR signaling was critical in regulating proliferation and endothelial differentiation of lineage-restricted RoSH-like stem cell lines. Conclusion: This study highlights translation regulation as a critical regulatory mechanism during proliferation and differentiation in stem cells. © 2007 Que et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/163124
ISSN
2015 SCImago Journal Rankings: 1.705
References

 

DC FieldValueLanguage
dc.contributor.authorQue, Jen_US
dc.contributor.authorLian, Qen_US
dc.contributor.authorEl Oakley, RMen_US
dc.contributor.authorLim, Ben_US
dc.contributor.authorLim, SKen_US
dc.date.accessioned2012-09-05T05:27:55Z-
dc.date.available2012-09-05T05:27:55Z-
dc.date.issued2007en_US
dc.identifier.citationJournal Of Molecular Signaling, 2007, v. 2, article no. 9en_US
dc.identifier.issn1750-2187en_US
dc.identifier.urihttp://hdl.handle.net/10722/163124-
dc.description.abstractBackground: We have previously derived highly similar lineage-restricted stem cell lines, RoSH and E-RoSH cell lines from mouse embryos and CD9hi SSEA-1- differentiated mouse embryonic stem cells, respectively. These cell lines are not pluripotent and differentiate readily into endothelial cells in vitro and in vivo. Results: We investigated the signaling pathway that maintains proliferation of these cells in an undifferentiated state, and demonstrate that PI3 K/Akt/mTOR, but not Raf/MEK/Erk, signaling in these cells was active during proliferation and was downregulated during endothelial differentiation. Inhibition of PI3 K/Akt/mTOR signaling, but not Raf/MEK/Erk, reduced proliferation and induced expression of endothelial specific proteins. During differentiation or inhibition of PI3 K/Akt/mTOR signaling, cyclinD2 transcript abundance in ribosome-enriched RNA but not in total RNA was reduced with a corresponding reduction in protein level. In contrast, transcript abundance of endothelial-specific genes e.g. Kdr, Tek and Pdgfrα in ribosome-enriched RNA fraction was not reduced and their protein levels were increased. Together these observations suggested that translational control mediated by PI3K/Akt/mTOR signaling was critical in regulating proliferation and endothelial differentiation of lineage-restricted RoSH-like stem cell lines. Conclusion: This study highlights translation regulation as a critical regulatory mechanism during proliferation and differentiation in stem cells. © 2007 Que et al; licensee BioMed Central Ltd.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Molecular Signalingen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titlePI3 K/Akt/mTOR-mediated translational control regulates proliferation and differentiation of lineage-restricted RoSH stem cell linesen_US
dc.typeArticleen_US
dc.identifier.emailLian, Q:qzlian@hkucc.hku.hken_US
dc.identifier.authorityLian, Q=rp00267en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1186/1750-2187-2-9en_US
dc.identifier.scopuseid_2-s2.0-35649016567en_US
dc.identifier.hkuros204419-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35649016567&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume2en_US
dc.identifier.scopusauthoridQue, J=7003618579en_US
dc.identifier.scopusauthoridLian, Q=7003399023en_US
dc.identifier.scopusauthoridEl Oakley, RM=7003568099en_US
dc.identifier.scopusauthoridLim, B=7201984111en_US
dc.identifier.scopusauthoridLim, SK=7404080956en_US

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