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Article: Elucidating the secretion proteome of human embryonic stem cell-derived mesenchymal stem cells

TitleElucidating the secretion proteome of human embryonic stem cell-derived mesenchymal stem cells
Authors
Issue Date2007
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc.. The Journal's web site is located at http://www.mcponline.org/
Citation
Molecular And Cellular Proteomics, 2007, v. 6 n. 10, p. 1680-1689 How to Cite?
AbstractTransplantation of mesenchymal stem cells (MSCs) has been used to treat a wide range of diseases, and the mechanism of action is postulated to be mediated by either differentiation into functional reparative cells that replace injured tissues or secretion of paracrine factors that promote tissue repair. To complement earlier studies that identified some of the paracrine factors, we profiled the paracrine proteome to better assess the relevance of MSC paracrine factors to the wide spectrum of MSC-mediated therapeutic effects. To evaluate the therapeutic potential of the MSC paracrine proteome, a chemically defined serum-free culture medium was conditioned by MSCs derived from human embryonic stem cells using a clinically compliant protocol. The conditioned medium was analyzed by multidimensional protein identification technology and cytokine antibody array analysis and revealed the presence of 201 unique gone products. 86-88% of these gene products had detectable transcript levels by microarray or quantitative RT-PCR assays. Computational analysis predicted that these gene products will significantly drive three major groups of biological processes: metabolism, defense response, and tissue differentiation including vascularization, hematopoiesis, and skeletal development. It also predicted that the 201 gene products activate important signaling pathways in cardiovascular biology, bone development, and hematopoiesis such as Jak-STAT, MAPK, Toll-like receptor, transforming growth factor-β, and mTOR (mammalian target of rapamycin) signaling pathways. This study identified a large number of MSC secretory products that have the potential to act as paracrine modulators of tissue repair and replacement in diseases of the cardiovascular, hematopoietic, and skeletal tissues. Moreover our results suggest that human embryonic stem cell-derived MSC-conditioned medium has the potency to treat a variety of diseases in humans without cell transplantation. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/163123
ISSN
2015 Impact Factor: 5.912
2015 SCImago Journal Rankings: 3.213
References

 

DC FieldValueLanguage
dc.contributor.authorSze, SKen_US
dc.contributor.authorDe Kleijn, DPVen_US
dc.contributor.authorLai, RCen_US
dc.contributor.authorTan, EKWen_US
dc.contributor.authorZhao, Hen_US
dc.contributor.authorYeo, KSen_US
dc.contributor.authorLow, TYen_US
dc.contributor.authorLian, Qen_US
dc.contributor.authorLee, CNen_US
dc.contributor.authorMitchell, Wen_US
dc.contributor.authorEl Oakley, RMen_US
dc.contributor.authorLim, SKen_US
dc.date.accessioned2012-09-05T05:27:54Z-
dc.date.available2012-09-05T05:27:54Z-
dc.date.issued2007en_US
dc.identifier.citationMolecular And Cellular Proteomics, 2007, v. 6 n. 10, p. 1680-1689en_US
dc.identifier.issn1535-9476en_US
dc.identifier.urihttp://hdl.handle.net/10722/163123-
dc.description.abstractTransplantation of mesenchymal stem cells (MSCs) has been used to treat a wide range of diseases, and the mechanism of action is postulated to be mediated by either differentiation into functional reparative cells that replace injured tissues or secretion of paracrine factors that promote tissue repair. To complement earlier studies that identified some of the paracrine factors, we profiled the paracrine proteome to better assess the relevance of MSC paracrine factors to the wide spectrum of MSC-mediated therapeutic effects. To evaluate the therapeutic potential of the MSC paracrine proteome, a chemically defined serum-free culture medium was conditioned by MSCs derived from human embryonic stem cells using a clinically compliant protocol. The conditioned medium was analyzed by multidimensional protein identification technology and cytokine antibody array analysis and revealed the presence of 201 unique gone products. 86-88% of these gene products had detectable transcript levels by microarray or quantitative RT-PCR assays. Computational analysis predicted that these gene products will significantly drive three major groups of biological processes: metabolism, defense response, and tissue differentiation including vascularization, hematopoiesis, and skeletal development. It also predicted that the 201 gene products activate important signaling pathways in cardiovascular biology, bone development, and hematopoiesis such as Jak-STAT, MAPK, Toll-like receptor, transforming growth factor-β, and mTOR (mammalian target of rapamycin) signaling pathways. This study identified a large number of MSC secretory products that have the potential to act as paracrine modulators of tissue repair and replacement in diseases of the cardiovascular, hematopoietic, and skeletal tissues. Moreover our results suggest that human embryonic stem cell-derived MSC-conditioned medium has the potency to treat a variety of diseases in humans without cell transplantation. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc.. The Journal's web site is located at http://www.mcponline.org/en_US
dc.relation.ispartofMolecular and Cellular Proteomicsen_US
dc.subject.meshChromatography, Liquiden_US
dc.subject.meshCulture Media, Conditioneden_US
dc.subject.meshEmbryonic Stem Cells - Cytologyen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGenome, Humanen_US
dc.subject.meshHumansen_US
dc.subject.meshMass Spectrometryen_US
dc.subject.meshMesenchymal Stem Cells - Cytology - Secretionen_US
dc.subject.meshMetabolic Networks And Pathwaysen_US
dc.subject.meshProtein Array Analysisen_US
dc.subject.meshProteome - Chemistry - Genetics - Secretionen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.titleElucidating the secretion proteome of human embryonic stem cell-derived mesenchymal stem cellsen_US
dc.typeArticleen_US
dc.identifier.emailLian, Q:qzlian@hkucc.hku.hken_US
dc.identifier.authorityLian, Q=rp00267en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1074/mcp.M600393-MCP200en_US
dc.identifier.pmid17565974-
dc.identifier.scopuseid_2-s2.0-35648974227en_US
dc.identifier.hkuros204422-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35648974227&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume6en_US
dc.identifier.issue10en_US
dc.identifier.spage1680en_US
dc.identifier.epage1689en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSze, SK=8842396900en_US
dc.identifier.scopusauthoridde Kleijn, DPV=7003654548en_US
dc.identifier.scopusauthoridLai, RC=22951110900en_US
dc.identifier.scopusauthoridTan, EKW=14012680600en_US
dc.identifier.scopusauthoridZhao, H=22952392700en_US
dc.identifier.scopusauthoridYeo, KS=22952467200en_US
dc.identifier.scopusauthoridLow, TY=7102899857en_US
dc.identifier.scopusauthoridLian, Q=7003399023en_US
dc.identifier.scopusauthoridLee, CN=12801077500en_US
dc.identifier.scopusauthoridMitchell, W=16064200700en_US
dc.identifier.scopusauthoridEl Oakley, RM=7003568099en_US
dc.identifier.scopusauthoridLim, SK=7404080956en_US

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