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Article: Frequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer
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TitleFrequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer
 
AuthorsCheng, YY3
Yu, J3
Wong, YP3
Man, EPS3
To, KF3
Jin, VX1
Li, J3
Tao, Q3
Sung, JJY3
Chan, FKL3
Leung, WK2 3
 
Issue Date2007
 
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
 
CitationBritish Journal Of Cancer, 2007, v. 97 n. 7, p. 895-901 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.bjc.6603968
 
AbstractThe role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P<0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer. © 2007 Cancer Research.
 
ISSN0007-0920
2013 Impact Factor: 4.817
 
DOIhttp://dx.doi.org/10.1038/sj.bjc.6603968
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCheng, YY
 
dc.contributor.authorYu, J
 
dc.contributor.authorWong, YP
 
dc.contributor.authorMan, EPS
 
dc.contributor.authorTo, KF
 
dc.contributor.authorJin, VX
 
dc.contributor.authorLi, J
 
dc.contributor.authorTao, Q
 
dc.contributor.authorSung, JJY
 
dc.contributor.authorChan, FKL
 
dc.contributor.authorLeung, WK
 
dc.date.accessioned2012-09-05T05:27:51Z
 
dc.date.available2012-09-05T05:27:51Z
 
dc.date.issued2007
 
dc.description.abstractThe role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P<0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer. © 2007 Cancer Research.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBritish Journal Of Cancer, 2007, v. 97 n. 7, p. 895-901 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.bjc.6603968
 
dc.identifier.citeulike1645977
 
dc.identifier.doihttp://dx.doi.org/10.1038/sj.bjc.6603968
 
dc.identifier.epage901
 
dc.identifier.issn0007-0920
2013 Impact Factor: 4.817
 
dc.identifier.issue7
 
dc.identifier.pmid17848950
 
dc.identifier.scopuseid_2-s2.0-34948898013
 
dc.identifier.spage895
 
dc.identifier.urihttp://hdl.handle.net/10722/163117
 
dc.identifier.volume97
 
dc.languageeng
 
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofBritish Journal of Cancer
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdenocarcinoma - Genetics - Metabolism
 
dc.subject.meshAnimals
 
dc.subject.meshApoptosis
 
dc.subject.meshAzacitidine
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshCell Proliferation
 
dc.subject.meshDna Methylation
 
dc.subject.meshDna Primers - Chemistry
 
dc.subject.meshEpigenesis, Genetic
 
dc.subject.meshEye Proteins - Genetics - Metabolism
 
dc.subject.meshFlow Cytometry
 
dc.subject.meshGene Expression Regulation, Neoplastic
 
dc.subject.meshGene Silencing
 
dc.subject.meshHumans
 
dc.subject.meshIntercellular Signaling Peptides And Proteins - Genetics - Metabolism
 
dc.subject.meshMembrane Proteins - Genetics - Metabolism
 
dc.subject.meshMice
 
dc.subject.meshMice, Nude
 
dc.subject.meshPromoter Regions, Genetic
 
dc.subject.meshProto-Oncogene Proteins - Genetics - Metabolism
 
dc.subject.meshStomach Neoplasms - Genetics - Metabolism
 
dc.titleFrequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer
 
dc.typeArticle
 
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<contributor.author>Jin, VX</contributor.author>
<contributor.author>Li, J</contributor.author>
<contributor.author>Tao, Q</contributor.author>
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<description.abstract>The role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P&lt;0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer. &#169; 2007 Cancer Research.</description.abstract>
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Author Affiliations
  1. UC Davis
  2. Prince of Wales Hospital Hong Kong
  3. Chinese University of Hong Kong