Article: Frequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer

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TitleFrequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer
AuthorsCheng, YY3
Yu, J3
Wong, YP3
Man, EPS3
To, KF3
Jin, VX1
Li, J3
Tao, Q3
Sung, JJY3
Chan, FKL3
Leung, WK2 3
Issue Date2007
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
CitationBritish Journal Of Cancer, 2007, v. 97 n. 7, p. 895-901 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.bjc.6603968
AbstractThe role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P<0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer. © 2007 Cancer Research.
ISSN0007-0920
2011 Impact Factor: 5.042
2011 SCImago Journal Rankings: 0.561
DOIhttp://dx.doi.org/10.1038/sj.bjc.6603968
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorCheng, YY
dc.contributor.authorYu, J
dc.contributor.authorWong, YP
dc.contributor.authorMan, EPS
dc.contributor.authorTo, KF
dc.contributor.authorJin, VX
dc.contributor.authorLi, J
dc.contributor.authorTao, Q
dc.contributor.authorSung, JJY
dc.contributor.authorChan, FKL
dc.contributor.authorLeung, WK
dc.date.accessioned2012-09-05T05:27:51Z
dc.date.available2012-09-05T05:27:51Z
dc.date.issued2007
dc.description.abstractThe role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P<0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer. © 2007 Cancer Research.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBritish Journal Of Cancer, 2007, v. 97 n. 7, p. 895-901 [How to Cite?]
DOI: http://dx.doi.org/10.1038/sj.bjc.6603968
dc.identifier.citeulike1645977
dc.identifier.doihttp://dx.doi.org/10.1038/sj.bjc.6603968
dc.identifier.epage901
dc.identifier.issn0007-0920
2011 Impact Factor: 5.042
2011 SCImago Journal Rankings: 0.561
dc.identifier.issue7
dc.identifier.pmid17848950
dc.identifier.scopuseid_2-s2.0-34948898013
dc.identifier.spage895
dc.identifier.urihttp://hdl.handle.net/10722/163117
dc.identifier.volume97
dc.languageeng
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
dc.publisher.placeUnited Kingdom
dc.relation.ispartofBritish Journal of Cancer
dc.relation.referencesReferences in Scopus
dc.subject.meshAdenocarcinoma - Genetics - Metabolism
dc.subject.meshAnimals
dc.subject.meshApoptosis
dc.subject.meshAzacitidine
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Proliferation
dc.subject.meshDna Methylation
dc.subject.meshDna Primers - Chemistry
dc.subject.meshEpigenesis, Genetic
dc.subject.meshEye Proteins - Genetics - Metabolism
dc.subject.meshFlow Cytometry
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshGene Silencing
dc.subject.meshHumans
dc.subject.meshIntercellular Signaling Peptides And Proteins - Genetics - Metabolism
dc.subject.meshMembrane Proteins - Genetics - Metabolism
dc.subject.meshMice
dc.subject.meshMice, Nude
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshProto-Oncogene Proteins - Genetics - Metabolism
dc.subject.meshStomach Neoplasms - Genetics - Metabolism
dc.titleFrequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer
dc.typeArticle
Author Affiliations
  1. UC Davis
  2. Prince of Wales Hospital Hong Kong
  3. Chinese University of Hong Kong