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Article: Frequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer

TitleFrequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric cancer
Authors
Issue Date2007
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjc
Citation
British Journal Of Cancer, 2007, v. 97 n. 7, p. 895-901 How to Cite?
AbstractThe role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P<0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer. © 2007 Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/163117
ISSN
2014 Impact Factor: 4.836
2014 SCImago Journal Rankings: 2.205
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheng, YYen_US
dc.contributor.authorYu, Jen_US
dc.contributor.authorWong, YPen_US
dc.contributor.authorMan, EPSen_US
dc.contributor.authorTo, KFen_US
dc.contributor.authorJin, VXen_US
dc.contributor.authorLi, Jen_US
dc.contributor.authorTao, Qen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorChan, FKLen_US
dc.contributor.authorLeung, WKen_US
dc.date.accessioned2012-09-05T05:27:51Z-
dc.date.available2012-09-05T05:27:51Z-
dc.date.issued2007en_US
dc.identifier.citationBritish Journal Of Cancer, 2007, v. 97 n. 7, p. 895-901en_US
dc.identifier.issn0007-0920en_US
dc.identifier.urihttp://hdl.handle.net/10722/163117-
dc.description.abstractThe role of secreted frizzled-related protein (SFRP) genes in gastric cancer remains largely unknown. We determined the frequency and functional significance of SFRPs hypermethylation in human gastric cancer. The expression and methylation status of four SFRP members (SFRP1, 2, 4, and 5) in primary gastric cancer samples was screened. The biological effects of SFRP were analysed by flow cytometry, cell viability assay and in vivo tumour growth in nude mice. Among the four SFRPs, only SFRP2 was significantly downregulated in gastric cancer as compared to adjacent non-cancer samples (P<0.01). Promoter hypermethylation of SFRP2 was detected in 73.3% primary gastric cancer tissues, 37.5% of samples showing intestinal metaplasia and 20% adjacent normal gastric tissues. Bisulphite DNA sequencing confirmed the densely methylated SFRP2 promoter region. Demethylation treatment restored the expression of SFRP2 in gastric cancer cell lines. Forced expression of SFRP2 induced cell apoptosis, inhibited proliferation of gastric cancer cells and suppressed tumour growth in vivo. Moreover, methylated SFRP2 was detected in 66.7% of serum samples from cancer patients but not in normal controls. In conclusion, epigenetic inactivation of SFRP2 is a common and early event contributing to gastric carcinogenesis and may be a potential biomarker for gastric cancer. © 2007 Cancer Research.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bjcen_US
dc.relation.ispartofBritish Journal of Canceren_US
dc.subject.meshAdenocarcinoma - Genetics - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshAzacitidineen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshDna Methylationen_US
dc.subject.meshDna Primers - Chemistryen_US
dc.subject.meshEpigenesis, Geneticen_US
dc.subject.meshEye Proteins - Genetics - Metabolismen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshGene Silencingen_US
dc.subject.meshHumansen_US
dc.subject.meshIntercellular Signaling Peptides And Proteins - Genetics - Metabolismen_US
dc.subject.meshMembrane Proteins - Genetics - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProto-Oncogene Proteins - Genetics - Metabolismen_US
dc.subject.meshStomach Neoplasms - Genetics - Metabolismen_US
dc.titleFrequent epigenetic inactivation of secreted frizzled-related protein 2 (SFRP2) by promoter methylation in human gastric canceren_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.bjc.6603968en_US
dc.identifier.pmid17848950-
dc.identifier.scopuseid_2-s2.0-34948898013en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34948898013&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume97en_US
dc.identifier.issue7en_US
dc.identifier.spage895en_US
dc.identifier.epage901en_US
dc.identifier.isiWOS:000249882000010-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridCheng, YY=7404914973en_US
dc.identifier.scopusauthoridYu, J=35351306800en_US
dc.identifier.scopusauthoridWong, YP=24802808500en_US
dc.identifier.scopusauthoridMan, EPS=7004439159en_US
dc.identifier.scopusauthoridTo, KF=7101911940en_US
dc.identifier.scopusauthoridJin, VX=26636467700en_US
dc.identifier.scopusauthoridLi, J=36066246900en_US
dc.identifier.scopusauthoridTao, Q=7102578359en_US
dc.identifier.scopusauthoridSung, JJY=36847007300en_US
dc.identifier.scopusauthoridChan, FKL=7202586434en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.citeulike1645977-

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