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Article: Establishing clonal cell lines with endothelial-like potential from CD9hi, SSEA-1- cells in enbryonic stem cell-derived enbryoid bodies

TitleEstablishing clonal cell lines with endothelial-like potential from CD9hi, SSEA-1- cells in enbryonic stem cell-derived enbryoid bodies
Authors
Issue Date2006
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2006, v. 1 n. 1, article no. e6 How to Cite?
AbstractBackground. Differentiation of embryonic stem cells (ESCs) into specific cell types with minimal risk of teratoma formation could be efficiently directed by first reducing the differentiation potential of ESCs through the generation of clonal, self-renewing lineage-restricted stem cell lines. Efforts to isolate these stem cells are, however, mired in an impasse where the lack of purified lineage-restricted stem cells has hindered the identification of defining markers for these rare stem cells and, in turn, their isolation. Methodology/Principal Findings. We describe here a method for the isolation of clonal lineage-restricted cell lines with endothelial potential from ESCs through a combination of empirical and rational evidence-based methods. Using an empirical protocol that we have previously developed to generate embryo-derived RoSH lines with endothelial potential, we first generated E-RoSH lines from mouse ESC-derived embryoid bodies (EBs). Despite originating from different mouse strains, RoSH and E- RoSH lines have similar gene expression profiles (r2 = 0.93) while that between ERoSH and ESCs was 0.83. In silico gene expression analysis predicted that like RoSH cells, E-RoSH cells have an increased propensity to differentiate into vasculature. Unlike their parental ESCs, E-RoSH cells did not form teratomas and differentiate efficiently into endothelial-like cells in vivo and in vitro. Gene expression and FACS analysis revealed that RoSH and E-RoSH cells are CD9hi, SSEA-1 while ESCs are CD9lo, SSEA-1+. Isolation of CD9hi, SSEA-1 cells that constituted 1%-10% of EB-derived cultures generated an E-RoSH-like culture with an identical E-RoSH-like gene expression profile (r2 = 0.95) and a propensity to differentiate into endothelial-like cells. Conclusions. By combining empirical and rational evidence-based methods, we identified definitive selectable surface antigens for the isolation and propagation of lineage-restricted stem cells with endothelial-like potential from mouse ESCs. © 2006 Lian et al.
Persistent Identifierhttp://hdl.handle.net/10722/163113
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLian, Qen_US
dc.contributor.authorYeo, KSen_US
dc.contributor.authorQue, Jen_US
dc.contributor.authorTan, EKWen_US
dc.contributor.authorYu, Fen_US
dc.contributor.authorYin, Yen_US
dc.contributor.authorSaltoTellez, Men_US
dc.contributor.authorEl Oakley, RMen_US
dc.contributor.authorLim, SKen_US
dc.date.accessioned2012-09-05T05:27:46Z-
dc.date.available2012-09-05T05:27:46Z-
dc.date.issued2006en_US
dc.identifier.citationPlos One, 2006, v. 1 n. 1, article no. e6en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10722/163113-
dc.description.abstractBackground. Differentiation of embryonic stem cells (ESCs) into specific cell types with minimal risk of teratoma formation could be efficiently directed by first reducing the differentiation potential of ESCs through the generation of clonal, self-renewing lineage-restricted stem cell lines. Efforts to isolate these stem cells are, however, mired in an impasse where the lack of purified lineage-restricted stem cells has hindered the identification of defining markers for these rare stem cells and, in turn, their isolation. Methodology/Principal Findings. We describe here a method for the isolation of clonal lineage-restricted cell lines with endothelial potential from ESCs through a combination of empirical and rational evidence-based methods. Using an empirical protocol that we have previously developed to generate embryo-derived RoSH lines with endothelial potential, we first generated E-RoSH lines from mouse ESC-derived embryoid bodies (EBs). Despite originating from different mouse strains, RoSH and E- RoSH lines have similar gene expression profiles (r2 = 0.93) while that between ERoSH and ESCs was 0.83. In silico gene expression analysis predicted that like RoSH cells, E-RoSH cells have an increased propensity to differentiate into vasculature. Unlike their parental ESCs, E-RoSH cells did not form teratomas and differentiate efficiently into endothelial-like cells in vivo and in vitro. Gene expression and FACS analysis revealed that RoSH and E-RoSH cells are CD9hi, SSEA-1 while ESCs are CD9lo, SSEA-1+. Isolation of CD9hi, SSEA-1 cells that constituted 1%-10% of EB-derived cultures generated an E-RoSH-like culture with an identical E-RoSH-like gene expression profile (r2 = 0.95) and a propensity to differentiate into endothelial-like cells. Conclusions. By combining empirical and rational evidence-based methods, we identified definitive selectable surface antigens for the isolation and propagation of lineage-restricted stem cells with endothelial-like potential from mouse ESCs. © 2006 Lian et al.en_US
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_US
dc.relation.ispartofPLoS ONEen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd - Metabolismen_US
dc.subject.meshAntigens, Cd15 - Metabolismen_US
dc.subject.meshAntigens, Cd9en_US
dc.subject.meshCell Culture Techniques - Methodsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Separation - Methodsen_US
dc.subject.meshClone Cellsen_US
dc.subject.meshEmbryonic Stem Cells - Cytology - Immunology - Metabolism - Transplantationen_US
dc.subject.meshEndothelial Cells - Cytology - Immunology - Metabolismen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshMembrane Glycoproteins - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Sciden_US
dc.subject.meshOligonucleotide Array Sequence Analysisen_US
dc.titleEstablishing clonal cell lines with endothelial-like potential from CD9hi, SSEA-1- cells in enbryonic stem cell-derived enbryoid bodiesen_US
dc.typeArticleen_US
dc.identifier.emailLian, Q:qzlian@hkucc.hku.hken_US
dc.identifier.authorityLian, Q=rp00267en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1371/journal.pone.0000006en_US
dc.identifier.pmid17183690-
dc.identifier.pmcidPMC1762397-
dc.identifier.scopuseid_2-s2.0-34748876977en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34748876977&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1en_US
dc.identifier.issue1en_US
dc.identifier.spagearticle no. e6-
dc.identifier.epagearticle no. e6-
dc.identifier.isiWOS:000207443600006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLian, Q=7003399023en_US
dc.identifier.scopusauthoridYeo, KS=22952467200en_US
dc.identifier.scopusauthoridQue, J=7003618579en_US
dc.identifier.scopusauthoridTan, EKW=14012680600en_US
dc.identifier.scopusauthoridYu, F=7402822568en_US
dc.identifier.scopusauthoridYin, Y=7403273716en_US
dc.identifier.scopusauthoridSaltoTellez, M=7003434917en_US
dc.identifier.scopusauthoridEl Oakley, RM=7003568099en_US
dc.identifier.scopusauthoridLim, SK=7404080956en_US
dc.identifier.citeulike3093174-

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