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Article: Yeast cap binding complex impedes recruitment of cleavage factor IA to weak termination sites

TitleYeast cap binding complex impedes recruitment of cleavage factor IA to weak termination sites
Authors
Issue Date2007
Citation
Molecular And Cellular Biology, 2007, v. 27 n. 18, p. 6520-6531 How to Cite?
AbstractNuclear cap binding complex (CBC) is recruited cotranscriptionally and stimulates spliceosome assembly on nascent mRNAs; however, its possible functions in regulating transcription elongation or termination were not well understood. We show that, while CBC appears to be dispensable for normal rates and processivity of elongation by RNA polymerase II (Pol II), it plays a direct role in preventing polyadenylation at weak termination sites. Similarly to Npl3p, with which it interacts, CBC suppresses the weak terminator of the gal10-Δ56 mutant allele by impeding recruitment of termination factors Pcf11p and Rna15p (subunits of cleavage factor IA [CF IA]) and does so without influencing Npl3p occupancy at the termination site. Importantly, deletion of CBC subunits or NPL3 also increases termination at a naturally occurring weak poly(A) site in the RNA14 coding sequences. We also show that CBC is most likely recruited directly to the cap of nascent transcripts rather than interacting first with transcriptional activators or the phosphorylated C-terminal domain of Pol II. Thus, our findings illuminate the mechanism of CBC recruitment and extend its function in Saccharomyces cerevisiae beyond mRNA splicing and degradation of aberrant nuclear mRNAs to include regulation of CF IA recruitment at poly(A) selection sites.
Persistent Identifierhttp://hdl.handle.net/10722/163108
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, CMen_US
dc.contributor.authorQiu, Hen_US
dc.contributor.authorHu, Cen_US
dc.contributor.authorDong, Jen_US
dc.contributor.authorHinnebusch, AGen_US
dc.date.accessioned2012-09-05T05:27:42Z-
dc.date.available2012-09-05T05:27:42Z-
dc.date.issued2007en_US
dc.identifier.citationMolecular And Cellular Biology, 2007, v. 27 n. 18, p. 6520-6531en_US
dc.identifier.issn0270-7306en_US
dc.identifier.urihttp://hdl.handle.net/10722/163108-
dc.description.abstractNuclear cap binding complex (CBC) is recruited cotranscriptionally and stimulates spliceosome assembly on nascent mRNAs; however, its possible functions in regulating transcription elongation or termination were not well understood. We show that, while CBC appears to be dispensable for normal rates and processivity of elongation by RNA polymerase II (Pol II), it plays a direct role in preventing polyadenylation at weak termination sites. Similarly to Npl3p, with which it interacts, CBC suppresses the weak terminator of the gal10-Δ56 mutant allele by impeding recruitment of termination factors Pcf11p and Rna15p (subunits of cleavage factor IA [CF IA]) and does so without influencing Npl3p occupancy at the termination site. Importantly, deletion of CBC subunits or NPL3 also increases termination at a naturally occurring weak poly(A) site in the RNA14 coding sequences. We also show that CBC is most likely recruited directly to the cap of nascent transcripts rather than interacting first with transcriptional activators or the phosphorylated C-terminal domain of Pol II. Thus, our findings illuminate the mechanism of CBC recruitment and extend its function in Saccharomyces cerevisiae beyond mRNA splicing and degradation of aberrant nuclear mRNAs to include regulation of CF IA recruitment at poly(A) selection sites.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Biologyen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRna Cap-Binding Proteins - Metabolismen_US
dc.subject.meshRna, Fungal - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshSaccharomyces Cerevisiae - Genetics - Metabolismen_US
dc.subject.meshSaccharomyces Cerevisiae Proteins - Metabolismen_US
dc.subject.meshMrna Cleavage And Polyadenylation Factors - Metabolismen_US
dc.titleYeast cap binding complex impedes recruitment of cleavage factor IA to weak termination sitesen_US
dc.typeArticleen_US
dc.identifier.emailWong, CM:wispwong@hkucc.hku.hken_US
dc.identifier.authorityWong, CM=rp01489en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/MCB.00733-07en_US
dc.identifier.pmid17636014-
dc.identifier.scopuseid_2-s2.0-34548710303en_US
dc.identifier.hkuros205104-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548710303&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume27en_US
dc.identifier.issue18en_US
dc.identifier.spage6520en_US
dc.identifier.epage6531en_US
dc.identifier.eissn1098-5549-
dc.identifier.isiWOS:000249319200022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, CM=18134632400en_US
dc.identifier.scopusauthoridQiu, H=7201608807en_US
dc.identifier.scopusauthoridHu, C=7404570409en_US
dc.identifier.scopusauthoridDong, J=7403365571en_US
dc.identifier.scopusauthoridHinnebusch, AG=7005728566en_US

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