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Article: Antitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma 1 with lentivirus-delivered siRNA

TitleAntitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma 1 with lentivirus-delivered siRNA
Authors
Issue Date2007
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2007, v. 120 n. 9, p. 749-754 How to Cite?
AbstractBackground: In most colorectal carcinomas, the level of phospholipase C (PLC)-gamma 1 expression is greatly elevated. Increased expression of PLC-gamma 1 may play an important role in colon carcinogenesis, but the mechanism is not well known. The aim of this study was to evaluate the role of PLC-gamma 1 in colon carcinogenesis by using recombinant lentivirus that stably suppressed the PLC-gamma 1 expression in human colorectal carcinoma LoVo cells. Methods: Recombinant lentivirus producing PLC-gamma 1 siRNA were prepared. After LoVo cells were transduced by each lentivirus, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC-gamma 1 were examined by Western-blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis, and the effects of the lentivirus on the call adhesion, migration and apoptosis were analyzed. Results: Stable LoVo cell line deficient in PLC-gamma 1, was established. Notably, PLC-gamma 1 was silenced without affecting the levels of other subtypes of PLC so that the role of PLC-gamma 1 in colon carcinogenesis could be examined. Silencing of endogenous PLC-gamma 1 resulted in efficient inhibition of the adhesion and migration of LoVo cells in vitro and a great increase of 5-fluorouracil induced apoptosis (30%-40%) of LoVo cells. Conclusion: PLC-gamma 1 may play an important role in metastasis and anti-apoptosis in human colorectal carcinomas. © 2005-2008 Chinese Medical Journal, All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/163082
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTan, Len_US
dc.contributor.authorXiao, BXen_US
dc.contributor.authorZeng, WSen_US
dc.contributor.authorLin, Jen_US
dc.contributor.authorZou, ZPen_US
dc.contributor.authorXu, AMen_US
dc.contributor.authorLuo, SQen_US
dc.date.accessioned2012-09-05T05:27:22Z-
dc.date.available2012-09-05T05:27:22Z-
dc.date.issued2007en_US
dc.identifier.citationChinese Medical Journal, 2007, v. 120 n. 9, p. 749-754en_US
dc.identifier.issn0366-6999en_US
dc.identifier.urihttp://hdl.handle.net/10722/163082-
dc.description.abstractBackground: In most colorectal carcinomas, the level of phospholipase C (PLC)-gamma 1 expression is greatly elevated. Increased expression of PLC-gamma 1 may play an important role in colon carcinogenesis, but the mechanism is not well known. The aim of this study was to evaluate the role of PLC-gamma 1 in colon carcinogenesis by using recombinant lentivirus that stably suppressed the PLC-gamma 1 expression in human colorectal carcinoma LoVo cells. Methods: Recombinant lentivirus producing PLC-gamma 1 siRNA were prepared. After LoVo cells were transduced by each lentivirus, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC-gamma 1 were examined by Western-blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis, and the effects of the lentivirus on the call adhesion, migration and apoptosis were analyzed. Results: Stable LoVo cell line deficient in PLC-gamma 1, was established. Notably, PLC-gamma 1 was silenced without affecting the levels of other subtypes of PLC so that the role of PLC-gamma 1 in colon carcinogenesis could be examined. Silencing of endogenous PLC-gamma 1 resulted in efficient inhibition of the adhesion and migration of LoVo cells in vitro and a great increase of 5-fluorouracil induced apoptosis (30%-40%) of LoVo cells. Conclusion: PLC-gamma 1 may play an important role in metastasis and anti-apoptosis in human colorectal carcinomas. © 2005-2008 Chinese Medical Journal, All Rights Reserved.en_US
dc.languageengen_US
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_US
dc.relation.ispartofChinese Medical Journalen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCell Adhesionen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshColorectal Neoplasms - Pathology - Therapyen_US
dc.subject.meshFluorouracil - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLaminin - Antagonists & Inhibitors - Geneticsen_US
dc.subject.meshLentivirus - Geneticsen_US
dc.subject.meshPhospholipase C Gamma - Antagonists & Inhibitors - Genetics - Physiologyen_US
dc.subject.meshRna, Small Interfering - Therapeutic Useen_US
dc.titleAntitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma 1 with lentivirus-delivered siRNAen_US
dc.typeArticleen_US
dc.identifier.emailXu, AM:amxu@hkucc.hku.hken_US
dc.identifier.authorityXu, AM=rp00485en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid17531112-
dc.identifier.scopuseid_2-s2.0-34248660287en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34248660287&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume120en_US
dc.identifier.issue9en_US
dc.identifier.spage749en_US
dc.identifier.epage754en_US
dc.identifier.isiWOS:000246829300003-
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridTan, L=24067823200en_US
dc.identifier.scopusauthoridXiao, BX=15844571300en_US
dc.identifier.scopusauthoridZeng, WS=9040099600en_US
dc.identifier.scopusauthoridLin, J=36079533900en_US
dc.identifier.scopusauthoridZou, ZP=7201440180en_US
dc.identifier.scopusauthoridXu, AM=7202655409en_US
dc.identifier.scopusauthoridLuo, SQ=7401985712en_US

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