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Article: Effects of neonatal maternal separation on neurochemical and sensory response to colonic distension in a rat model of irritable bowel syndrome

TitleEffects of neonatal maternal separation on neurochemical and sensory response to colonic distension in a rat model of irritable bowel syndrome
Authors
Keywords5-HT
c-fos
Neonatal maternal separation
Serotonin
Visceral hyperalgesia
Issue Date2007
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/
Citation
American Journal Of Physiology - Gastrointestinal And Liver Physiology, 2007, v. 292 n. 3, p. G849-G856 How to Cite?
AbstractEarly life stress has been implicated as a risk factor for irritable bowel syndrome (IBS). We studied the effect of neonatal maternal separation on the visceromotor response and the expression of c-fos, 5-HT, and its receptors/transporters along the brain-gut axis in an animal model of IBS. Male neonatal Sprague-Dawley rats were randomly assigned to a 3-h daily maternal separation (MS) or nonhandling (NH) on postnatal days 2-21. Colorectal balloon distention (CRD) was performed for assessment of abdominal withdrawal reflex as a surrogate marker of visceral pain. Tissues from dorsal raphe nucleus in midbrain, lumbar-sacral cord, and distal colon were harvested for semiquantitative analysis of c-fos and 5-HT. The expression of 5-HT expression, 5-HT3 receptors, and 5-HT transporter were analyzed by RT-PCR. Pain threshold was significantly lower in MS than NH rats. The abdominal withdrawal reflex score in response to CRD in MS rats was significantly higher with distension pressures of 40, 60, and 80 mmHg. In MS rats, the number of c-fos-like immunoreactive nuclei at dorsal horn of lumbar-sacral spinal cord increased significantly after CRD. 5-HT content in the spinal cord of MS rats was significant higher. In the colon, both 5-HT-positive cell number and 5-HT content were comparable between MS and NH groups before CRD. Post-CRD only MS rats had significant increase in 5-HT content. Protein and mRNA expression levels of 5-HT3 receptors and 5-HT transporter were similar in MS and NH rats. Neonatal maternal separation stress predisposes rats to exaggerated neurochemical responses and visceral hyperalgesia in colon mimicking IBS. Copyright © 2007 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/163068
ISSN
2015 Impact Factor: 3.297
2015 SCImago Journal Rankings: 1.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRen, THen_HK
dc.contributor.authorWu, Jen_HK
dc.contributor.authorYew, Den_HK
dc.contributor.authorZiea, Een_HK
dc.contributor.authorLao, Len_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorBerman, Ben_HK
dc.contributor.authorHu, PJen_HK
dc.contributor.authorSung, JJYen_HK
dc.date.accessioned2012-09-05T05:27:11Z-
dc.date.available2012-09-05T05:27:11Z-
dc.date.issued2007en_HK
dc.identifier.citationAmerican Journal Of Physiology - Gastrointestinal And Liver Physiology, 2007, v. 292 n. 3, p. G849-G856en_HK
dc.identifier.issn0193-1857en_HK
dc.identifier.urihttp://hdl.handle.net/10722/163068-
dc.description.abstractEarly life stress has been implicated as a risk factor for irritable bowel syndrome (IBS). We studied the effect of neonatal maternal separation on the visceromotor response and the expression of c-fos, 5-HT, and its receptors/transporters along the brain-gut axis in an animal model of IBS. Male neonatal Sprague-Dawley rats were randomly assigned to a 3-h daily maternal separation (MS) or nonhandling (NH) on postnatal days 2-21. Colorectal balloon distention (CRD) was performed for assessment of abdominal withdrawal reflex as a surrogate marker of visceral pain. Tissues from dorsal raphe nucleus in midbrain, lumbar-sacral cord, and distal colon were harvested for semiquantitative analysis of c-fos and 5-HT. The expression of 5-HT expression, 5-HT3 receptors, and 5-HT transporter were analyzed by RT-PCR. Pain threshold was significantly lower in MS than NH rats. The abdominal withdrawal reflex score in response to CRD in MS rats was significantly higher with distension pressures of 40, 60, and 80 mmHg. In MS rats, the number of c-fos-like immunoreactive nuclei at dorsal horn of lumbar-sacral spinal cord increased significantly after CRD. 5-HT content in the spinal cord of MS rats was significant higher. In the colon, both 5-HT-positive cell number and 5-HT content were comparable between MS and NH groups before CRD. Post-CRD only MS rats had significant increase in 5-HT content. Protein and mRNA expression levels of 5-HT3 receptors and 5-HT transporter were similar in MS and NH rats. Neonatal maternal separation stress predisposes rats to exaggerated neurochemical responses and visceral hyperalgesia in colon mimicking IBS. Copyright © 2007 the American Physiological Society.en_HK
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpgi.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Gastrointestinal and Liver Physiologyen_HK
dc.subject5-HTen_HK
dc.subjectc-fosen_HK
dc.subjectNeonatal maternal separationen_HK
dc.subjectSerotoninen_HK
dc.subjectVisceral hyperalgesiaen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshBalloon Dilationen_US
dc.subject.meshCholangitis - Pathologyen_US
dc.subject.meshColon - Innervation - Metabolism - Physiopathologyen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshIrritable Bowel Syndrome - Metabolism - Physiopathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMaternal Deprivationen_US
dc.subject.meshPain Thresholden_US
dc.subject.meshPressureen_US
dc.subject.meshProto-Oncogene Proteins C-Fos - Metabolismen_US
dc.subject.meshRaphe Nuclei - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Serotonin, 5-Ht3 - Genetics - Metabolismen_US
dc.subject.meshReflex - Physiologyen_US
dc.subject.meshSerotonin - Metabolismen_US
dc.subject.meshSerotonin Plasma Membrane Transport Proteins - Genetics - Metabolismen_US
dc.subject.meshSpinal Cord - Metabolismen_US
dc.subject.meshStress, Psychological - Metabolism - Physiopathologyen_US
dc.titleEffects of neonatal maternal separation on neurochemical and sensory response to colonic distension in a rat model of irritable bowel syndromeen_HK
dc.typeArticleen_HK
dc.identifier.emailLao, L: lxlao1@hku.hken_HK
dc.identifier.emailLeung, WK: hku75407@hku.hken_HK
dc.identifier.authorityLao, L=rp01784en_HK
dc.identifier.authorityLeung, WK=rp01479en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1152/ajpgi.00400.2006en_HK
dc.identifier.pmid17110521-
dc.identifier.scopuseid_2-s2.0-33947315932en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947315932&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume292en_HK
dc.identifier.issue3en_HK
dc.identifier.spageG849en_HK
dc.identifier.epageG856en_HK
dc.identifier.isiWOS:000244787200017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRen, TH=16048242100en_HK
dc.identifier.scopusauthoridWu, J=7409253910en_HK
dc.identifier.scopusauthoridYew, D=7007034694en_HK
dc.identifier.scopusauthoridZiea, E=6507982323en_HK
dc.identifier.scopusauthoridLao, L=7005681883en_HK
dc.identifier.scopusauthoridLeung, WK=7201504523en_HK
dc.identifier.scopusauthoridBerman, B=35458606800en_HK
dc.identifier.scopusauthoridHu, PJ=7201989582en_HK
dc.identifier.scopusauthoridSung, JJY=35405352400en_HK

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