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Article: Derivation of clinically compliant MSCs from CD105+, CD24-differentiated human ESCs

TitleDerivation of clinically compliant MSCs from CD105+, CD24-differentiated human ESCs
Authors
Issue Date2007
PublisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.com
Citation
Stem Cells, 2007, v. 25 n. 2, p. 425-436 How to Cite?
AbstractAdult tissue-derived mesenchymal stem cells (MSCs) have demonstrated therapeutic efficacy in treating diseases or repairing damaged tissues through mechanisms thought to be mediated by either cell replacement or secretion of paracrine factors. Characterized, self-renewing human ESCs could potentially be an invariable source of consistently uniform MSCs for therapeutic applications. Here we describe a clinically relevant and reproducible manner of generating identical batches of hESC-derived MSC (hESC-MSC) cultures that circumvents exposure to virus, mouse cells, or serum. Trypsinization and propagation of HuES9 or H1 hESCs in feeder- and serum-free selection media generated three polyclonal, karyotypically stable, and phenotypically MSC-like cultures that do not express pluripotency-associated markers but displayed MSC-like surface antigens and gene expression profile. They differentiate into adipocytes, osteocytes, and chondrocytes in vitro. Gene expression and fluorescence- activated cell sorter analysis identified CD105 and CD24 as highly expressed antigens on hESC-MSCs and hESCs, respectively. CD105+, CD24- monoclonal isolates have a typical MSC gene expression profiles and were identical to each other with a highly correlated gene expression profile (r 2 > .90). We have developed a protocol to reproducibly generate clinically compliant and identical hESC-MSC cultures. ©AlphaMed Press.
Persistent Identifierhttp://hdl.handle.net/10722/163062
ISSN
2015 Impact Factor: 5.902
2015 SCImago Journal Rankings: 3.438
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLian, Qen_US
dc.contributor.authorLye, Een_US
dc.contributor.authorKeng, SYen_US
dc.contributor.authorTan, EKWen_US
dc.contributor.authorSaltoTellez, Men_US
dc.contributor.authorTong, MLen_US
dc.contributor.authorPalanisamy, Nen_US
dc.contributor.authorOakley, RMWEen_US
dc.contributor.authorEng, HLen_US
dc.contributor.authorLim, Ben_US
dc.contributor.authorLim, SKen_US
dc.date.accessioned2012-09-05T05:27:07Z-
dc.date.available2012-09-05T05:27:07Z-
dc.date.issued2007en_US
dc.identifier.citationStem Cells, 2007, v. 25 n. 2, p. 425-436en_US
dc.identifier.issn1066-5099en_US
dc.identifier.urihttp://hdl.handle.net/10722/163062-
dc.description.abstractAdult tissue-derived mesenchymal stem cells (MSCs) have demonstrated therapeutic efficacy in treating diseases or repairing damaged tissues through mechanisms thought to be mediated by either cell replacement or secretion of paracrine factors. Characterized, self-renewing human ESCs could potentially be an invariable source of consistently uniform MSCs for therapeutic applications. Here we describe a clinically relevant and reproducible manner of generating identical batches of hESC-derived MSC (hESC-MSC) cultures that circumvents exposure to virus, mouse cells, or serum. Trypsinization and propagation of HuES9 or H1 hESCs in feeder- and serum-free selection media generated three polyclonal, karyotypically stable, and phenotypically MSC-like cultures that do not express pluripotency-associated markers but displayed MSC-like surface antigens and gene expression profile. They differentiate into adipocytes, osteocytes, and chondrocytes in vitro. Gene expression and fluorescence- activated cell sorter analysis identified CD105 and CD24 as highly expressed antigens on hESC-MSCs and hESCs, respectively. CD105+, CD24- monoclonal isolates have a typical MSC gene expression profiles and were identical to each other with a highly correlated gene expression profile (r 2 > .90). We have developed a protocol to reproducibly generate clinically compliant and identical hESC-MSC cultures. ©AlphaMed Press.en_US
dc.languageengen_US
dc.publisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.comen_US
dc.relation.ispartofStem Cellsen_US
dc.subject.meshAdipogenesisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd - Genetics - Immunology - Metabolismen_US
dc.subject.meshAntigens, Cd24 - Genetics - Immunology - Metabolismen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Separationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChondrogenesisen_US
dc.subject.meshEmbryonic Stem Cells - Cytology - Metabolism - Transplantationen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHumansen_US
dc.subject.meshMesenchymal Stem Cells - Cytology - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshOsteogenesisen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshReceptors, Cell Surface - Genetics - Immunology - Metabolismen_US
dc.subject.meshStem Cell Transplantationen_US
dc.titleDerivation of clinically compliant MSCs from CD105+, CD24-differentiated human ESCsen_US
dc.typeArticleen_US
dc.identifier.emailLian, Q:qzlian@hkucc.hku.hken_US
dc.identifier.authorityLian, Q=rp00267en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1634/stemcells.2006-0420en_US
dc.identifier.pmid17053208en_US
dc.identifier.scopuseid_2-s2.0-33846937043en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846937043&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume25en_US
dc.identifier.issue2en_US
dc.identifier.spage425en_US
dc.identifier.epage436en_US
dc.identifier.eissn1549-4918-
dc.identifier.isiWOS:000244070600021-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLian, Q=7003399023en_US
dc.identifier.scopusauthoridLye, E=15843514300en_US
dc.identifier.scopusauthoridKeng, SY=15843354200en_US
dc.identifier.scopusauthoridTan, EKW=14012680600en_US
dc.identifier.scopusauthoridSaltoTellez, M=7003434917en_US
dc.identifier.scopusauthoridTong, ML=15844545100en_US
dc.identifier.scopusauthoridPalanisamy, N=6603644701en_US
dc.identifier.scopusauthoridOakley, RMWE=7006484508en_US
dc.identifier.scopusauthoridEng, HL=8733545000en_US
dc.identifier.scopusauthoridLim, B=7201984111en_US
dc.identifier.scopusauthoridLim, SK=7404080956en_US

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