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Article: Induction of apoptosis and cell cycle arrest by a specific c-Jun NH 2-terminal kinase (JNK) inhibitor, SP-600125, in gastrointestinal cancers

TitleInduction of apoptosis and cell cycle arrest by a specific c-Jun NH 2-terminal kinase (JNK) inhibitor, SP-600125, in gastrointestinal cancers
Authors
KeywordsApoptosis
c-Jun NH2-terminal kinase
Cell cycle arrest
Cell viability
Gastrointestinal cancers
JNK inhibitor
Issue Date2006
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
Citation
Cancer Letters, 2006, v. 241 n. 2, p. 268-274 How to Cite?
AbstractThe c-Jun NH 2-terminal kinase (JNK) is activated in several tumor cell lines. The aim of this study was to determine the effects of SP-600125, a specific JNK inhibitor, on the viability, apoptosis, cell cycle distribution of gastrointestinal cancer cells, and the potential anti-tumor mechanisms. Three gastric cancer cell lines, AGS, BCG-823 and MKN-45, and three colorectal cancer cell lines, SW1116, COLO205 and HT-29, were used. Cells were treated with SP-600125, and cell viability, apoptosis and cell cycle distribution, caspase-3 activity, expression of JNK and apoptosis related proteins were detected. SP-600125 inhibited cell proliferation by 10-80% for the different cell lines, and increased apoptosis by 1.5-4.5 folds for COLO205, BCG-823, MKN-45, AGS cells. Caspase-8 and caspase-3 were involved in the induction of apoptosis. SP-600125 caused G2/M cell cycle arrest and elevation of cyclin B1 and p27 kip. The differential response in cells to SP-600125 was associated with the basal level of phosphorylated JNK2. It is concluded that SP-600125 inhibits proliferation, induces apoptosis and causes cell cycle arrest in gastrointestinal cancer cells, indicating that JNK inhibitors have an anti-tumor effect and are potential therapeutic agents for cancers. © 2005 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/163007
ISSN
2015 Impact Factor: 5.992
2015 SCImago Journal Rankings: 2.331
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXia, HHXen_US
dc.contributor.authorHe, Hen_US
dc.contributor.authorWang, JDen_US
dc.contributor.authorGu, Qen_US
dc.contributor.authorLin, MCMen_US
dc.contributor.authorZou, Ben_US
dc.contributor.authorYu, LFen_US
dc.contributor.authorSun, YWen_US
dc.contributor.authorChan, AOOen_US
dc.contributor.authorKung, HFen_US
dc.contributor.authorWong, BCYen_US
dc.date.accessioned2012-09-05T05:26:29Z-
dc.date.available2012-09-05T05:26:29Z-
dc.date.issued2006en_US
dc.identifier.citationCancer Letters, 2006, v. 241 n. 2, p. 268-274en_US
dc.identifier.issn0304-3835en_US
dc.identifier.urihttp://hdl.handle.net/10722/163007-
dc.description.abstractThe c-Jun NH 2-terminal kinase (JNK) is activated in several tumor cell lines. The aim of this study was to determine the effects of SP-600125, a specific JNK inhibitor, on the viability, apoptosis, cell cycle distribution of gastrointestinal cancer cells, and the potential anti-tumor mechanisms. Three gastric cancer cell lines, AGS, BCG-823 and MKN-45, and three colorectal cancer cell lines, SW1116, COLO205 and HT-29, were used. Cells were treated with SP-600125, and cell viability, apoptosis and cell cycle distribution, caspase-3 activity, expression of JNK and apoptosis related proteins were detected. SP-600125 inhibited cell proliferation by 10-80% for the different cell lines, and increased apoptosis by 1.5-4.5 folds for COLO205, BCG-823, MKN-45, AGS cells. Caspase-8 and caspase-3 were involved in the induction of apoptosis. SP-600125 caused G2/M cell cycle arrest and elevation of cyclin B1 and p27 kip. The differential response in cells to SP-600125 was associated with the basal level of phosphorylated JNK2. It is concluded that SP-600125 inhibits proliferation, induces apoptosis and causes cell cycle arrest in gastrointestinal cancer cells, indicating that JNK inhibitors have an anti-tumor effect and are potential therapeutic agents for cancers. © 2005 Elsevier Ireland Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canleten_US
dc.relation.ispartofCancer Lettersen_US
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.-
dc.subjectApoptosis-
dc.subjectc-Jun NH2-terminal kinase-
dc.subjectCell cycle arrest-
dc.subjectCell viability-
dc.subjectGastrointestinal cancers-
dc.subjectJNK inhibitor-
dc.subject.meshAnthracenes - Pharmacologyen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCaspases - Metabolismen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshEnzyme Inhibitors - Pharmacologyen_US
dc.subject.meshG2 Phase - Drug Effectsen_US
dc.subject.meshGastrointestinal Neoplasms - Drug Therapyen_US
dc.subject.meshHumansen_US
dc.subject.meshJnk Mitogen-Activated Protein Kinases - Antagonists & Inhibitors - Genetics - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleInduction of apoptosis and cell cycle arrest by a specific c-Jun NH 2-terminal kinase (JNK) inhibitor, SP-600125, in gastrointestinal cancersen_US
dc.typeArticleen_US
dc.identifier.emailWang, JD:jidewang@gmail.comen_US
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_US
dc.identifier.emailWong, BCY:bcywong@hku.hken_US
dc.identifier.authorityWang, JD=rp00491en_US
dc.identifier.authorityLin, MCM=rp00746en_US
dc.identifier.authorityWong, BCY=rp00429en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.canlet.2005.10.031en_US
dc.identifier.pmid16337741-
dc.identifier.scopuseid_2-s2.0-33746663482en_US
dc.identifier.hkuros115419-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746663482&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume241en_US
dc.identifier.issue2en_US
dc.identifier.spage268en_US
dc.identifier.epage274en_US
dc.identifier.isiWOS:000240908700013-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridXia, HHX=8757161400en_US
dc.identifier.scopusauthoridHe, H=36185495900en_US
dc.identifier.scopusauthoridWang, JD=35309087500en_US
dc.identifier.scopusauthoridGu, Q=24469982400en_US
dc.identifier.scopusauthoridLin, MCM=7404816359en_US
dc.identifier.scopusauthoridZou, B=35228257300en_US
dc.identifier.scopusauthoridYu, LF=8555658200en_US
dc.identifier.scopusauthoridSun, YW=12776261000en_US
dc.identifier.scopusauthoridChan, AOO=7403167965en_US
dc.identifier.scopusauthoridKung, HF=7402514190en_US
dc.identifier.scopusauthoridWong, BCY=7402023340en_US

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