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- Publisher Website: 10.1158/1078-0432.CCR-05-2442
- Scopus: eid_2-s2.0-33744810307
- PMID: 16707623
- WOS: WOS:000237685800037
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Article: Effects of Helicobacter pylori eradication on methylation status of E-cadherin gene in noncancerous stomach
Title | Effects of Helicobacter pylori eradication on methylation status of E-cadherin gene in noncancerous stomach |
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Authors | |
Issue Date | 2006 |
Citation | Clinical Cancer Research, 2006, v. 12 n. 10, p. 3216-3221 How to Cite? |
Abstract | Purpose: Promoter hypermethylation of E-cadherin plays an important role on gastric cancer development. Whereas E-cadherin methylation was frequently detected in the stomach of Helicobacter pylori - infected individuals, we tested whether eradication of H. pylori alters the methylation status of the noncancerous gastric epithelium. Experimental Design: Endoscopic biopsies were taken from the antrum and corpus of H. pylori - infected subjects without gastric cancer. Presence of methylated E-cadherin sequences in the gastric specimens was detected by methylation-specific PCR. Bisulfite DNA sequencing was done to determine the topographical distribution and changes in methylation profiles with H. pylori eradication. Results: Among the 28 H. pylori - infected subjects (median age, 44.5 years), 15 (53.6%) had E-cadherin methylation detected in stomach at baseline. Discordant methylation patterns between the antrum and corpus were noted in six patients. One year after successful H. pylori eradication, there was a significant reduction in the methylation density of the promoter region and exon 1 of the E-cadherin gene as detected by bisulfite DNA sequencing (P < 0.001). Conclusion: Promoter methylation in E-cadherin was frequently detected in the stomach of H. pylori - infected individuals. Eradication of H. pylori might possibly reduce the methylation density in E-cadherin gene and the chance of subsequent neoplastic transformation. © 2006 American Association for Cancer Research. |
Persistent Identifier | http://hdl.handle.net/10722/162984 |
ISSN | 2023 Impact Factor: 10.0 2023 SCImago Journal Rankings: 4.623 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Leung, WK | en_US |
dc.contributor.author | Man, EPS | en_US |
dc.contributor.author | Yu, J | en_US |
dc.contributor.author | Go, MYY | en_US |
dc.contributor.author | To, KF | en_US |
dc.contributor.author | Yamaoka, Y | en_US |
dc.contributor.author | Cheng, VYY | en_US |
dc.contributor.author | Ng, EKW | en_US |
dc.contributor.author | Sung, JJY | en_US |
dc.date.accessioned | 2012-09-05T05:26:14Z | - |
dc.date.available | 2012-09-05T05:26:14Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Clinical Cancer Research, 2006, v. 12 n. 10, p. 3216-3221 | en_US |
dc.identifier.issn | 1078-0432 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/162984 | - |
dc.description.abstract | Purpose: Promoter hypermethylation of E-cadherin plays an important role on gastric cancer development. Whereas E-cadherin methylation was frequently detected in the stomach of Helicobacter pylori - infected individuals, we tested whether eradication of H. pylori alters the methylation status of the noncancerous gastric epithelium. Experimental Design: Endoscopic biopsies were taken from the antrum and corpus of H. pylori - infected subjects without gastric cancer. Presence of methylated E-cadherin sequences in the gastric specimens was detected by methylation-specific PCR. Bisulfite DNA sequencing was done to determine the topographical distribution and changes in methylation profiles with H. pylori eradication. Results: Among the 28 H. pylori - infected subjects (median age, 44.5 years), 15 (53.6%) had E-cadherin methylation detected in stomach at baseline. Discordant methylation patterns between the antrum and corpus were noted in six patients. One year after successful H. pylori eradication, there was a significant reduction in the methylation density of the promoter region and exon 1 of the E-cadherin gene as detected by bisulfite DNA sequencing (P < 0.001). Conclusion: Promoter methylation in E-cadherin was frequently detected in the stomach of H. pylori - infected individuals. Eradication of H. pylori might possibly reduce the methylation density in E-cadherin gene and the chance of subsequent neoplastic transformation. © 2006 American Association for Cancer Research. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Clinical Cancer Research | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Cadherins - Biosynthesis - Genetics - Metabolism | en_US |
dc.subject.mesh | Cell Transformation, Neoplastic | en_US |
dc.subject.mesh | Dna Methylation | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Gastric Mucosa | en_US |
dc.subject.mesh | Helicobacter Infections - Complications - Drug Therapy | en_US |
dc.subject.mesh | Helicobacter Pylori | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Middle Aged | en_US |
dc.subject.mesh | Polymerase Chain Reaction | en_US |
dc.subject.mesh | Promoter Regions, Genetic | en_US |
dc.subject.mesh | Pyloric Antrum - Chemistry | en_US |
dc.subject.mesh | Stomach - Microbiology | en_US |
dc.subject.mesh | Stomach Neoplasms - Etiology - Microbiology - Prevention & Control | en_US |
dc.title | Effects of Helicobacter pylori eradication on methylation status of E-cadherin gene in noncancerous stomach | en_US |
dc.type | Article | en_US |
dc.identifier.email | Leung, WK:waikleung@hku.hk | en_US |
dc.identifier.authority | Leung, WK=rp01479 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1158/1078-0432.CCR-05-2442 | en_US |
dc.identifier.pmid | 16707623 | - |
dc.identifier.scopus | eid_2-s2.0-33744810307 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33744810307&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 12 | en_US |
dc.identifier.issue | 10 | en_US |
dc.identifier.spage | 3216 | en_US |
dc.identifier.epage | 3221 | en_US |
dc.identifier.isi | WOS:000237685800037 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Leung, WK=7201504523 | en_US |
dc.identifier.scopusauthorid | Man, EPS=7004439159 | en_US |
dc.identifier.scopusauthorid | Yu, J=35351306800 | en_US |
dc.identifier.scopusauthorid | Go, MYY=7101882939 | en_US |
dc.identifier.scopusauthorid | To, KF=7101911940 | en_US |
dc.identifier.scopusauthorid | Yamaoka, Y=35200098000 | en_US |
dc.identifier.scopusauthorid | Cheng, VYY=8289922300 | en_US |
dc.identifier.scopusauthorid | Ng, EKW=7201647539 | en_US |
dc.identifier.scopusauthorid | Sung, JJY=24473715000 | en_US |
dc.identifier.issnl | 1078-0432 | - |