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Article: Effects of Helicobacter pylori eradication on methylation status of E-cadherin gene in noncancerous stomach

TitleEffects of Helicobacter pylori eradication on methylation status of E-cadherin gene in noncancerous stomach
Authors
Issue Date2006
Citation
Clinical Cancer Research, 2006, v. 12 n. 10, p. 3216-3221 How to Cite?
AbstractPurpose: Promoter hypermethylation of E-cadherin plays an important role on gastric cancer development. Whereas E-cadherin methylation was frequently detected in the stomach of Helicobacter pylori - infected individuals, we tested whether eradication of H. pylori alters the methylation status of the noncancerous gastric epithelium. Experimental Design: Endoscopic biopsies were taken from the antrum and corpus of H. pylori - infected subjects without gastric cancer. Presence of methylated E-cadherin sequences in the gastric specimens was detected by methylation-specific PCR. Bisulfite DNA sequencing was done to determine the topographical distribution and changes in methylation profiles with H. pylori eradication. Results: Among the 28 H. pylori - infected subjects (median age, 44.5 years), 15 (53.6%) had E-cadherin methylation detected in stomach at baseline. Discordant methylation patterns between the antrum and corpus were noted in six patients. One year after successful H. pylori eradication, there was a significant reduction in the methylation density of the promoter region and exon 1 of the E-cadherin gene as detected by bisulfite DNA sequencing (P < 0.001). Conclusion: Promoter methylation in E-cadherin was frequently detected in the stomach of H. pylori - infected individuals. Eradication of H. pylori might possibly reduce the methylation density in E-cadherin gene and the chance of subsequent neoplastic transformation. © 2006 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/162984
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, WKen_US
dc.contributor.authorMan, EPSen_US
dc.contributor.authorYu, Jen_US
dc.contributor.authorGo, MYYen_US
dc.contributor.authorTo, KFen_US
dc.contributor.authorYamaoka, Yen_US
dc.contributor.authorCheng, VYYen_US
dc.contributor.authorNg, EKWen_US
dc.contributor.authorSung, JJYen_US
dc.date.accessioned2012-09-05T05:26:14Z-
dc.date.available2012-09-05T05:26:14Z-
dc.date.issued2006en_US
dc.identifier.citationClinical Cancer Research, 2006, v. 12 n. 10, p. 3216-3221en_US
dc.identifier.issn1078-0432en_US
dc.identifier.urihttp://hdl.handle.net/10722/162984-
dc.description.abstractPurpose: Promoter hypermethylation of E-cadherin plays an important role on gastric cancer development. Whereas E-cadherin methylation was frequently detected in the stomach of Helicobacter pylori - infected individuals, we tested whether eradication of H. pylori alters the methylation status of the noncancerous gastric epithelium. Experimental Design: Endoscopic biopsies were taken from the antrum and corpus of H. pylori - infected subjects without gastric cancer. Presence of methylated E-cadherin sequences in the gastric specimens was detected by methylation-specific PCR. Bisulfite DNA sequencing was done to determine the topographical distribution and changes in methylation profiles with H. pylori eradication. Results: Among the 28 H. pylori - infected subjects (median age, 44.5 years), 15 (53.6%) had E-cadherin methylation detected in stomach at baseline. Discordant methylation patterns between the antrum and corpus were noted in six patients. One year after successful H. pylori eradication, there was a significant reduction in the methylation density of the promoter region and exon 1 of the E-cadherin gene as detected by bisulfite DNA sequencing (P < 0.001). Conclusion: Promoter methylation in E-cadherin was frequently detected in the stomach of H. pylori - infected individuals. Eradication of H. pylori might possibly reduce the methylation density in E-cadherin gene and the chance of subsequent neoplastic transformation. © 2006 American Association for Cancer Research.en_US
dc.languageengen_US
dc.relation.ispartofClinical Cancer Researchen_US
dc.subject.meshAdulten_US
dc.subject.meshCadherins - Biosynthesis - Genetics - Metabolismen_US
dc.subject.meshCell Transformation, Neoplasticen_US
dc.subject.meshDna Methylationen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastric Mucosaen_US
dc.subject.meshHelicobacter Infections - Complications - Drug Therapyen_US
dc.subject.meshHelicobacter Pylorien_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshPyloric Antrum - Chemistryen_US
dc.subject.meshStomach - Microbiologyen_US
dc.subject.meshStomach Neoplasms - Etiology - Microbiology - Prevention & Controlen_US
dc.titleEffects of Helicobacter pylori eradication on methylation status of E-cadherin gene in noncancerous stomachen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1158/1078-0432.CCR-05-2442en_US
dc.identifier.pmid16707623-
dc.identifier.scopuseid_2-s2.0-33744810307en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33744810307&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume12en_US
dc.identifier.issue10en_US
dc.identifier.spage3216en_US
dc.identifier.epage3221en_US
dc.identifier.isiWOS:000237685800037-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridMan, EPS=7004439159en_US
dc.identifier.scopusauthoridYu, J=35351306800en_US
dc.identifier.scopusauthoridGo, MYY=7101882939en_US
dc.identifier.scopusauthoridTo, KF=7101911940en_US
dc.identifier.scopusauthoridYamaoka, Y=35200098000en_US
dc.identifier.scopusauthoridCheng, VYY=8289922300en_US
dc.identifier.scopusauthoridNg, EKW=7201647539en_US
dc.identifier.scopusauthoridSung, JJY=24473715000en_US

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