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Article: Sapovirus detection by quantitative real-time RT-PCR in clinical stool specimens

TitleSapovirus detection by quantitative real-time RT-PCR in clinical stool specimens
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal Of Virological Methods, 2006, v. 134 n. 1-2, p. 146-153 How to Cite?
AbstractSapovirus (SV) is one of the major causative agents of viral gastroenteritis affecting all age groups worldwide. A new method for the quantitative detection of SV from clinical stool specimens by real-time reverse transcription-polymerase chain reaction (RT-PCR) based on TaqMan ® MGB technology was described. Primers and probe were designed to target the RNA-dependent RNA polymerase/capsid genes junction. Performance of the newly developed assay was validated against a panel of 244 clinical stool specimens collected for patients with gastroenteritis. SV was detected in eight (3.3%) specimens. Phylogenetic analysis of the positive isolates suggested that the assay could detect at least SV genogroups I, II and IV. In addition, the assay had an increased detection rate compared with a widely used conventional RT-PCR assay. Quantitative analysis showed that the assay could detect as low as 10 copies of viral cDNA per reaction. No cross-reactivity with norovirus and rotavirus was observed. In conclusion, the assay is a sensitive and specific method for the detection of SV from clinical stool specimens. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/162969
ISSN
2015 Impact Factor: 1.508
2015 SCImago Journal Rankings: 0.866
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, MCWen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorLam, RKYen_US
dc.contributor.authorChan, PKSen_US
dc.contributor.authorLai, RWMen_US
dc.contributor.authorLeung, WKen_US
dc.date.accessioned2012-09-05T05:26:02Z-
dc.date.available2012-09-05T05:26:02Z-
dc.date.issued2006en_US
dc.identifier.citationJournal Of Virological Methods, 2006, v. 134 n. 1-2, p. 146-153en_US
dc.identifier.issn0166-0934en_US
dc.identifier.urihttp://hdl.handle.net/10722/162969-
dc.description.abstractSapovirus (SV) is one of the major causative agents of viral gastroenteritis affecting all age groups worldwide. A new method for the quantitative detection of SV from clinical stool specimens by real-time reverse transcription-polymerase chain reaction (RT-PCR) based on TaqMan ® MGB technology was described. Primers and probe were designed to target the RNA-dependent RNA polymerase/capsid genes junction. Performance of the newly developed assay was validated against a panel of 244 clinical stool specimens collected for patients with gastroenteritis. SV was detected in eight (3.3%) specimens. Phylogenetic analysis of the positive isolates suggested that the assay could detect at least SV genogroups I, II and IV. In addition, the assay had an increased detection rate compared with a widely used conventional RT-PCR assay. Quantitative analysis showed that the assay could detect as low as 10 copies of viral cDNA per reaction. No cross-reactivity with norovirus and rotavirus was observed. In conclusion, the assay is a sensitive and specific method for the detection of SV from clinical stool specimens. © 2005 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_US
dc.relation.ispartofJournal of Virological Methodsen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCaliciviridae Infections - Virologyen_US
dc.subject.meshCapsiden_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshDna Primersen_US
dc.subject.meshFeces - Virologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastroenteritis - Virologyen_US
dc.subject.meshGenes, Viral - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRna Replicase - Geneticsen_US
dc.subject.meshRna, Viral - Geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Methodsen_US
dc.subject.meshSapovirus - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshViral Plaque Assayen_US
dc.titleSapovirus detection by quantitative real-time RT-PCR in clinical stool specimensen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jviromet.2005.12.013en_US
dc.identifier.pmid16427707-
dc.identifier.scopuseid_2-s2.0-33646137373en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646137373&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume134en_US
dc.identifier.issue1-2en_US
dc.identifier.spage146en_US
dc.identifier.epage153en_US
dc.identifier.isiWOS:000238005500020-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChan, MCW=36941299700en_US
dc.identifier.scopusauthoridSung, JJY=24473715000en_US
dc.identifier.scopusauthoridLam, RKY=7101916902en_US
dc.identifier.scopusauthoridChan, PKS=7403497792en_US
dc.identifier.scopusauthoridLai, RWM=8916364700en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.citeulike3874011-

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