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Article: The Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II

TitleThe Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II
Authors
Issue Date2006
Citation
Molecular And Cellular Biology, 2006, v. 26 n. 8, p. 3135-3148 How to Cite?
AbstractThe Paf1 complex (Paf1C) interacts with RNA polymerase II (Pol II) and promotes histone methylation of transcribed coding sequences, but the mechanism of Paf1C recruitment is unknown. We show that Paf1C is not recruited directly by the activator Gcn4p but is dependent on preinitiation complex assembly and Ser5 carboxy-terminal domain phosphorylation for optimal association with ARG1 coding sequences. Importantly, Spt4p is required for Paf1C occupancy at ARG1 (and other genes) and for Paf1C association with Ser5-phosphorylated Pol II in cell extracts, whereas Spt4p-Pol II association is independent of Paf1C. Since spt4Δ does not reduce levels of Pol II at ARG1, Ser5 phosphorylation, or Paf1C expression, it appears that Spt4p (or its partner in DSIF, Spt5p) provides a platform on Pol II for recruiting Paf1C following Ser5 phosphorylation and promoter clearance. spt4Δ reduces trimethylation of Lys4 on histone H3, demonstrating a new role for yeast DSIF in promoting a Paf1C-dependent function in elongation.
Persistent Identifierhttp://hdl.handle.net/10722/162962
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQiu, Hen_US
dc.contributor.authorHu, Cen_US
dc.contributor.authorWong, CMen_US
dc.contributor.authorHinnebusch, AGen_US
dc.date.accessioned2012-09-05T05:25:56Z-
dc.date.available2012-09-05T05:25:56Z-
dc.date.issued2006en_US
dc.identifier.citationMolecular And Cellular Biology, 2006, v. 26 n. 8, p. 3135-3148en_US
dc.identifier.issn0270-7306en_US
dc.identifier.urihttp://hdl.handle.net/10722/162962-
dc.description.abstractThe Paf1 complex (Paf1C) interacts with RNA polymerase II (Pol II) and promotes histone methylation of transcribed coding sequences, but the mechanism of Paf1C recruitment is unknown. We show that Paf1C is not recruited directly by the activator Gcn4p but is dependent on preinitiation complex assembly and Ser5 carboxy-terminal domain phosphorylation for optimal association with ARG1 coding sequences. Importantly, Spt4p is required for Paf1C occupancy at ARG1 (and other genes) and for Paf1C association with Ser5-phosphorylated Pol II in cell extracts, whereas Spt4p-Pol II association is independent of Paf1C. Since spt4Δ does not reduce levels of Pol II at ARG1, Ser5 phosphorylation, or Paf1C expression, it appears that Spt4p (or its partner in DSIF, Spt5p) provides a platform on Pol II for recruiting Paf1C following Ser5 phosphorylation and promoter clearance. spt4Δ reduces trimethylation of Lys4 on histone H3, demonstrating a new role for yeast DSIF in promoting a Paf1C-dependent function in elongation.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Biologyen_US
dc.subject.meshChromatin Immunoprecipitationen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshNuclear Proteins - Chemistry - Genetics - Metabolism - Physiologyen_US
dc.subject.meshProtein Subunits - Metabolismen_US
dc.subject.meshRna Polymerase Ii - Metabolismen_US
dc.subject.meshSaccharomyces Cerevisiae - Genetics - Metabolismen_US
dc.subject.meshSaccharomyces Cerevisiae Proteins - Chemistry - Genetics - Metabolism - Physiologyen_US
dc.subject.meshTranscriptional Elongation Factors - Chemistry - Physiologyen_US
dc.titleThe Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase IIen_US
dc.typeArticleen_US
dc.identifier.emailWong, CM:wispwong@hkucc.hku.hken_US
dc.identifier.authorityWong, CM=rp01489en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/MCB.26.8.3135-3148.2006en_US
dc.identifier.pmid16581788en_US
dc.identifier.scopuseid_2-s2.0-33645814013en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645814013&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume26en_US
dc.identifier.issue8en_US
dc.identifier.spage3135en_US
dc.identifier.epage3148en_US
dc.identifier.isiWOS:000236657600025-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridQiu, H=7201608807en_US
dc.identifier.scopusauthoridHu, C=7404570409en_US
dc.identifier.scopusauthoridWong, CM=18134632400en_US
dc.identifier.scopusauthoridHinnebusch, AG=7005728566en_US
dc.identifier.citeulike4324605-

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