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Article: The existence of a putative regulatory element in 3′-untranslated region of proto-oncogene HOX11's mRNA

TitleThe existence of a putative regulatory element in 3′-untranslated region of proto-oncogene HOX11's mRNA
Authors
Issue Date2005
Citation
Journal Of Biochemistry And Molecular Biology, 2005, v. 38 n. 4, p. 500-506 How to Cite?
AbstractHOX11 encodes a homeodomain-containing transcription factor which directs the development of the spleen during embryogenesis. While HOX11 expression is normally silenced through an unknown mechanism in all tissues by adulthood, the deregulation of HOX11 expression is associated with leukemia, such as T-cell acute lymphoblastic leukemia. The elucidation of regulatory elements contributing to the molecular mechanism underlying the regulation of HOX11 gene expression is of great importance. Previous reports of HOX11 regulatory elements mainly focused on the 5′-flanking region of HOX11 on the chromosome related to transcriptional control. To expand the search of putative ds-elements involved in HOX11 regulation at the post-transcriptional level, we analyzed HOX11 mRNA 3′-untranslated region (3′UTR) and found an AU-rich region. To characterize this AU-rich region, in vitro analysis of HOX11 mRNA 3′UTR was performed with human RNA-binding protein HuR, which interacts with AU-rich element (ARE) existing in the 3′UTR of many growth factors' and cytokines' mRNAs. Our results showed that the HOX11 mRNA 3′UTR can specifically bind with human HuR protein in vitro. This specific binding could be competed effectively by typical ARE containing RNA. After the deletion of the AU-rich region present in the HOX11 mRNA 3′UTR, the interaction of HOX11 mRNA 3′UTR with HuR protein was abolished. These findings suggest that HOX11 mRNA 3′UTR contains cis-acting element which shares similarity in the action pattern with ARE-HuR interactions and may involve in the post-transcriptional regulation of the HOX11 gene.
Persistent Identifierhttp://hdl.handle.net/10722/162921
ISSN
2009 Impact Factor: 2.021
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Yen_US
dc.contributor.authorJiang, ZZen_US
dc.contributor.authorChen, HXen_US
dc.contributor.authorLeung, WKen_US
dc.contributor.authorSung, JJYen_US
dc.contributor.authorMa, WJen_US
dc.date.accessioned2012-09-05T05:25:21Z-
dc.date.available2012-09-05T05:25:21Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Biochemistry And Molecular Biology, 2005, v. 38 n. 4, p. 500-506en_US
dc.identifier.issn1225-8687en_US
dc.identifier.urihttp://hdl.handle.net/10722/162921-
dc.description.abstractHOX11 encodes a homeodomain-containing transcription factor which directs the development of the spleen during embryogenesis. While HOX11 expression is normally silenced through an unknown mechanism in all tissues by adulthood, the deregulation of HOX11 expression is associated with leukemia, such as T-cell acute lymphoblastic leukemia. The elucidation of regulatory elements contributing to the molecular mechanism underlying the regulation of HOX11 gene expression is of great importance. Previous reports of HOX11 regulatory elements mainly focused on the 5′-flanking region of HOX11 on the chromosome related to transcriptional control. To expand the search of putative ds-elements involved in HOX11 regulation at the post-transcriptional level, we analyzed HOX11 mRNA 3′-untranslated region (3′UTR) and found an AU-rich region. To characterize this AU-rich region, in vitro analysis of HOX11 mRNA 3′UTR was performed with human RNA-binding protein HuR, which interacts with AU-rich element (ARE) existing in the 3′UTR of many growth factors' and cytokines' mRNAs. Our results showed that the HOX11 mRNA 3′UTR can specifically bind with human HuR protein in vitro. This specific binding could be competed effectively by typical ARE containing RNA. After the deletion of the AU-rich region present in the HOX11 mRNA 3′UTR, the interaction of HOX11 mRNA 3′UTR with HuR protein was abolished. These findings suggest that HOX11 mRNA 3′UTR contains cis-acting element which shares similarity in the action pattern with ARE-HuR interactions and may involve in the post-transcriptional regulation of the HOX11 gene.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Biochemistry and Molecular Biologyen_US
dc.subject.mesh3' Untranslated Regions - Geneticsen_US
dc.subject.meshAntigens, Surface - Genetics - Metabolismen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshElectrophoretic Mobility Shift Assayen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGlutathione Transferase - Genetics - Metabolismen_US
dc.subject.meshHomeodomain Proteins - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshProto-Oncogene Proteins - Geneticsen_US
dc.subject.meshRna, Messenger - Chemistryen_US
dc.subject.meshRna-Binding Proteins - Genetics - Metabolismen_US
dc.subject.meshRecombinant Fusion Proteins - Genetics - Metabolismen_US
dc.subject.meshRepetitive Sequences, Nucleic Aciden_US
dc.subject.meshTranscription, Geneticen_US
dc.titleThe existence of a putative regulatory element in 3′-untranslated region of proto-oncogene HOX11's mRNAen_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid16053719-
dc.identifier.scopuseid_2-s2.0-29844440280en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-29844440280&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume38en_US
dc.identifier.issue4en_US
dc.identifier.spage500en_US
dc.identifier.epage506en_US
dc.identifier.isiWOS:000230910100018-
dc.identifier.scopusauthoridLi, Y=26643413600en_US
dc.identifier.scopusauthoridJiang, ZZ=55202695300en_US
dc.identifier.scopusauthoridChen, HX=7501613108en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.scopusauthoridSung, JJY=24473715000en_US
dc.identifier.scopusauthoridMa, WJ=36071632300en_US

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